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Identification of cells in S phase using the Cell Proliferation Fluorescence Kit and IN Cell Analyzer 1000

determine the proportion of cells in S phase (Figs 3 and 4).

The signal intensity of Cy5 localized BrdU is well preserved in a fixed-cell format and quality data can be obtained from fixed-cell plates stored at +4 °C for up to one week (data not shown).

The assay, in conjunction with automated sub-cellular imaging platforms, is ideally suited for high-throughput primary or secondary screening to uncover novel anticancer drugs and use by toxicologists to establish whether lead compounds adversely effect the rate of cell division.



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