Navigation Links
Identification of cells in S phase using the Cell Proliferation Fluorescence Kit and IN Cell Analyzer 1000

000

Cell Proliferation Fluorescence Kit 25-9001-89


Other materials required
U2OS (human osteosarcoma) epithelial cells (ATCC, HTB 96) McCoys growth medium:
5A medium modified (Sigma, M-8403) containing:
10% (v/v) FBS (Sigma, F-9423),
1% (v/v) L-Glutamine (Invitrogen, 25030-024) and
1% (v/v) Penicillin/Streptomycin (Invitrogen, 15140-122)

Hoechst nuclear dye (Molecular Probes, 33342)

Triton X-100 (Sigma)

4% Formalin solution (Sigma, HT50-1-2)

PBS (Gibco, 14190-094)

96-well assay plate (Greiner, 655090)

PBS tablets (Sigma, P-4417) used to prepare 2× PBS solution

Fixing solution containing:
4% Formalin solution, 5 ml
Sterile water, 5 ml
2× PBS, 10 ml
Triton X-100, 20 ml
N.B. ensure Triton is properly dissolved before use.


Method
Day 1.

1. Prior to seeding ensure the U2OS cells are sub-confluent.

2. Seed U2OS cells into a 96-well assay plate at 7000 cells/well (100 ml aliquots) and incubate at 37 °C overnight in 5% CO2 together with various dilutions of test substance (e.g., mitogens, cytostatic drugs, growth factors etc.).

N.B. In some cases it may be preferable to add the test substance on Day 2 and incubate for a shorter length of time.

Day 2.
3. Dilute BrdU labeling reagent 1:250 with Growth medium and add 100 ml aliquots to appropriate wells to give a final dilution of 1:500 in the well.

4. Incubate plate for 1 h at 37 °C in 5% CO2.

5. Discard medium and wash the cells twice with ste
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Identification of Differentially Expressed Gene Products with the CastAway System*
2. An Optimal DNA Microarray Substrate for the Identification of Fetal and Somatic Liver Stem Cells of the Rat
3. Identification of Low-Level Oxidation Products in Calmodulin Using NanoSpray Ion Trap Mass Spectrometry
4. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
5. Protect RNA and Improve Target Cell Identification
6. Identification of Disease Causing Mutations in Phenylketonuria by Denaturing Gradient Gel Electrophoresis Using the DCode System
7. Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A
8. Identification of Insecticides and Herbicides by LC/MS/MS Using the API 2000 LC/MS/MS System
9. Identification of Phase I and Phase II Metabolites of Buspirone on the Q TRAP LC/MS/MS System
10. ESR1 Gene Expression in Oncology and Metabolic Diseases Using the ASCENTATM System Target Validation and Identification of Novel Disease Indications
11. Easy preparation of total RNA from cultured cells with TRIzol and Eppendorf Phase Lock Gel
Post Your Comments:
(Date:8/3/2015)... 2015  MiMedx Group, Inc. (NASDAQ: MDXG ), ... and patent-protected processes to develop and market advanced products ... Sports Medicine, Ophthalmic and Dental sectors of healthcare, announced ... Annual Canaccord|Genuity Growth Conference in Boston, ... CEO, William C. Taylor , President and COO, ...
(Date:8/3/2015)... -- Merck KGaA, Darmstadt, Germany ,  a ... the healthcare, life science and performance materials sectors, announced ... and launch of promising new fullerene derivatives for the ... Germany , is pursuing many projects ... Novel derivatives: highly stable, easy to process and cost ...
(Date:8/3/2015)... 2015  Aethlon Medical, Inc. (Nasdaq: AEMD ), ... life-threatening diseases, today announced that it will host its ... on Thursday, August 13, 2015 at 4:30 p.m. Eastern ... the release of fiscal 2016 first quarter results.  A ... two hours after the call at the Investor Relations ...
(Date:8/3/2015)... Aug. 3, 2015   Intrexon Corporation (NYSE: ... half and second quarter 2015 financial results after the ... The Company will host a conference call at 5:30 ... general business update. The conference call may ... (International), and asking to join the "Intrexon Corporation Call."  ...
Breaking Biology Technology:MiMedx To Present At 35th Annual Canaccord|Genuity Growth Conference 2Merck KGaA, Darmstadt, Germany, and Nano-C Present Enhanced Materials for Organic Photovoltaics 2Merck KGaA, Darmstadt, Germany, and Nano-C Present Enhanced Materials for Organic Photovoltaics 3Merck KGaA, Darmstadt, Germany, and Nano-C Present Enhanced Materials for Organic Photovoltaics 4Aethlon Medical To Host First Quarter Fiscal 2016 Investor Call on August 13, 2015 2Intrexon to Announce First Half and Second Quarter 2015 Financial Results and Host Conference Call on August 10th 2
... Hollingsworth & Vose,Company (H&V), a leading ... introduced its HVision(TM) technology platform, a collaborative,process ... of,breakthrough products. "HVision will provide H&V ... a new generation of products that exceed ...
... Corporation (OTC,Bulletin Board: KDUS.OB) announced today financial results for the ... the third quarter of 2007 and for the same period,in ... $240,227, compared to,net income of $200,335 for the same period ... third quarter of 2007 and for the same period in,2006. ...
... Stock Exchange: EVT) Reported Today Results for,the First ... OXFORD, England, November 13 /PRNewswire-FirstCall/,-- Highlights: ... - Compelling results achieved in two proof-of-concept ... 201 - Sale of the Chemical Development ...
Cached Biology Technology:H&V Introduces HVision Technology Platform to Accelerate Development of Breakthrough Products 2Cadus Reports Third Quarter 2007 Results 2Cadus Reports Third Quarter 2007 Results 3Cadus Reports Third Quarter 2007 Results 4Evotec Reports Third Quarter 2007 Results 2Evotec Reports Third Quarter 2007 Results 3Evotec Reports Third Quarter 2007 Results 4Evotec Reports Third Quarter 2007 Results 5Evotec Reports Third Quarter 2007 Results 6Evotec Reports Third Quarter 2007 Results 7Evotec Reports Third Quarter 2007 Results 8
(Date:7/9/2015)... 9, 2015  Synaptics Inc. (NASDAQ: ... interface solutions, today announced a new Match-in-Sensor™ ... hardware encapsulated fingerprint sensor and matching solution. ... literally off the grid, isolating fingerprint image ... the fingerprint sensor to provide ultimate protection ...
(Date:7/7/2015)... 30, 2015 Research ... addition of the "Capacitive Fingerprint Sensors Patent ... To this date, fingerprint sensing technology is the ... sensors are well developed. This patent landscape focuses ... The domain of capacitive fingerprint sensors is ...
(Date:7/2/2015)... Fingerprint Cards has received an order for its ... distributor World Peace Industrial Group (WPI), part of WPG Holdings. ... . Deliveries are planned to take place during Q3 ... manufacturers in Asia . The order value ... of approximately 2,200 MSEK for 2015. Jörgen Lantto, ...
Breaking Biology News(10 mins):Synaptics Announces World's First Match-in-Sensor Fingerprint Authentication Technology 2Synaptics Announces World's First Match-in-Sensor Fingerprint Authentication Technology 3Worldwide Capacitive Fingerprint Sensors Patent Landscape Market Report 2015-2020 - Main IP Holders include Seiko Epson, AuthenTec, Sony and STMicroelectronics 2FPC Receives Order for Touch Fingerprint Sensors of 268 MSEK 2
... collective efforts to develop new drugs, the pharmaceuticals industry ... application of natural biological transformation processes. One of the ... cells in a bioreactor filled with a liquid culture ... regulations, the conditions inside the bioreactor need to be ...
... various Earth system science disciplines recently gathered to address ... in the future? Of course, possible answers to ... changing climatic conditions were to alter local vegetation, how ... Answering these questions with certainty would allow us to ...
... of BioScience, researchers present a sophisticated new ... ethanol on greenhouse gas emissions. The study, conducted by ... focuses on how mandated increases in production of the ... domestically and elsewhere. In response to the increased demand ...
Cached Biology News:Clean sampling from bioreactors 2Networking initiative to support interdisciplinary research 2Networking initiative to support interdisciplinary research 3More maize ethanol may boost greenhouse gas emissions 2
Rabbit polyclonal to Glutathione S Transferase alpha ( Abpromise for all tested applications). Antigen: Full length protein: Human Glutathione S Transferase alpha. Entrez GeneID: 2938 Swi...
Affinity Purified Rabbit Anti-mouse sRANKL Biotinylated Polyclonal Antibody...
Mouse anti-IkBa/MAD-3 Class: Antibody Product Group: Signalling molecules and phospho-specific Antibody...
Mouse monoclonal [002-49] to C1s ( Abpromise for all tested applications). entrezGeneID: 716 SwissProtID: P09871...
Biology Products: