Navigation Links
Identification of cells in S phase using the Cell Proliferation Fluorescence Kit and IN Cell Analyzer 1000


The cell cycle is of key importance to many areas of drug discovery. On the one hand this fundamental process provides the opportunity to discover new therapeutic targets for anti-cancer agents, and on the other hand drugs and targets in other therapeutic areas must be tested for undesirable effects on the cell cycle.

The Cell Proliferation Fluorescence Kit is a precise, fast, and simple fluorescence assay to quantitate cell proliferation, study the regulation of DNA synthesis, or observe the degree of inhibition of cell progression through S phase. It is based on the measurement of 5-bromo-2'-deoxyuridine (BrdU) incorporation during DNA synthesis in proliferating cells.

BrdU (an analog of the DNA precursor thymidine) is incorporated into newly synthesized DNA by cells entering and progressing through the S (DNA synthesis) phase of the cell cycle. The incorporated BrdU is detected with a specific anti-BrdU monoclonal antibody, followed by a Cy™5 labeled antibody to mouse immunoglobulin, giving a fluorescent signal at sites of BrdU incorporation. This provides an excellent marker for identifying cells that are in S phase, and also for determining the proportion of cells in S phase of the cell cycle.

The intensity of nuclear-associated Cy5 fluorescence is measured using an IN Cell Analyzer 1000 and the data analyzed using an Object Intensity Analysis Module.

The use of a Cy5 red-shifted fluor enables this technology to be multiplexed with green fluorescent protein (GFP). This assay provides a high-throughput method for the identification and analysis of cells that have actively synthesized DNA.


Materials
Products used

IN Cell Analyzer 1000 25-8010-26

Object Intensity Analysis Module 25-8010-56
for IN Cell Analyzer 1
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Identification of Differentially Expressed Gene Products with the CastAway System*
2. An Optimal DNA Microarray Substrate for the Identification of Fetal and Somatic Liver Stem Cells of the Rat
3. Identification of Low-Level Oxidation Products in Calmodulin Using NanoSpray Ion Trap Mass Spectrometry
4. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
5. Protect RNA and Improve Target Cell Identification
6. Identification of Disease Causing Mutations in Phenylketonuria by Denaturing Gradient Gel Electrophoresis Using the DCode System
7. Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A
8. Identification of Insecticides and Herbicides by LC/MS/MS Using the API 2000 LC/MS/MS System
9. Identification of Phase I and Phase II Metabolites of Buspirone on the Q TRAP LC/MS/MS System
10. ESR1 Gene Expression in Oncology and Metabolic Diseases Using the ASCENTATM System Target Validation and Identification of Novel Disease Indications
11. Easy preparation of total RNA from cultured cells with TRIzol and Eppendorf Phase Lock Gel
Post Your Comments:
(Date:8/30/2015)... England , August 31, 2015 ...   einer Frau   für chirurgische   Behandlung     ... von 7-90   werden für eine   verheerende ... 1200 Frauen behandeln und die Anzahl ausgebildeter Chirurgen in ... Astellas gab heute bekannt, dass  Action on Fistula ...
(Date:8/28/2015)... (PRWEB) , ... August 28, 2015 , ... ... Personalized Medicine, presented two studies at the International Spine Intervention Society’ ... 28th, 2015 through to August 1st, 2015. With a focus on evidence-based research ...
(Date:8/28/2015)... ... August 28, 2015 , ... ... surgeon, Wade McKenna, DO, announced today that the use of sterile, dehydrated amniotic ... complete healing of an otherwise non-healing surgical knee wound. , The case involved ...
(Date:8/27/2015)... (PRWEB) , ... August 27, 2015 , ... Capital ... solve complex business problems, is pleased to announce their ranking as one of the ... ranking of the fastest-growing private companies across the nation. , “It is truly an ...
Breaking Biology Technology:Über 550 Frauen, deren Leben durch "Action on Fistula" transformiert wurde 2Über 550 Frauen, deren Leben durch "Action on Fistula" transformiert wurde 3Über 550 Frauen, deren Leben durch "Action on Fistula" transformiert wurde 4Über 550 Frauen, deren Leben durch "Action on Fistula" transformiert wurde 5Proove Biosciences Presents New Research Study at the International Spine Intervention Society’s Annual Scientific Meeting 2Proove Biosciences Presents New Research Study at the International Spine Intervention Society’s Annual Scientific Meeting 3Proove Biosciences Presents New Research Study at the International Spine Intervention Society’s Annual Scientific Meeting 4Dallas-Fort Worth Stem Cell Researchers Use Amniotic Tissue To Successfully Treat Non-Healing Surgical Wound 2Dallas-Fort Worth Stem Cell Researchers Use Amniotic Tissue To Successfully Treat Non-Healing Surgical Wound 3Dallas-Fort Worth Stem Cell Researchers Use Amniotic Tissue To Successfully Treat Non-Healing Surgical Wound 4Capital Edge Consulting Makes Inc. 5000 List of America’s Fastest-Growing Private Companies 2Capital Edge Consulting Makes Inc. 5000 List of America’s Fastest-Growing Private Companies 3
... Corp . President Paul Shain spoke on his ... Madisons monthly meeting. According to Shain, Berbees unique principles and ... dot-com era as other information technology-focused companies failed. , ,Started ... founded on the dogma of creating an environment for success, ...
... Internet access is now a reality for one rural ... system. Toronto-based WaveRider Communications Inc. is working ... Oakdale Electric Cooperative to implement its Last Mile ... to broadband Internet services. , ,The response in Mauston ...
... a talk at my local elementary school given by Peter ... of systems thinking. Systems thinking is a conceptual framework, body ... years, to train people to see the big picture ... how to make systematic change successfully. The essence of systems ...
Cached Biology Technology:Berbees success is based on companys history and culture 2Berbees success is based on companys history and culture 3Berbees success is based on companys history and culture 4Rural Wisconsin town gets high-speed Internet access 2Outsourcing: Net gain or shifting the pain? 2Outsourcing: Net gain or shifting the pain? 3
(Date:8/10/2015)... TELTOW, Germany , August 10, 2015 ... a world leader in Eye Tracking Technology for more ... OEM Eye Tracking Platform for integration into all consumer ... reference designs for seamless integration of eye tracking into ... HMDs and augmented reality smart glasses. Omnivision,s leading sensor ...
(Date:8/6/2015)... , Aug. 6, 2015  Today, U.S. ... visit Crossmatch ™ to learn firsthand how ... protect data. Crossmatch is a world leader in ... and expertise are helping federal agencies and commercial ... security.   "The vast majority of ...
(Date:8/6/2015)... SAN JOSE, Calif. , Aug. 6, 2015 ... the leading developer of human interface solutions, today ... and TouchPad technologies on Windows 10. Microsoft leveraged ... fundamental to the operating system. ... and drivers are fully certified with Microsoft,s Precision ...
Breaking Biology News(10 mins):SMI Completes OEM Eye Tracking Platform to Cover All Consumer Displays 2SMI Completes OEM Eye Tracking Platform to Cover All Consumer Displays 3Rep. Honda to Visit Crossmatch in Redwood City 2Synaptics Chosen for Human Interface Co-Development on Windows 10 2
... 27, 2013  Definiens, the global leader of image and ... it has appointed an Advisory Board comprising renowned industry ... its market expansion strategy, Definiens established the new Board ... tissue diagnostics and clinical digital pathology. Members of the ...
... caffeine, but scientists now have genetically engineered E. ... report in the journal ACS Synthetic Biology ... that promises practical uses ranging from decontamination of wastewater ... Barrick and colleagues note that caffeine and related chemical ...
... and the University of Oxford have been able to give ... provide paternal care. When the conditions are right, this strategy ... put a lot of effort into caring for offspring that ... sense at all because natural selection should design males to ...
Cached Biology News:Gilde Healthcare III Portfolio Company Definiens Establishes Advisory Board 2Gilde Healthcare III Portfolio Company Definiens Establishes Advisory Board 3Why sticking around is sometimes the better choice 2
Goat polyclonal to Centaurin alpha 2**...
... immobilization can be coupled with a ligand such ... create an affinity matrix of choice. Affi-Gel 10 ... amines. Affi-Gel 10 gel couples neutral or basic ... 15 gel couples acidic proteins with pI less ...
Rabbit anti Mouse ZIC2 Preservative: NaN3...
Monocarboxylate (lactate) transporter 2...
Biology Products: