Broad linear dynamic range provides true triple quadrupole quantitation performance and enhances identification of ions in complex matrices
Information-rich MS/MS and MS3 spectra with important low mass structural ions provide confidence in results
Metabolite ID application software makes it easy to go straight to the answers
Rat liver microsomal incubate samples were injected on a Hypersil- Betasil C18 column (1 x 100 mm). Samples were eluted using Shimadzu 10 AD pumps with a gradient of water/methanol, from 10 to 90% methanol over 10 minutes at a flow rate of 70 μL/min. Direct injection (5 μL) on column was performed using a PE Series 200 autosampler. A TurboIonSpray source was operated at 350 C on the Q TRAP system.
Results and Discussion
The new Q TRAP LC/MS/MS system combines superior ion trap capabilities with triple quadrupole performance to provide a new level of performance and versatility in LC/MS for metabolite identification. The patented hybrid linear design enhances sensitivity, resolution, and duty cycle for MS and MS/MS full scan modes, and combines these features with the linear dynamic range and scan functions of a triple quadrupole instrument.
Add Metabolite ID application software and get automated data acquisition and processing, with a range of options to ensure maximum productivity. Metabolite ID application software leverages the Q TRAP system technology and IDA to provide rapid, high-confidence results in the fewest number of experiments.
Identifies predefined and userdefined Phase I and Phase II transformations
Detects unexpected metabolites by recognition of isotope ratio patterns