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Identification of Low-Level Oxidation Products in Calmodulin Using NanoSpray ,,, Ion Trap Mass Spectrometry

ata Dependent Dynamic Exclusion and MSn has allowed definitive characterization of peptide sequence and oxidation products in a single, rapid experiment, illustrating the power of the ion trap for the identification of post-translational modifications.

References

Gao, J.; Yin, D.H.; Yao, Y.; Williams, T.D.; Squier, T.C. Progressive decline in the ability of calmodulin isolated from aged brain to activate the plasma membrane Ca2-ATPase. Biochemistry 37: 9536-9548; 1998.
Hhmer, A.F.R.; (1997) Peroxynitrite Mediated Methionine Oxidation of Calmodulin: Chemistry and Biological Consequences, Ph.D. Thesis, The University of Kansas.
Walsh, M.; Stevens, F.C. Chemical Modification Studies on the Ca2-Dependent Protein Modulator: The Role of Methionine Residues in the Activation of Cyclic Nucleotide Phosphodiesterase. Biochemistry 17: 3924-3928; 1978.






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