Navigation Links
Identification of Low-Level Oxidation Products in Calmodulin Using NanoSpray ,,, Ion Trap Mass Spectrometry

Andreas Hhmer, Helen Tran, and William S. Hancock

Protein Oxidation

The data presented here can be acquired using any Thermo Finnigan LCQTMion trap mass spectrometer with MSn capability.

Introduction

Calmodulin (CaM) is the primary high-affinity Ca2+-binding protein present in most eukaryotic cells. It regulates a variety of cellular Ca2+-dependent signaling pathways and plays a role in many cellular events, including protein phosphorylation. Ca2+binding induces a large conformational change in CaM that exposes methionine (Met) residues for interaction with CaM-binding enzymes that are otherwise inaccessible. CaM (MW 16706.40) has an unusual amino acid composition consisting of 148 amino acids with a high proportion of acidic residues (>34%) resulting in an isoelectric point around 3.9. CaM is particularly rich in Met residues, which account for more than 5% of its total composition, but it lacks significant amounts of other oxidatively sensitive amino acids such as cysteine, histidine and tryptophan. Evidence presented recently suggests that Met residues in CaM undergo an age-dependent modification to methionine sulfoxide (MetSO) in vivo1. The biological consequences of this oxidation can vary depending on the specific site of the oxidation: MetSO at the Nterminal end of CaM had an effect on CaM-dependent nitric oxide synthase (type-I) whereas MetSO near the C-terminus affected CaM-dependent cyclic phosphodiesterase. 2,3 For a range of biochemical studies, the detailed characterization of the age-related oxidative changes in CaM is important. Typically, this analysis
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Identification of Differentially Expressed Gene Products with the CastAway System*
2. An Optimal DNA Microarray Substrate for the Identification of Fetal and Somatic Liver Stem Cells of the Rat
3. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
4. Protect RNA and Improve Target Cell Identification
5. Identification of Disease Causing Mutations in Phenylketonuria by Denaturing Gradient Gel Electrophoresis Using the DCode System
6. Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A
7. Identification of cells in S phase using the Cell Proliferation Fluorescence Kit and IN Cell Analyzer 1000
8. Identification of Insecticides and Herbicides by LC/MS/MS Using the API 2000 LC/MS/MS System
9. Identification of Phase I and Phase II Metabolites of Buspirone on the Q TRAP LC/MS/MS System
10. ESR1 Gene Expression in Oncology and Metabolic Diseases Using the ASCENTATM System Target Validation and Identification of Novel Disease Indications
11. High-Throughput System Generates Ultra-Pure PCR Products Suitable for All Applications
Post Your Comments:
(Date:7/1/2015)... , July 1, 2015   Decision ... frequently cited advantages of Otezla are its oral ... profile, as reported by surveyed rheumatologists. Otezla was ... for the treatment of active psoriatic arthritis in ... current standard of care includes conventional oral treatments ...
(Date:7/1/2015)... Colo. and BANGALORE, India ... global genomic profiling company that uses next generation sequencing ... of Scott A. Storrer as chief executive ... CEO, he will be responsible for all strategy, business ... Chandru , who has served as Strand,s chairman and ...
(Date:7/1/2015)... Bermuda , July 1, 2015  Axovant ... company focused on the treatment of dementia, today ... briefing on July 22 at 5:45 p.m. EDT ... the Alzheimer,s Association International Conference 2015 (AAIC). ... remarks: , Dr. Lawrence Friedhoff ...
(Date:7/1/2015)... (PRWEB) , ... July 01, 2015 , ... A newly ... new ideas to lower the barriers to innovation in life sciences R&D. The Pistoia ... to two cash prizes of US$15,000 and a period of expert mentorship from a ...
Breaking Biology Technology:Surveyed Rheumatologists Report That Otezla's Oral Administration is its Single Greatest Advantage Over Currently Available Treatment Options 2Surveyed Rheumatologists Report That Otezla's Oral Administration is its Single Greatest Advantage Over Currently Available Treatment Options 3Strand Life Sciences Names Scott A. Storrer CEO as Company Continues Global Expansion with Focus on Precision Medicine Product 2Strand Life Sciences Names Scott A. Storrer CEO as Company Continues Global Expansion with Focus on Precision Medicine Product 3Axovant Sciences To Host Investor Briefing And Live Webcast During AAIC 2015 Meeting 2Axovant Sciences To Host Investor Briefing And Live Webcast During AAIC 2015 Meeting 3Pistoia Alliance Launches Startup Challenge 2Pistoia Alliance Launches Startup Challenge 3
... Dec. 19 BioForm Medical,Inc. (Nasdaq: BFRM ) ... a company overview at the upcoming 26th Annual,JPMorgan Healthcare ... will take place on Monday, January 7th, 2008 at ... Francis Hotel in San Francisco,CA., A live and ...
... Instrumentation Laboratory,(IL) today announced that its three-year ... Purchasing Group, LP (HealthTrust), a,leading group purchasing ... members. Consorta, another leading healthcare group purchasing,organization, ... as the sixth equity owner of HealthTrust, ...
... III von Willebrand Disease, a serious, hereditary ... bleeding disorder, found in region, ... today,announced that Iran,s Ministry of Health and Medical Education (MOHME) ... an exclusive,basis throughout Iran, beginning immediately. Haemate P is indicated ...
Cached Biology Technology:BioForm Medical to Present at the 2008 JPMorgan Healthcare Conference on January 7, 2008 2HealthTrust Expands Contract With Instrumentation Laboratory for Critical Care Portfolio to Include Consorta 2CSL Behring Expands Into Middle East Market with Haemate(R) P 2CSL Behring Expands Into Middle East Market with Haemate(R) P 3
(Date:6/24/2015)... 2015 Research and ... the "Huawei Ascend Mate 7 Fingerprint Sensor ... offering. Huawei,s technological choice for the ... than Apple,s and Samsung,s one. The Ascend Mate ... Fingerprint Cards, a main actor in capacitive sensing ...
(Date:6/23/2015)... June 23, 2015  Crossmatch™, a leading ... today announced enhanced functionality of its DigitalPersona ... The enhancements build on the native flexibility ... Altus platform and provide expanded mobile credential ... In today,s environment of increasing cyber-attacks and ...
(Date:6/17/2015)... 2015  Synaptics Inc. (NASDAQ: SYNA ), ... that Xiaomi, one of the world,s largest mobile ... ® family of capacitive touchscreen solutions and ... for its latest smartphones, the Xiaomi Mi Note ... in-cell display solutions and DDICs in discrete display ...
Breaking Biology News(10 mins):Huawei Ascend Mate 7 Fingerprint Sensor - Reverse Costing Analysis 2Crossmatch Releases Enhanced DigitalPersona Altus Advanced Authentication Functionality 2Synaptics Touchscreen and Display Driver IC Solutions Power Xiaomi's Latest Flagship Smartphones 2Synaptics Touchscreen and Display Driver IC Solutions Power Xiaomi's Latest Flagship Smartphones 3
... partnership with Island Press, the leading nonprofit environmental ... for the next two years. In this agreement, ... award-winning eBook Collections of scientific, technical and medical ... allow Springer to use its long-term library relationships ...
... U.S. Department of Defense (DOD) has awarded two grants ... high-tech, specialized measuring equipment that can be used in ... of Arizona captured five of the 165 grants awarded ... Sorooshian of the Department of Chemical and Environmental Engineering ...
... is a common perception that bigger, stronger, faster organisms ... with other organisms in a given community. But ... in some structured communities, organisms increase their chances of ... allows competitors to survive as well, a sort of ...
Cached Biology News:UA engineering college lands national support for equipment used in unique research 2Bacteria develop restraint for survival in a rock-paper-scissors community 2
Mouse polyclonal antibody raised against a partial recombinant IFNA13. NCBI Entrez Gene ID = 3447...
Mouse polyclonal antibody raised against a partial recombinant NCLN. NCBI Entrez Gene ID = 56926...
...
Mouse monoclonal antibody raised against a partial recombinant WDR5. NCBI Entrez Gene ID = WDR5...
Biology Products: