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Identification of Insecticides and Herbicides by LC/MS/MS Using the API 2000 LC/MS/MS System

Purpose

Agricultural chemicals typically analyzed by GC/MS can be identified and quantified more effectively and efficiently using LC/MS/MS. These agricultural compounds are extracted from plant materials, and thereby present matrix challenges.

Overview

Health and food safety organizations worldwide are placing great importance on the identification and quantitation of insecticides and herbicides in produce. Chlorinated pesticides have been largely replaced with organophosphorous and organonitrogen pesticides that degrade faster and are typically gentler to the environment.

Until very recently, these compounds had traditionally been analyzed by GC and GC/MS. Separation was often difficult due to the high polarity, low volatility and thermal lability of many pesticides. Difficult matrix effects were usually present since biocide compounds were often extracted from plant material.

Key Features

Triple quadrupole performance in an economical benchtop format

Patented LINAC collision cell prevents crosstalk with shorter multiple reaction monitoring (MRM) dwell times and enhances quantitative accuracy

Curtain Gas interface protects the analyzer from contamination even with crude samples

API 2000 System is a compact, easy to use, triple quadrupole MS system ideal for routine quantitative analysis

Experimental Conditions

The objective of this experiment was to design an LC/MS/MS method to identify and quantify eleven environmentally toxic compounds from two very different classes.

MRM was performed on the API 2000 LC/MS/MS System for enhancing the selectivity and specificity of these low molecular weight compounds.

Compounds of interest for this study include acephate, desethyl
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