Navigation Links
Identification of Insecticides and Herbicides by LC/MS/MS Using the API 2000 LC/MS/MS System

Purpose

Agricultural chemicals typically analyzed by GC/MS can be identified and quantified more effectively and efficiently using LC/MS/MS. These agricultural compounds are extracted from plant materials, and thereby present matrix challenges.

Overview

Health and food safety organizations worldwide are placing great importance on the identification and quantitation of insecticides and herbicides in produce. Chlorinated pesticides have been largely replaced with organophosphorous and organonitrogen pesticides that degrade faster and are typically gentler to the environment.

Until very recently, these compounds had traditionally been analyzed by GC and GC/MS. Separation was often difficult due to the high polarity, low volatility and thermal lability of many pesticides. Difficult matrix effects were usually present since biocide compounds were often extracted from plant material.

Key Features

Triple quadrupole performance in an economical benchtop format

Patented LINAC collision cell prevents crosstalk with shorter multiple reaction monitoring (MRM) dwell times and enhances quantitative accuracy

Curtain Gas interface protects the analyzer from contamination even with crude samples

API 2000 System is a compact, easy to use, triple quadrupole MS system ideal for routine quantitative analysis

Experimental Conditions

The objective of this experiment was to design an LC/MS/MS method to identify and quantify eleven environmentally toxic compounds from two very different classes.

MRM was performed on the API 2000 LC/MS/MS System for enhancing the selectivity and specificity of these low molecular weight compounds.

Compounds of interest for this study include acephate, desethyl atrazine, cyanazine, dichlorvos, dicrotophos, dimethoate, hexazinone, mevinphos, omethoate, phosphamidon, and simazine. Past research experiments have proven acephate and omethoate to be difficult to separate.

Discussion

Calibration curves were established over the entire analyte concentration range of 2 to 15 ng/mL (ppb). Reproducibility (%CV) was exceptionally good considering that results were acquired over an 18-hour period.

For these applications, triple quadrupole mass spectrometry offers advantages over single quadrupole operations since the API 2000 LC/MS/MS System can perform MRM scans which provide superior specificity for the identification of co-eluting chromatographic peaks. Additionally, the API 2000 LC/MS/MS System has the patented LINAC collision cell, which allows for shorter MRM dwell times and increased data confidence during quantitative analysis.

Conclusions

A rapid, sensitive, robust and specific method was developed using the API 2000 LC/MS/MS System. This method separated and quantitated organonitrogen and organophosphorous pesticides in a single chromatographic run and even identified cis- and trans-isomers of particular compounds. Signal-to-noise ratios greater than 5:1 were obtained for all compounds at 1 pg on column while delivering excellent reproducibility. The high sensitivity of this technique allows further dilution if necessary.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. Identification of Differentially Expressed Gene Products with the CastAway System*
2. An Optimal DNA Microarray Substrate for the Identification of Fetal and Somatic Liver Stem Cells of the Rat
3. Identification of Low-Level Oxidation Products in Calmodulin Using NanoSpray Ion Trap Mass Spectrometry
4. Detection and Identification of Phosphorylation Sites in Proteins Using LC/MS/MS with Neutral Fragment Loss Mapping
5. Protect RNA and Improve Target Cell Identification
6. Identification of Disease Causing Mutations in Phenylketonuria by Denaturing Gradient Gel Electrophoresis Using the DCode System
7. Identification of Nonspecific Products Using Melt-Curve Analysis on the iCycler iQ Detection System, Rev A
8. Identification of cells in S phase using the Cell Proliferation Fluorescence Kit and IN Cell Analyzer 1000
9. Identification of Phase I and Phase II Metabolites of Buspirone on the Q TRAP LC/MS/MS System
10. ESR1 Gene Expression in Oncology and Metabolic Diseases Using the ASCENTATM System Target Validation and Identification of Novel Disease Indications
11. Fast Analysis of Phenoxyacetic and Phenoxypropionic Herbicides in Vegetable Matrix by Negative Ion Electrospray LC/MS/MS
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/23/2016)... ... , ... Supplyframe, the Industry Network for electronics hardware design ... Located in Pasadena, Calif., the Design Lab’s mission is to bring together inventors ... and brought to market. , The Design Lab is Supplyframe’s physical representation of ...
(Date:6/23/2016)... ... June 23, 2016 , ... In a new case report published today ... a patient who developed lymphedema after being treated for breast cancer benefitted from an ... paradigm for dealing with this debilitating, frequent side effect of cancer treatment. ...
(Date:6/23/2016)... June 23, 2016  Blueprint Bio, a company dedicated ... the medical community, has closed its Series A funding ... . "We have received a commitment from ... we need to meet our current goals," stated ... the runway to complete validation on the current projects ...
(Date:6/23/2016)... , June 23, 2016 On Wednesday, ... at 4,833.32, down 0.22%; the Dow Jones Industrial Average edged ... closed at 2,085.45, down 0.17%. Stock-Callers.com has initiated coverage on ... ), Nektar Therapeutics (NASDAQ: NKTR ), Aralez Pharmaceuticals ... (NASDAQ: BIND ). Learn more about these stocks ...
Breaking Biology Technology:
(Date:3/31/2016)...   LegacyXChange, Inc. ... LegacyXChange is excited to release its first ... be launched online site for trading 100% guaranteed authentic ... also provide potential shareholders a sense of the value ... industry that is notorious for fraud. The video is ...
(Date:3/23/2016)... Massachusetts , March 23, 2016 /PRNewswire/ ... im Interesse erhöhter Sicherheit Gesichts- und Stimmerkennung ... Xura, Inc. (NASDAQ: MESG ), ... bekannt, dass das Unternehmen mit SpeechPro zusammenarbeitet, ... aus der Finanzdienstleistungsbranche, wird die Möglichkeit angeboten, ...
(Date:3/21/2016)... 2016 Unique technology combines ... superior security   Xura, Inc. ... secure digital communications services, today announced it is working ... enterprise customers, particularly those in the Financial Services Sector, ... authentication within a mobile app, alongside, and in combination ...
Breaking Biology News(10 mins):