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IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!

sed 178 siRNAs from the Silencer Kinase siRNA Library to assess the impact of reducing the expression of individual kinases on cell proliferation and mitotic index (Figure 4). As seen in this example, interfering with the expression of certain kinases has dramatic effects on cell proliferation and the mitotic index, indicating the importance of these kinases in the mammalian cell cycle. This is just one example of the types of experiments possible with libraries of highly effective siRNAs.

Figure 4. Phenotypic Screen Using Silencer Kinase siRNA Library siRNAs. HeLa cells were transfected with individual siRNAs targeting 178 different human kinases. Cell number and mitotic index were measured 48 hours post transfection. The figure depicts results from 92 of the kinase siRNAs and three control siRNAs, shown in black (1=cyclin B1 siRNA, 2=RP42 siRNA, and 3=scrambled control siRNA). The gray shading indicates the normal expected ranges of cell number (top) and mitotic index (bottom).


Library Format
The Silencer Kinase siRNA Library and Silencer GPCR siRNA Library include one nanomole of each siRNA in solution at a concentration of 20 M, providing sufficient material for approximately 400 transfections (100 l transfections at 25 nM siRNA) per siRNA. The siRNAs are arrayed into 96 well plates. For the kinase siRNA library, siRNAs are grouped by kinase functional class, and gene knockdown data is provided for each validated siRNA.

The pre-designed, validated and library siRNA sequences mentioned in this article
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Related biology technology :

1. GENE SILENCING WITH PCR PRODUCTS
2. Silencer Validated siRNAs
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4. Five Ways to Produce siRNAs
5. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
6. Optimizing Chemical Transfection and Electroporation of siRNAs
7. Delivering siRNAs to Difficult Cell Types
8. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
9. Deliver siRNAs Into Primary Cells
10. Optimize Transfection of siRNAs for RNAi
11. RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs
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