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IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!

various concentrations. Forty-eight hours after transfection, RNA from the samples was isolated using the RNAqueous-MAG Total RNA Isolation Kit and target gene expression was measured by real-time RT-PCR. The expression of the target genes in the transfected cells was compared to cells transfected with an equal concentration of the Silencer Negative Control #1. Input cDNA in the different samples was normalized using real-time data for 18S rRNA. The bar graphs represent the average of two data points per siRNA concentration.


Superior Quality, Pre-made siRNAs
Each Silencer Validated siRNA strand is purified by HPLC and subjected to rigorous quality control measures. After annealing, the siRNAs are analyzed by non-denaturing gel electrophoresis to ensure proper annealing, and subjected to rigorous nuclease testing. The result is the highest quality siRNA with a sequence verified to reduce gene expression. Each Silencer Validated siRNA is supplied dried at a unit size of 5 nmol along with nuclease-free water. At press time, over 200 Silencer Validated siRNAs, targeting highly studied human genes, were available.


Silencer siRNA Libraries
Accelerate Pathway Analysis and Target Validation with siRNA Libraries
Although many researchers study genes one at a time, others analyze multiple genes and sometimes entire classes of genes simultaneously. For these researchers, we have combined pre-made Cenix designed and validated siRNAs
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Related biology technology :

1. GENE SILENCING WITH PCR PRODUCTS
2. Silencer Validated siRNAs
3. Cenix-Designed siRNAs for 95% of Human and Mouse Genomes
4. Five Ways to Produce siRNAs
5. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
6. Optimizing Chemical Transfection and Electroporation of siRNAs
7. Delivering siRNAs to Difficult Cell Types
8. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
9. Deliver siRNAs Into Primary Cells
10. Optimize Transfection of siRNAs for RNAi
11. RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs
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