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IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!

various concentrations. Forty-eight hours after transfection, RNA from the samples was isolated using the RNAqueous-MAG Total RNA Isolation Kit and target gene expression was measured by real-time RT-PCR. The expression of the target genes in the transfected cells was compared to cells transfected with an equal concentration of the Silencer Negative Control #1. Input cDNA in the different samples was normalized using real-time data for 18S rRNA. The bar graphs represent the average of two data points per siRNA concentration.


Superior Quality, Pre-made siRNAs
Each Silencer Validated siRNA strand is purified by HPLC and subjected to rigorous quality control measures. After annealing, the siRNAs are analyzed by non-denaturing gel electrophoresis to ensure proper annealing, and subjected to rigorous nuclease testing. The result is the highest quality siRNA with a sequence verified to reduce gene expression. Each Silencer Validated siRNA is supplied dried at a unit size of 5 nmol along with nuclease-free water. At press time, over 200 Silencer Validated siRNAs, targeting highly studied human genes, were available.


Silencer siRNA Libraries
Accelerate Pathway Analysis and Target Validation with siRNA Libraries
Although many researchers study genes one at a time, others analyze multiple genes and sometimes entire classes of genes simultaneously. For these researchers, we have combined pre-made Cenix designed and validated siRNAs
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Related biology technology :

1. GENE SILENCING WITH PCR PRODUCTS
2. Silencer Validated siRNAs
3. Cenix-Designed siRNAs for 95% of Human and Mouse Genomes
4. Five Ways to Produce siRNAs
5. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
6. Optimizing Chemical Transfection and Electroporation of siRNAs
7. Delivering siRNAs to Difficult Cell Types
8. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
9. Deliver siRNAs Into Primary Cells
10. Optimize Transfection of siRNAs for RNAi
11. RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs
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(Date:11/21/2008)...CINNATI, Nov. 21 LCA-Vis... laser vision correction services under the,Lasik...Michael J. Celebrezze,to the position of Chief Fi...ecember 1, 2008. Mr. Celebrezze, who has served a...he LCA-Vision Executive Management team as Senior ...
(Date:11/20/2008)...al impact of NC,s biotechnology sector is $45.8 bi...ARK, N.C., Nov. 20 Nort...s expanding biotechnology enterprise during the pa...s $45 billion, according to data released today at... Norris Tolson, president and CEO of the Biotech...
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...Wis. - Hartford-based api Software, Inc . has en...e management software to four hospitals and health...ool. , ,Founded 1982, api designed the bundled sy...aff recruitment and retention, a key objective in ...ing, for example, has become a buzzword in the nur...
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(Date:11/20/2008)...EDWOOD CITY, Calif., Nov. 19 Digit...ty solutions, today announced DigitalPersona,Pers... for individuals, family,members and business use...ity,protection suite to use biometrics, letting p...ounts -- including email, banking, shopping, and o...
(Date:11/20/2008)... PITTSBURGHResearch from the University of Pittsbu... could finally provide evidence of the first stage...t holds that males and females developed from herm...ompletely understood because the majority of anima...ting separate-sex state too long ago for scientist...
(Date:11/20/2008)... NanoMedical Systems Inc., (NMS), an Austin-based ...University of Texas Health Science Center at Houst...f anti-cancer agents and other medications, has re...rd through the Texas Emerging Technology Fund (ETF...the ETF awards, which were announced by Texas Gov....
(Date:11/20/2008)... This release is available in German . , ... to get a comprehensive overview of which alien sp...sequences for the environment and society. More th...ISIE (Delivering Alien Invasive Species Inventory ...with more than 100 European scientists, funded by ...
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The mini vertical flow workbench is designed to perform PCR protocols in a particle-free environment. The built-in UV light ensures the elimination of any nucleic acid to avoid cross-contamination.
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