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IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!

MALDI-TOF (matrix-assisted laser desorption ionization time-of-flight) mass spectrometry is used to analyze the molecular weight of each RNA oligonucleotide. In addition, all HPLC and PAGE purified oligonucleotides are analyzed for purity by capillary electrophoresis (CE) or HPLC. Finally, each annealed siRNA is analyzed by gel or capillary electrophoresis to confirm that the strands have annealed properly.

See the end of this article for pricing and synthesis options. Complete ordering information is available at www.ambion.com/siRNA.


Silencer Validated siRNAs
Functionally Proven siRNAs
Ambion and Cenix have taken the idea of pre-designed siRNAs one step further with pre-made, Silencer Validated siRNAs. These siRNAs, designed using the Cenix algorithm and targeting important human genes, have been functionally proven to reduce target gene expression by at least 70%. To validate the siRNAs, individual siRNAs were transfected into one or more cell lines at a concentration of 100 nM. mRNA levels of the target gene were assessed by real-time RT-PCR 48 hours later. To be included in this elite product line, the siRNA had to demonstrably reduce mRNA levels by >70%. Most of the Silencer Validated siRNAs reduced target gene expression by 90% or more, which is a good indicator of silencing potency.
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Related biology technology :

1. GENE SILENCING WITH PCR PRODUCTS
2. Silencer Validated siRNAs
3. Cenix-Designed siRNAs for 95% of Human and Mouse Genomes
4. Five Ways to Produce siRNAs
5. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
6. Optimizing Chemical Transfection and Electroporation of siRNAs
7. Delivering siRNAs to Difficult Cell Types
8. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
9. Deliver siRNAs Into Primary Cells
10. Optimize Transfection of siRNAs for RNAi
11. RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs
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