designed siRNAs is Easy
To take advantage of Cenix's design algorithm for your own siRNA experiments, all you need to do is provide the RefSeq number and name of your gene of interest in an easy-to-use online order form. You can now search for your gene of interest in Ambion's extensive online database, which includes all human, mouse, and rat genes for which we have siRNAs pre-designed. If we don't have your gene of interest in our database, or if you have special siRNA design requirements, such as hairpin siRNA template designs, we will be happy to generate these siRNAs on a custom basis (please inquire for pricing). Once your gene is selected, order one, two, or three Cenix designed siRNAs -- the choice is up to you. Then choose the amount of siRNA you need (typically from 20 to 160 nmol) and how you would like it purified (standard, HPLC, or PAGE). We will then synthesize the siRNA and ship it to you, along with the sequence information.

Quality siRNA Synthesis
At Ambion, each siRNA, whether pre-designed by Cenix or designed by you, is synthesized to meet the highest quality standards. Our standard purification procedure includes column purification and yields siRNA that is typically 90% pure (guaranteed >80% pure). HPLC and PAGE purification is also available when higher purity is required. As part of our rigorous quality control procedures,
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1. GENE SILENCING WITH PCR PRODUCTS
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3. Cenix-Designed siRNAs for 95% of Human and Mouse Genomes
4. Five Ways to Produce siRNAs
5. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
6. Optimizing Chemical Transfection and Electroporation of siRNAs
7. Delivering siRNAs to Difficult Cell Types
8. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
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10. Optimize Transfection of siRNAs for RNAi
11. RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs