Navigation Links
Hybridoma Cell Culture Application Using a BioFlo 110 Benchtop Bioreactor

(1) The Biomass monitor and sensor used in this experiment are not available in the marketplace as they are still undergoing test runs.

When this equipment is available, we will make an announcement in the Culture Club, BioFlo 110 newsletter.

The Biomass sensor uses the principle that under the effect of an electric field between two electrodes, live cells (which have intact plasma membranes) polarize and behave as small condensers. The value of the resulting capacitance is measured by the second pair of electrodes and correlates exactly to the concentration of biomass in the culture environment.

In the first stage of culture (0-30 hrs), the pH and DO were controlled by surface aeration at a rate of 200 ml/min. After 30 hours of culture, the capacitance value began to decrease to 0.42 [pf/cm] at 45 hour, as shown in Figure 3. At this time perfusion was started at 1 liter per day, which rapidly increased the value to 0.73 [pf/cm] after 90 min. This phenomenon occurred three times (and is indicated in Figure 4 as T1, T2 and T3) during the S1 and S2 stages.We believe that the plasma membrane polarization for each cell changed, depending on the cell culture conditions.

(2) The equation below was used to calculate the linear correlation between viable cell densities (determined by NucleoCounter, Trypan Blue) and capacitance (determined by biomass probe).

X [10 6 cells/ml] = 1.386 X C [pf/cm]

Here, X is viable cell density [10 6 cells/ml] and C is capacitance [pf/cm]



'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Production of Hybridomas by Electrofusion
2. Production of Hybridomas by Electroporation
3. A Mix-and-Read Cell-Based Assay for Hybridoma Screening Using the FMAT 8100 HTS System
4. Big Blue Rodent Cell Cultures for Mutagenesis Studies
5. Simple Isolation of RNA from Tissue and Cultured Cells
6. The QIAGEN Guide to Animal Cell Culture
7. The QIAGEN Guide to Animal Cell Culture
8. The QIAGEN Guide to Animal Cell Culture
9. The QIAGEN Guide to Animal Cell Culture
10. The QIAGEN Guide to Animal Cell Culture
11. The QIAGEN Guide to Animal Cell Culture
Post Your Comments:
(Date:10/19/2014)... The Asian Automatic patient billing report defines and segments ... revenue. The Automatic patient billing market in Asia is ... at a developing CAGR of 7.2% from 2013 to ... Automatic patient billing market, to get an idea of ... of the segmentation of this market in the same ...
(Date:10/19/2014)... (PRWEB) October 19, 2014 The report ... Market Share, Trade, Prices, Geography Trend and Forecast (2011 ... sales category, and geography and studies the major market ... North America, Europe, and Asia. , The global chocolate ... 2010.to $98.3 billion in 2016 at an estimated CAGR ...
(Date:10/19/2014)... The report "Unmanned Ground Vehicle Market ... Payload, Type & by Geography - Forecasts & ... ground vehicle market into various sub segments with ... The report also identifies the factors driving this ... with the adoption trends. , The unmanned ground ...
(Date:10/18/2014)... The “Human Insulin Market- by Product Type, [Human ... Insulin (Rapid Acting, Long Acting, Premixed)], by Brand ... Forecast to 2018” provides a detailed overview of ... trends, and strategies impacting the global human insulin ... the revenue and share analysis. , The global ...
Breaking Biology Technology:The Asian Automatic patient billing market is estimated to grow to around $463.9 million by 2018 - New Report by MicroMarket Monitor 2The Asian Automatic patient billing market is estimated to grow to around $463.9 million by 2018 - New Report by MicroMarket Monitor 3Chocolate Market Projected To Reach $98.3 Billion by 2016 - New Report by MarketsandMarkets 2Chocolate Market Projected To Reach $98.3 Billion by 2016 - New Report by MarketsandMarkets 3Chocolate Market Projected To Reach $98.3 Billion by 2016 - New Report by MarketsandMarkets 4Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 2Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 3Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 4Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 5Medical Human Insulin Market Is Expected to Reach $42 billion by 2019 - New Research Report by MarketsandMarkets 2Medical Human Insulin Market Is Expected to Reach $42 billion by 2019 - New Research Report by MarketsandMarkets 3Medical Human Insulin Market Is Expected to Reach $42 billion by 2019 - New Research Report by MarketsandMarkets 4Medical Human Insulin Market Is Expected to Reach $42 billion by 2019 - New Research Report by MarketsandMarkets 5
... to learn if your water source has hexavalent chromium ... request a water quality report, said NJIT Professor Taha ... engineers should be able to provide such a report ... levels may also be available. "In general, hexavalent ...
... Princeton University engineers have developed a new laser sensing ... from a distance and scientists to better measure airborne ... to send a laser pulse out and get another ... a professor of mechanical and aerospace engineering at Princeton, ...
... stroke and head trauma is due to damage caused the ... this damage has been impossible, until now, as many drugs ... often don,t work as intended. But a method originally devised ... offer a way to avert such glutamate-induced harm. ...
Cached Biology Technology:Removal of hexavalent chromium from your drinking water 2Removal of hexavalent chromium from your drinking water 3'Air laser' may sniff bombs, pollutants from a distance 2'Air laser' may sniff bombs, pollutants from a distance 3Hope for stroke victims 2
(Date:10/15/2014)... 15, 2014 Sandata Technologies, LLC, an ... community-based care, today announced it released a case ... Electronic Visit Verification™ Solution (EVV™)  for Quality Care ... health company founded in 1996 and has five ... The study details the challenges facing ...
(Date:10/15/2014)... pneumonia in female mice to an enzyme activated by ... more resistant to respiratory infections than males. Now, an ... to bacterial pneumonia in female mice is linked to ... show that this enzyme is ultimately activated by the ... team, lead by Professor Lester Kobzik at the Harvard ...
(Date:10/14/2014)... A new study published in Cancer Research ... liver and colon cancers—can promote the development of skin ... proliferation and survival of sun-damaged skin cells. , Previously ... seven proteins called sirtuins that help regulate genomic stability ... aging. SIRT6 helps repair DNA damage, which can lead ...
Breaking Biology News(10 mins):Sandata Announces Case Study with Quality Care Services, Inc. 2Two-faced gene: SIRT6 prevents some cancers but promotes sun-induced skin cancer 2
... PhD, testified on behalf of the American Society of ... Commerce, Justice, Science, and Related Agencies. Hood, who ... in support of funding for the National Science Foundation ... 2012. While recognizing the difficult fiscal environment faced ...
... is available in French . , Soon, drug ... surrounding tissue to the medication,s toxic effects will no longer ... the work of Professor Sylvain Martel, Director of the Nanorobotics ... world,s first researcher to have guided a magnetic sphere through ...
... in Spanish . A U.S. Department ... that seems just right for today,s intensive rotational grazing. ... Casler, an Agricultural Research Service (ARS) geneticist at the agency,s ... the grass as meadow fescue. Meadow fescue has been long ...
Cached Biology News:ASPB member testifies in support of National Science Foundation 2World first -- Localized delivery of an anti-cancer drug by remote-controlled microcarriers 2Dairy farmer finds unusual forage grass 2
... For laboratories working in a ... the only pipette calibration software that ... keeping within a framework that meets ... Pro is a secure solution that ...
... are focused on sequencing, analysis of proteins ... areas of genomics and proteomics, EvoQuest ™ ... your research and manufacturing challenges. Invitrogen scientists ... project from beginning to end in a ...
... 17 amino acid synthetic peptide whose sequence is from rat SCAMP ... terminus): C - N(217) - Q - P - Q ... T - P - N - Y - T - Y ... for neutralization and control experiments with the polyclonal antibody that reacts ...
... has over 23 years of experience in ... have made antibodies to large and small ... antigens, and plasmid DNA-encoded antigens. We understand ... so our hybridoma development programs are never ...
Biology Products: