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Hybridoma Cell Culture Application Using a BioFlo 110 Benchtop Bioreactor

change the default Fermentation application to Cell Culture application.

For further details refer to the Operation Section in the BioFlo 110 manuals.

Control Setpoints
Setpoints were keyed into the controller prior to inoculation. The vessel was allowed to equilibrate prior to inoculation. The DO loops Current Value remained higher than setpoint until the culture was introduced.
Temperature . . . . . . . . . . . . . ... .37C
pH . . . . . . . . . . . . . . . . . . . . . . . .7.2
DO . . . . . . . . . . . . . . . . . . . . . .. .50%
Agitation . . . . . . . . . . . . . . . . .. .70-130 rpm
O2 (Gases) . . . . . . . . . . . . . . . .. .4 Gas

PH Control
The pH probe was calibrated prior to the autoclave cycle (refer to BioFlo 110 Instruction Manual). CO2 gas and a liquid base were used to maintain the pH setpoint. pH control parameters were:
Base . . . . . . . . . . . .Sodium bicarbonate, 8% solution
Pump . . . . . . . .. . . .Pump 4 of the 4-Pump Module
Transfer tubing . .. .Narrow bore silicone tubing, as supplied (116" ID & 316" OD - NBS Catalog # P0740-2396)
Vessel inlet . . . .. . . .Triport adapter in the vessel headplate

PCU setup: (pH loop)
Pump 4 plugged into "BASE" Power Controller Outlet
pH Control Selections:
Auto mode
Multiplier = 100% before inoculation
Multiplier = 25% after inoculation
Dead-band = 0.1
PID values: factory defaults

Dissolved Oxygen (DO) Control
The DO probe was calibrated after the autoclave cycle (ref
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