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Hybridoma Cell Culture Application Using a BioFlo 110 Benchtop Bioreactor

we substituted an optional NBS spin filter impeller for the standard pitched blade impeller. The spin filter impeller is very useful for a continuous perfusion process because it is a simple device that lends itself to media exchange and can be easily scaled up. With most commonly available spin filters, screens rapidly become clogged when cells reach high cell density during perfusion. NBS, however, has designed spin filters with three different screen sizes that are suitable for most cell types. Feel free to contact NBS for screen size suggestions.




Control System
Listed below are six control modules included in an Advanced Cell Culture Kit, which were also used for this application:


Materials and Methods

Overview

Sterilize the vessel with phosphate buffer solution (PBS) for 60 minutes.
Remove the PBS from the vessel.
Add 1.5 L of media into the vessel.
Key in control setpoints.
Inoculate 500 ml of inoculum suspension to provide a starting cell count of 2.44 x 105 cells/ml.
Use BioCommand software to automatically calculate and control glucose addition throughout the run.

Medium
RPM1640 medium from Invitrogen, supplemented with 5% FBS from Hyclone and 0.1% Pluronic from Gibco.

Inoculum
Hybridoma cell line 3G5 from ATCC
Inoculum was cultivated in an NBS Shaker (Innova 2000), which was inside a CO2 Incubator (Innova CO- 170 NBS Catalog # M1305-0000)

PCU: Select the Application
On the initial set-up screen, the USE selector button will allow you to
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