Navigation Links
Hybridoma Cell Culture Application Using a BioFlo 110 Benchtop Bioreactor


Introduction
This Application Report is one in a series documenting simple protocols for culturing cells in a BioFlo 110 benchtop fermentor/bioreactor system. The modular BioFlo 110 is offered with an extensive choice of vessels, impellers and control modules to enable growth of a wide variety of cultures, including mammalian, insect, plant, yeast and bacterial cells. Complete kits are also available. This report concentrates on achieving highdensity hybridoma cell cultures using NBS Advanced Cell Culture Kit.

For this application, researchers in NBS cell culture laboratory used a standard 3.0L BioFlo 110 Advanced Cell Culture Kit with a magnetic drive and a water-jacketed vessel (NBS Catalog # M1273-1212). A suspension cell spin filter, with a 12-14 micron screen (NBS Catalog # M1273-3202) was used for perfusion in order to grow the hybridoma cells in a continuous mode. Additionally, BioCommand Plus supervisory software (NBS Catalog # M1291-0000) was used to control the feeding schedule and collect data; and a NucleoCounter (NBS Catalog # M1293-0000) was used to count viable cells.


The Bioreactor

Vessel

This BioFlo 110 Advanced Cell Culture Kit was equipped with a magnetic drive and water-jacketed 3.0 L vessel with a nominal working volume of 2.25 L. All BioFlo 110 Advanced Cell Culture Kits are configured with six control modules, a complete vessel assembly including a pitched blade agitation impeller, foam/level sensors, as well as dissolved oxygen and pH probes (Mettler Toledo), three liquid addition bottles, cables, tubing, clamps and other related items. Optional direct drive and magnetic-drive agitation systems, as well heat-blanketed vessel options, are available.

For this application
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Production of Hybridomas by Electrofusion
2. Production of Hybridomas by Electroporation
3. A Mix-and-Read Cell-Based Assay for Hybridoma Screening Using the FMAT 8100 HTS System
4. Big Blue Rodent Cell Cultures for Mutagenesis Studies
5. Simple Isolation of RNA from Tissue and Cultured Cells
6. The QIAGEN Guide to Animal Cell Culture
7. The QIAGEN Guide to Animal Cell Culture
8. The QIAGEN Guide to Animal Cell Culture
9. The QIAGEN Guide to Animal Cell Culture
10. The QIAGEN Guide to Animal Cell Culture
11. The QIAGEN Guide to Animal Cell Culture
Post Your Comments:
(Date:12/24/2014)... GMO corn cases filed across the ... in the process of being consolidated in a Kansas ... In Re: Syngenta AG MIR 162 Corn Litigation, MDL ... District of Kansas. , Management of the Syngenta GMO ... U.S. District Judge John W. Lungstrum.* , As a ...
(Date:12/24/2014)... December 23, 2014 According to Ross Selinger, ... Affiliate of ITRA Global, the national office market continues to ... This is evidenced by the third quarter’s surprising 3.9% ... Low energy costs have held inflation down and the ... is the housing market remaining soft. , This solid economic ...
(Date:12/24/2014)... Diego, CA (PRWEB) December 23, 2014 ... where their Twitter followers can submit their #HolidayInTheLab ... themselves and their lab mates. , For those struggling ... lab mates, the Pipette.com Holiday in the Lab Contest ... Pipette.com Twitter followers to send pictures of ...
(Date:12/24/2014)... 2014 Sigma-Aldrich Corporation (NASDAQ: ... under the Hart-Scott-Rodino Antitrust Improvements Act (HSR Act) ... 22, 2014, thereby completing the U.S. HSR Act ... of the Company by Merck KGaA, Darmstadt, Germany.  ... condition to closing the transaction, which remains subject ...
Breaking Biology Technology:Carey Danis & Lowe Reports on Syngenta GMO Corn Transfer Order 2ITRA Global Reports Long Island Is Out of Sync With The National Office Market 2ITRA Global Reports Long Island Is Out of Sync With The National Office Market 3Major Pipette Distributor Pipette.com Announces their Holiday in the Lab Contest 2Acquisition of Sigma-Aldrich by Merck KGaA, Darmstadt, Germany, Receives U.S. Antitrust Clearance 2Acquisition of Sigma-Aldrich by Merck KGaA, Darmstadt, Germany, Receives U.S. Antitrust Clearance 3
... Recognized for Development of Statistical Methods and SoftwareSAN DIEGO, ... and of Health Research & Policy at Stanford ... Academy of Sciences. Election to the National Academy ... can be accorded to a scientist in the US ...
... published,today in the online edition of Nature Genetics, scientists ... from Iceland, Denmark and the,Netherlands present the discovery of ... (SNPs) that influence the age of girls at menarche, ... by both genetic,and environmental factors, though the SNPs reported ...
... naturally occurring elements, add another to the list of those ... physics in Arts & Sciences at Washington University in St. ... the time discovered that europium becomes superconducting at 1.8 ... making it the 53rd known elemental superconductor and the 23rd ...
Cached Biology Technology:Professor Wing Wong Elected to the National Academy of Sciences 2deCODE Study Shows Complex Interplay of the Human Biological Clock With Height, Weight and Disease 2deCODE Study Shows Complex Interplay of the Human Biological Clock With Height, Weight and Disease 3deCODE Study Shows Complex Interplay of the Human Biological Clock With Height, Weight and Disease 4Europium discovery 2Europium discovery 3
(Date:12/22/2014)... 22, 2014 Research and Markets ... addition of the "The Global Watermarking ... offering. http://photos.prnewswire.com/prnh/20130307/600769 ... overview of the global digital media watermarking ... and monitor piracy by securely and invisibly ...
(Date:12/19/2014)... 2014   LaunchKey , the first decentralized mobile ... Internet of Things era, today announced the close of ... was led by Metamorphic Ventures with participation from ENIAC ... and others.  LaunchKey has raised $4 million to date, ... team and bring LaunchKey to market in 2015. ...
(Date:12/17/2014)... , Dec. 16, 2014 Research and Markets ... addition of the "Global Chemical Sensor Market ... http://photos.prnewswire.com/prnh/20130307/600769 One ... increasing demand for medical sensors in biomedical applications. ... for quick and correct diagnosis during surgical procedures. ...
Breaking Biology News(10 mins):The Global Watermarking and Fingerprinting Markets 2LaunchKey Raises $3 Million in Additional Funding Led by Metamorphic Ventures 2LaunchKey Raises $3 Million in Additional Funding Led by Metamorphic Ventures 3Global Chemical Sensor Market 2015-2019: Key Vendors are Abbott Laboratories, Bayer, Hoffmann La-Roche, Johnson & Johnson, NGK Spark Plugs and Robert Bosch 2
... FORT COLLINS - In the last 50 years, modern ... of today,s veterinary and human medicine students still hone ... pig,s feet before transitioning to a live patient. An ... artificial body parts that look, feel, behave, and even ...
... been at least a billion years since plants and ... eons shared a common threat in the form of ... in remarkably similar mechanisms for detecting the molecular signatures ... treatment of infectious diseases in humans. The recognition ...
... produce many antibodies against the protein encapsulating the virus, most ... A new study suggests why some of the most common ... a form it takes after the virus has already invaded ... Hospital Boston and their colleagues. The findings, published ...
Cached Biology News:Invention helps students learn surgical techniques before operating on patients 2Reading the signs: Plants and animals found common ground in response to microbial threats 2Designing more effective anti-HIV antibodies 2Designing more effective anti-HIV antibodies 3