Navigation Links
Human Universal cDNA Library Array I

ing procedure (Figure 4).

Figure 4

Screening for Human b-actin Clones

The presence of the ubiquitous b-actin gene (1.8 kb)4 was analyzed in each library to evaluate the efficiency of the fractionation-normalization procedures. In this analysis, we probed the libraries with two different radiolabeled oligonucleotides.5 One probe was designed to hybridize to the beginning of the 5 translated region (ATG included) while the other corresponded to the end of the 3 untranslated region (Figure 5 and Figure 6). The percentage of full-length cDNAs was determined by comparing the two results.

Figure 5

In situ hybridization of 6,000 colonies from the library obtained before fractionation established that b-actin was originally represented at approximately 1 in 200 clones with 45% being full-length (data not shown). Dot blot analysis on the Master Membranes (Figure 6) showed that after fractionation and one round of normalization, the representation of this gene fell to 1 in 5,000 clones with 70% being full length. After the second round of normalization no b-actin clone was detected in the organized colonies, suggesting that, after extensive hybridization, each of the 22,000 clones arrayed could be unique.

Stratagene Now Provides These Membranes for Screening Procedures

Figure 6

A set of four Master Membranes, each the size of a microwell plate, allows the screening of 1,536 pools of 96 clones in the same conditions as ordinarily
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Human Primary Preadipocytes and Differentiated Adipocytes
2. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
3. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
4. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
5. Map and Link Human Genetic Disorders with SSLP Analysis
6. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
9. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
10. Sequence-Validated and Expression-Tested Human cDNA in a Dual Expression Vector
11. Stratagenes Human cDNA Microarrays: Perform Gene Expression Profiling and Discover New Genes
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Human Universal cDNA Library Array

(Date:3/30/2015)... England , March 30, 2015 ... developing novel biological drugs to treat cancer, viral ... of Professor Sir John Bell , the ... Chairman of the Office for the Strategic Coordination ... with immediate effect.      (Photo: ...
(Date:3/29/2015)... , March 29, 2015  Caris Life ... data in small cell cervical cancer (SCCC) ... Molecular Intelligence®, the company,s panomic, comprehensive tumor ... mutations which may explain sensitivities and resistance ... identification of certain biomarkers may guide treatment ...
(Date:3/27/2015)... , 27. März 2015  Peter Walter ... Biomedical Science für seine bahnbrechende Arbeit ausgewählt, ... Zellkompartimenten transportiert werden, sowie für die Entschlüsselung ... Reaktion auf Stress nutzen, der mit der ... ist Professor für Biochemie an der University ...
(Date:3/27/2015)... SHANGHAI , March 27, 2015 ... a leading open-access R&D capability and technology platform ... device industries, announced today that an Investigational New ... monoclonal antibody for rheumatoid arthritis has been accepted ... Administration (CFDA).  In September 2012, ...
Breaking Biology Technology:Immunocore Appoints Professor Sir John Bell to its Board of Directors 2Immunocore Appoints Professor Sir John Bell to its Board of Directors 3Immunocore Appoints Professor Sir John Bell to its Board of Directors 4Immunocore Appoints Professor Sir John Bell to its Board of Directors 5Study Using Caris Molecular Intelligence Multiplatform Tumor Profiling Identifies Potential Treatment Strategies for Small Cell Cervical Cancer 2Study Using Caris Molecular Intelligence Multiplatform Tumor Profiling Identifies Potential Treatment Strategies for Small Cell Cervical Cancer 3Deutschstämmiger Zellbiologe Peter Walter gewinnt den Vilcek Prize in Biomedical Science 2015 2Deutschstämmiger Zellbiologe Peter Walter gewinnt den Vilcek Prize in Biomedical Science 2015 3Deutschstämmiger Zellbiologe Peter Walter gewinnt den Vilcek Prize in Biomedical Science 2015 4Investigational New Drug Application for WuXi MedImmune's Monoclonal Antibody Accepted for Review by CFDA 2Investigational New Drug Application for WuXi MedImmune's Monoclonal Antibody Accepted for Review by CFDA 3
... Breweries announced a new partnership, which will create a joint venture ... , ... Lexington, KY (Vocus) March 11, 2009 -- ... announced a new partnership, which will create a joint venture company ...
... Legislation as Pro-Patient and Pro-Pharmaceutical InnovationARLINGTON, Va., ... Pharmaceutical Association (GPhA) released the following statement ... Jaeger regarding the introduction of H.R. 1427, ... and Access to Life-Saving Medicine Act.""For the ...
... for rural Indians - Leverages Satyam,s earlier success with ... (NYSE: SAY ) a leading global consulting ... was honored, along with the LV Prasad Eye Institute, ... program, which provides expert and affordable eye care to ...
Cached Biology Technology:Alltech, Mitsui and Asahi Breweries Create New Venture 2Alltech, Mitsui and Asahi Breweries Create New Venture 3GPhA Statement on the Introduction of the Bipartisan Biogenerics Consensus Bill: 'Promoting Innovation and Access to Life-Saving Medicine Act' 2Satyam Wins Consumer Health World Award for Tele-Ophthalmology Program 2Satyam Wins Consumer Health World Award for Tele-Ophthalmology Program 3Satyam Wins Consumer Health World Award for Tele-Ophthalmology Program 4
(Date:3/12/2015)... Va. , March 12, 2015 IriTech, ... and a member of the Texas Instruments Design Network, ... Naidu has demonstrated its IriShield USB MK2120U device ... scanning facility for pension distribution. The ... assembled to be marketed in India ...
(Date:3/11/2015)... and Markets ( http://www.researchandmarkets.com/research/3p32qm/access_control ) has announced ... Product, Application & By Geography - Global Forecast & ... This report predicts that the access control ... with an estimated CAGR of 10.6%. The ... as contact cards & readers, contactless cards & readers, ...
(Date:3/10/2015)... 2015   Tute Genomics , pioneer in affordable, ... next-generation sequencing company PrimBio Research Institute to ... gene panel interpretation. PrimBio, a certified Life ... current technologies to support the work of experts on ... two types of exome sequencing services: 1) Targeted Exome ...
Breaking Biology News(10 mins):Indian States See IriTech's Scanner Bolstering Efficiency of Authentication for Aadhaar 2Indian States See IriTech's Scanner Bolstering Efficiency of Authentication for Aadhaar 3Global Access Control (Cards & Readers, Biometrics, Controllers, Management Software) Market 2015-2020 - Analysis of the $10 Billion Market 2PrimBio Research Selects Tute as Whole Exome Analysis Provider, Creating Integrated, End-to-End Solution for Next-Generation Sequencing & Data Analysis 2PrimBio Research Selects Tute as Whole Exome Analysis Provider, Creating Integrated, End-to-End Solution for Next-Generation Sequencing & Data Analysis 3
... E or a combination of the supplements had a similar ... supplements, according to data from a randomized controlled trial in ... the National Cancer Institute. Epidemiological studies have suggested ... and thus antioxidants, may have a lower risk of cancer. ...
... has the honour of becoming the first student from ... competition that pitted engineering students and young professionals from ... the Institution of Engineering and Technology (IET), UK, the ... Mechanical & Aerospace Engineering beat three other contestants in ...
... Is Sensitive to Irradiation Researchers led by ... irradiation damages fat tissue. They report their findings in ... of Pathology . Radiation therapy directed ... of tissues such as fat tissue has often been ...
Cached Biology News:Vitamins C and E and beta carotene again fail to reduce cancer risk in randomized controlled trial 2NTU undergrad is first Singapore champion of an internationally acclaimed engineering competition 2NTU undergrad is first Singapore champion of an internationally acclaimed engineering competition 3Tips from the American Journal of Pathology 2Tips from the American Journal of Pathology 3Tips from the American Journal of Pathology 4Tips from the American Journal of Pathology 5
Purified anti-PI3K p110 delta...
... Tyrosine Hydroxylase (Ser40) Polyclonal Antibody ... against synthetic phosphopeptide corresponding to ... phospho-Ser40 of rat Tyrosine Hydroxylase ... by sequential chromatography on phospho- ...
NF kappa B Related Sampler Kit 10g each...
... Ambions MAXIscript In Vitro Transcription Kits synthesize ... x 10 9 cpm/g. High specific ... ribonuclease protection assays, in situ hybridization, and ... used for the synthesis of modest amounts ...
Biology Products: