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Human Primary Preadipocytes and Differentiated Adipocytes

as norepinephrine or isoproterenol, to induce this metabolic pathway. The lipolysis response can be assayed by measuring the release of glycerol into the culture medium (Figure 4). Thus, these cells are useful for analyzing compounds or genes that induce, or are involved with, this important function of the mature adipocyte.

Figure 4

Conclusions

With Stratagenes new Human Predifferentiated Adipocytes and Human Differentiated Adipocytes, researchers have a new class of reagents with which to study human adipose biology. It is now possible to directly study the factors that affect human adipose differentiation, and to identify agents which may promote or interfere with the progression of obesity. Gene expression in both adipocytes and preadipocytes can be achieved with the aid of Primary Transfection ENHANCER, a new reagent developed by Stratagene to enhance primary cell transfection.

REFERENCES
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  3. Arch, J.R.S. and Wilson, S. (1996) Int. J. Obes. 20: 191-199.

  4. Claffey, K. and Halvorsen, Y.C. unpublished results.

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  6. Lehmann, J.M., et al. (1995) J. Clin. Invest. 270: 12953-12956.

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