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Human Primary Preadipocytes and Differentiated Adipocytes

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By characterizing gene expression and regulation, we may unravel the mysteries of the adipocytefrom its role as an endocrine receptor to its role as a mediator of energy homeostasis. Transfection experiments, which lead to overexpression of wild type or mutated protein forms, are used to characterize many gene functions. The phenotype of the altered expression state allows the effects of single gene changes to be examined, which may model metabolic derangement associated with particular diseases.9

To date we have successfully transfected Human Differentiated Adipocytes with the human adipose- specific obese gene and peroxisome proliferator activated receptor gamma (PPARg) gene promoter-driven reporters (data not shown). In experiments to improve the transfectability of Human Differentiated Adipocytes or Human Predifferentiated Adipocytes using Stratagenes Transfection MBS Mammalian Transfection Kit or LipoTAXI Transfection Reagent, respectively, we showed that including the Primary Transfection ENHANCER results in a higher transfection efficiency (Figure 3, panel A and panel B).

Figure 3

Lipolysis: A Differentiated Adipocyte Process

One of the key functions of adipocytes is to adjust lipid storage according to the physiological energy needs of the organism. When extra calories are required, adipocyte intracellular triglyceride stores are metabolized to glycerol and fatty acids for further oxidation, a process termed lipolysis.10 Our Human Differentiated Adipocytes respond to adrenergic hormone receptor agonists, such
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