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Human Primary Preadipocytes and Differentiated Adipocytes

for total differentiation potential, as measured by lipid accumulation.

Up to 90% of the Human Predifferentiated Adipocytes can be induced to differentiate after they have been treated with a hormone and chemical cocktail. This treatment promotes a cascade of transcription factors activation and adipocyte-specific gene expression that leads to the mature adipocyte phenotype (Figure 1, panel A and B). Predifferentiated Adipocyte Differentiation Medium is also available from Stratagene as a separate reagent.

Figure 1

During the differentiation process5, key adipocyte- specific genes, such as PPARg, fatty acid binding protein, and obese gene, are expressed.6,7,8 Expression of signaling molecules, such as leptin, the obese gene product is induced during differentiation (Figure 2).

Figure 2

Practical Applications

By using Stratagenes Human Predifferentiated Adipocytes, various questions can be addressed: What makes these cells differentiate from fibroblast-like cells to adipocytes? What are adipocytes similarities and differences? What chemical and endocrine effectors transform preadipocytes? What gene expression changes are necessary and/or sufficient for preadipocyte transformation?

Human Predifferentiated Adipocytes are available in a 24- or 96-well format and are shipped ready to use. Besides providing a unique tool to study the processes that trigger and promote differentiation, these cells can be used to identify agents that block or slow the differentiation process.

Human Adipocyte-Specific Gene Expression'"/>

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