( for total differentiation potential ...)Human Primary Preadipocytes and Differentiated Adipocytes

Up to 90% of the Human Predifferentiated Adipocytes can be induced to differentiate after they have been treated with a hormone and chemical cocktail. This treatment promotes a cascade of transcription factors activation and adipocyte-specific gene expression that leads to the mature adipocyte phenotype (Figure 1, panel A and B). Predifferentiated Adipocyte Differentiation Medium is also available from Stratagene as a separate reagent.

Figure 1

During the differentiation process⁵, key adipocyte- specific genes, such as PPARg, fatty acid binding protein, and obese gene, are expressed.^6,7,8 Expression of signaling molecules, such as leptin, the obese gene product is induced during differentiation (Figure 2).

Figure 2

Practical Applications

By using Stratagenes Human Predifferentiated Adipocytes, various questions can be addressed: What makes these cells differentiate from fibroblast-like cells to adipocytes? What are adipocytes similarities and differences? What chemical and endocrine effectors transform preadipocytes? What gene expression changes are necessary and/or sufficient for preadipocyte transformation?

Human Predifferentiated Adipocytes are available in a 24- or 96-well format and are shipped ready to use. Besides providing a unique tool to study the processes that trigger and promote differentiation, these cells can be used to identify agents that block or slow the differentiation process.

Human Adipocyte-Specific Gene Expression'"/>

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