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How to select the best spectrofluorometer

equals the square root of the measured number1,

Quality of electronics does not matter: without 10 000 photons, the accuracy cannot be 1%. (Analog detection has other problems, e.g., hidden noise in the signal, and response times that skew the data.) Hence, an instrument with twice the S/N is twice as accurate under the same conditions.


Where does sensitivity come from?
If everyone needs sensitivity, why doesnt everyone have it? Its a question of economics and quality. Photon-counting electronics, reflective (mirror-based) rather than refractive (lensbased) optics, planar instead of concave gratings, full instrument automation, and all the other high-quality components of a SPEX spectrofluorometer are more expensive. Thus, an accurate, time-saving, sensitive instrument is worth more than one that is not.


How much is your research worth?


1 Strobel, H.A. and Heineman, W.R., Chemical Instrumentation: A Systematic Approach, 3rd ed., Wiley, New York, 1989, p. 345.


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