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HotStarTaq PCR Handbook

2, 25 mM: Conductivity, pH, sterility, and performance in PCR are tested. Distilled water: Conductivity, pH, sterility, and performance in PCR are tested. Endonuclease, exonuclease, and RNase activities are tested. HotStarTaq Master Mix Kit: PCR reproducibility assay: The PCR reproducibility assay described above is performed in parallel using HotStarTaq Master Mix and using the separate reagents with the same lot numbers.

PCR Protocol Using HotStarTaq DNA Polymerase

This protocol serves only as a guideline for PCR amplification. Optimal reaction conditions such as incubation times, temperatures, and amount of template DNA may vary and must be individually determined.

Notes:

HotStarTaq DNA Polymerase requires an activation step of 15 min at 95C (see step 6 of this protocol).
Set up all reaction mixtures in an area separate from that used for DNA preparation or PCR product analysis.
Use disposable tips containing hydrophobic filters to minimize cross-contamination.
If required, prepare a dNTP mix containing 10 mM of each dNTP. Store this mix in aliquots at 20C.


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