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HotStarTaq PCR Handbook

ed under Exo. Endonuclease activity assay: Plasmid DNA is incubated with HotStarTaq DNA Polymerase in PCR Buffer. Endonuclease activity per unit
of enzyme is indicated under Endo. RNase activity assay: RNA is incubated with HotStarTaq DNA Polymerase in PCR Buffer. RNase activity per unit of enzyme is indicated under RNase. RNase activity assay: RNA is incubated with HotStarTaq DNA Polymerase in PCR Buffer. RNase activity per unit of enzyme is indicated under RNase. Protease activity assay: HotStarTaq DNA Polymerase is incubated in storage buffer. Protease activity per unit of enzyme is indicated
under Protease. Self-priming activity assay: Assays are performed under standard PCR conditions, without primers, using HotStarTaq DNA Polymerase and human genomic DNA (purified with the QIAamp DNA Blood Mini Kit). The absence of PCR product is indicated by No under Self-priming. Buffers and Reagents: PCR Buffer, 10x: Conductivity, pH, sterility, and performance in PCR are tested. Q-Solution, 5x: Conductivity, pH, sterility, and performance in PCR are tested. MgCl
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