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HotStarTaq PCR Handbook

ion rate: 5x concentrated Half-life: Ultrapure quality, PCR-grade 5'3' exonuclease activity: Yes Extra A addition: Yes 3'5' exonuclease activity: No Nuclease contamination: No Protease contamination: No RNase contamination: No Self-priming activity: No Storage and dilution buffer: 20 mM TrisCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% (v/v) Nonidet P-40, 0.5% (v/v) Tween 20, 50% glycerol (v/v), stabilizer; pH 9.0 (20C) Buffers and reagents: PCR Buffer: 10x concentrated. Contains TrisCl, KCl, (NH4)2SO4, 15 mM MgCl2; pH 8.7 (20C). Q-Solution: 5x concentrated MgCl2 solution: 25 mM HotStarTaq Master Mix: 2x concentrated. Contains HotStarTaq DNA Polymerase, PCR Buffer
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