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HotStarTaq PCR Handbook

Q-Solution

The HotStarTaq DNA Polymerase Kit is provided with Q-Solution, an innovative PCR additive that facilitates amplification of difficult templates by modifying the melting behavior of DNA. This unique reagent will often enable or improve a suboptimal PCR caused by templates that have a high degree of secondary structure or that are GC-rich. Unlike other commonly used PCR additives such as DMSO, Q-Solution is used at just one working concentration, it is nontoxic, and PCR purity is guaranteed. For further information, please read the PCR Protocol Using HotStarTaq DNA Polymerase and Q-Solution.

Product Specifications


Enzyme:
HotStarTaq DNA Polymerase is a modified form of a recombinant 94-kDa DNA polymerase, originally isolated from Thermus aquaticus, cloned in E. coli. (Deoxynucleosidetriphosphate: DNA deoxynucleotidyltransferase, EC 2.7.7.7). One unit of HotStarTaq DNA Polymerase is defined as the amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material within 30 min at 72C, under the assay conditions described in the section Quality Control on the following page.

Concentration: 5 units/l Substrate analogs: dNTP, ddNTP, dUTP, biotin-11-dUTP, DIG-11-dUTP, fluorescent dNTP/ddNTP dNTP Mix: 24 kb/min at 72C Extens
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