The HotStarTaq DNA Polymerase Kit is provided with Q-Solution, an
innovative PCR additive that facilitates amplification of difficult
templates by modifying the melting behavior of DNA. This unique reagent
will often enable or improve a suboptimal PCR caused by templates
that have a high degree of secondary structure or that are GC-rich.
Unlike other commonly used PCR additives such as DMSO, Q-Solution
is used at just one working concentration, it is nontoxic, and PCR
purity is guaranteed. For further information, please read the PCR
Protocol Using HotStarTaq DNA Polymerase and Q-Solution.
HotStarTaq DNA Polymerase is a modified form of a recombinant 94-kDa
DNA polymerase, originally isolated from Thermus aquaticus, cloned
in E. coli. (Deoxynucleosidetriphosphate: DNA deoxynucleotidyltransferase,
EC 18.104.22.168). One unit of HotStarTaq DNA Polymerase is defined as the
amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble
material within 30 min at 72C, under the assay conditions described
in the section Quality Control on the following page.
dNTP, ddNTP, dUTP, biotin-11-dUTP, DIG-11-dUTP,
24 kb/min at 72C
Source:Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 Related biology technology :1
. HotStarTaq DNA Polymerase2
. QIAGEN Multiplex PCR Handbook3
. TransMessenger Transfection Reagent Handbook4
. Transfection Reagent Selector Kit Handbook