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HotStarTaq PCR Handbook

. HotStarTaq DNA Polymerase makes hot-start PCR simple and easy, eliminating the extra handling steps and contamination risks associated with conventional hot-start methods.

HotStarTaq DNA Polymerase

HotStarTaq DNA Polymerase is a modified form of the recombinant 94 kDa Taq DNA Polymerase from QIAGEN. HotStarTaq DNA Polymerase is provided in an inactive state with no polymerase activity at ambient temperatures. This prevents the formation of misprimed products and primerdimers at low temperatures. HotStarTaq DNA Polymerase is activated by a 15-minute, 95C incubation step, which can easily be incorporated into existing thermal cycling programs. HotStarTaq DNA Polymerase provides high PCR specificity and often increases the yield of the specific PCR product. PCR setup is quick and convenient as all reaction components can be combined at room temperature.

QIAGEN PCR Buffer

The innovative QIAGEN PCR Buffer facilitates the amplification of specific PCR products. During the annealing step of every PCR cycle, the buffer allows a high ratio of specific-tononspecific primer binding. Owing to a uniquely balanced combination of KCl and (NH4)2SO4, the PCR buffer provides stringent primer-annealing conditions over a wider range of annealing temperatures and Mg2+ concentrations than conventional PCR buffers.* Optimization of PCR by varying the annealing temperature or the Mg2+ concentration is dramatically reduced and often not required.


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