PCR stock solutions can also be decontaminated using UV light.
This method is laborious, however, and its efficiency is difficult
to control and cannot be guaranteed. We recommend storing solutions
in small aliquots and using fresh aliquots for each PCR.
Contamination by PCR product carry-over can be eliminated by
using the commercially available uracil-N-glycosylase (UNG). The procedure
involves substituting dUTP for dTTP in the PCR setup and treating
all PCR mixtures with UNG prior to PCR amplification. As a result,
any PCR product containing dUTP carried over from previous rounds
of amplification is destroyed by cleavage during the initial incubation
step that activates HotStarTaq DNA Polymerase.
Another approach to preventing amplification of contaminating
DNA is to treat individual reaction mixtures with DNase I or restriction
enzymes that cut between the binding sites of the amplification primers
used, before adding the template DNA sample.
* Most commercial bleach solutions are approximately 5.25% sodium
hypochlorate. Sodium hypochlorate is an irritant and should be handled
Product Warranty and Satisfaction Guarantee
QIAGEN guarantees the performance of all products in the manner described
in our product literature. The purchaser must determine the suitability
of the product for its particular use. Should any product fail to
perform satisfactorily due to any reason other than misuse, QIAGEN
will replace it free of charge or refund the purc
Source:Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 Related biology technology :1
. HotStarTaq DNA Polymerase2
. QIAGEN Multiplex PCR Handbook3
. TransMessenger Transfection Reagent Handbook4
. Transfection Reagent Selector Kit Handbook