can be used in subsequent experiments it is often necessary to remove
these contaminants. The QIAquick system offers a quick and easy
method for purifying the final PCR product. Using the MinElute
system, PCR products can be purified in higher concentrations due
to the low elution volumes needed in this system. For more information
about QIAquick and MinElute products, please call QIAGEN Technical
Services or your local distributor (see inside front cover).
8. Control of contamination
It is extremely important to include at least one negative control
that lacks the template nucleic acid in every PCR setup to detect
General physical precautions
Separate the working areas for setting up the PCR master mix
and DNA handling, including the addition of starting template, PCR
product analysis, or plasmid preparation. Ideally, use separate rooms.
Use a separate set of pipets for the PCR master mix. Use of
pipet tips with hydrophobic filters is strongly recommended.
Prepare and freeze small aliquots of primer solutions and dNTP
mix. Use of fresh distilled water is strongly recommended.
In case of contamination, laboratory benches, apparatus, and
pipets can be decontaminated by cleaning them with a 1/10 dilution
of a commercial bleach
solution.* Afterwards, the benches and pipets should be rinsed with
General chemical precautions
Source:Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 Related biology technology :1
. HotStarTaq DNA Polymerase2
. QIAGEN Multiplex PCR Handbook3
. TransMessenger Transfection Reagent Handbook4
. Transfection Reagent Selector Kit Handbook