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HotStarTaq PCR Handbook

roduct can be used in subsequent experiments it is often necessary to remove these contaminants. The QIAquick system offers a quick and easy method for purifying the final PCR product. Using the MinElute system, PCR products can be purified in higher concentrations due to the low elution volumes needed in this system. For more information about QIAquick and MinElute products, please call QIAGEN Technical Services or your local distributor (see inside front cover).

8. Control of contamination

It is extremely important to include at least one negative control that lacks the template nucleic acid in every PCR setup to detect possible contamination.

General physical precautions

Separate the working areas for setting up the PCR master mix and DNA handling, including the addition of starting template, PCR product analysis, or plasmid preparation. Ideally, use separate rooms.

Use a separate set of pipets for the PCR master mix. Use of pipet tips with hydrophobic filters is strongly recommended.

Prepare and freeze small aliquots of primer solutions and dNTP mix. Use of fresh distilled water is strongly recommended.

In case of contamination, laboratory benches, apparatus, and pipets can be decontaminated by cleaning them with a 1/10 dilution of a commercial bleach
solution.* Afterwards, the benches and pipets should be rinsed with distilled water.

General chemical precautions

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