Navigation Links
HotStarTaq PCR Handbook

e following steps 13 in protocols.
Add 25 l from the RT reaction to each PCR tube containing the master mix.
Continue with step 5 in the PCR protocols. Oligotex is not available in Japan.
* For further information about RNeasy, Oligotex, and Omniscript products, contact your local QIAGEN Technical Services or distributor (see inside front cover).
Please refer to the manufacturers instructions for the amount of enzyme required.

6. Touchdown PCR

Touchdown PCR uses a cycling program with varying annealing temperatures. It is a useful method to increase the specificity of PCR. The annealing temperature in the initial cycle should be 510C above the Tm of the primers. In subsequent cycles, the annealing temperature is decreased in steps of 12C/cycle until a temperature is reached that is equal to, or 25C below, the Tm of the primers. Touchdown PCR enhances the specificity of the initial primertemplate duplex formation and hence the specificity of the final PCR product.

To program your thermal cycler for touchdown PCR, you should refer to the manufacturers instructions.

7. Purification of PCR products

After amplification, the PCR sample contains a complex mixture of specific PCR product and residual reaction components such as primers, unincorporated nucleotides, enzyme(s), salts, mineral oil, and probably nonspecific amplification products. Before the specific PCR p
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36

Related biology technology :

1. HotStarTaq DNA Polymerase
2. QIAGEN Multiplex PCR Handbook
3. TransMessenger Transfection Reagent Handbook
4. Transfection Reagent Selector Kit Handbook
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: HotStarTaq PCR Handbook

(Date:7/31/2014)... even the entire body to visualize long-range connections between ... a long-time dream of biologists. A study published by ... Cell has now made that dream a ... and human tissue biopsies transparent, while keeping the cellular ... way for a better understanding of brain-body interactions, more ...
(Date:7/31/2014)... BLOOMINGTON, Ind. -- Chemists at Indiana University Bloomington ... in bricks-and-mortar fashion, a development with potential value ... as field-effect transistors and photovoltaic cells. , Their ... 3D Crystalline Solids and 2D Self-Assembled Crystals," has ... a journal of the Royal Society of Chemistry. ...
(Date:7/31/2014)... New York , July 31, 2014 ... by Transparency Market Research "Quantum Dots Market - Global ... - 2023," the market was valued at USD 88.5 ... 8,246.8 million by 2023, growing at a CAGR of ... full Quantum Dots Market Report at  http://www.transparencymarketresearch.com/quantum-dots.html ...
(Date:7/31/2014)... Graphite Metallizing Corporation announces GRAPHALLOY® bearings ... are uniquely suited to prevent loss of lubrication that ... high temperatures . , Recently, a manufacturer of ... at Graphite Metallizing to overcome problems with movement of ... pans containing blocks of powder mixture are loaded onto ...
Breaking Biology Technology:See-through organs and bodies will accelerate biomedical discoveries 2Chemists demonstrate 'bricks-and-mortar' assembly of new molecular structures 2Chemists demonstrate 'bricks-and-mortar' assembly of new molecular structures 3Global Quantum Dots Market is Expected to Reach USD 8,246.8 Million in 2023: Transparency Market Research 2Global Quantum Dots Market is Expected to Reach USD 8,246.8 Million in 2023: Transparency Market Research 3Global Quantum Dots Market is Expected to Reach USD 8,246.8 Million in 2023: Transparency Market Research 4Graphite Metallizing Announces Bearings for High Temperature Oven Applications 2Graphite Metallizing Announces Bearings for High Temperature Oven Applications 3
... SAN DIEGO, Oct. 27 BioAtom Inc. (private) announced,today ... Investor Conference on,Tuesday, October 28, 2008 at 2:30 PM ... in San Diego, California., Myron Karasik, M.B.A., C.M.C., ... of BioAtom Inc. of Costa Mesa, CA, dedicated,to development ...
... YORK, Oct. 24 The day of true ... study confirms the efficacy of a,revolutionary egg selection ... a viable and reliable fertility preservation option., ... ReproCure, a,vanguard genetics products company, this process increases ...
... /PRNewswire-FirstCall/ - AEterna Zentaris Inc.,(NASDAQ: AEZS ; ... therapy and oncology, today announced that it received ... Zentaris that during the 30,consecutive business days prior ... the,Company,s common shares had closed below the US$1.00 ...
Cached Biology Technology:BioAtom Inc. To Present at BIOCOM Investor Conference 2008 2Breakthrough Technology Takes Egg Freezing from Myth to Dependable Reality 2Breakthrough Technology Takes Egg Freezing from Myth to Dependable Reality 3Breakthrough Technology Takes Egg Freezing from Myth to Dependable Reality 4AEterna Zentaris Receives Notification from Nasdaq Relating to Minimum Bid Price 2AEterna Zentaris Receives Notification from Nasdaq Relating to Minimum Bid Price 3
(Date:7/31/2014)... An international team of scientists from Spain, France, ... rove beetle that is the oldest definitive member ... found in amber. The discovery and description were ... phase-contrast X-ray synchrotron imaging technique, which allows the ... amber. The new species is described in the ...
(Date:7/31/2014)... Edmond Becquerel first discovered the photovoltaic effect in 1839, ... power of sunlight for its own purposes. In a ... of the FASEB Journal , scientists ... power of sunlight by focusing on a naturally occurring ... evolution. This conservationor persistence over time and across speciessuggests ...
(Date:7/31/2014)... people are familiar with Hibiscus flowers- they are ... they are commonly planted in the landscape. Some, ... are endangered species. , Only a relatively few ... of an equally beautiful and intriguing related group ... "brother of Hibiscus". , Brother of Hibiscus species ...
Breaking Biology News(10 mins):Scientists shine bright new light on how living things capture energy from the sun 2Brother of Hibiscus is found alive and well on Maui 2
... researchers is examining a new vaccine method that may ... and his team of researchers is hoping to find ... smaller doses and require only one trip to the ... prepared from killed bacteria. The vaccinated person,s immune system ...
... of the future plan to confront the greatest scientific, ... inhabitants? This is the question being addressed at the ... Status and Perspective held by the Deutsche Forschungsgemeinschaft (DFG, ... Promotion of Science (JSPS), taking place in Hamburg this ...
... in French . A research ... Universitys Department of Microbiology and Immunology has discovered that ... the bodys autoimmune reactions may lose their effectiveness and ... type 1 diabetes. The study conducted on non-obese ...
Cached Biology News:Iowa State researchers look for smaller, cheaper, 1-dose vaccines 2Japanese and German researchers meet to discuss climate change 2Japanese and German researchers meet to discuss climate change 3Type 1 diabetes triggered by 'lazy' regulatory T-cells: McGill researchers 2