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HotStarTaq PCR Handbook

l reagents, and primers. Often, cycling parameters need to be changed. In many cases, results are still disappointing and further optimization is required. QIAGEN now offers the QIAGEN Multiplex PCR Kit, which eliminates the need for optimization, making the development of multiplex PCR assays both simple and fast. The QIAGEN Multiplex PCR Kit is the first kit specifically developed for multiplex PCR and provides an easy-to-use master-mix format. QIAGEN Multiplex PCR Master Mix contains pre-optimized concentrations of HotStarTaq DNA Polymerase and MgCl2, plus dNTPs and an innovative PCR buffer specially developed for multiplex PCR. The new PCR buffer contains a balanced combination of salts and additives, which enables comparable efficiencies for annealing and extension of all primers in the reaction. The kit is highly suited for multiplex PCR applications such as typing of genetically modified animals and plants, microsatellite analysis, determination of bacteria and viruses, or amplification of regions carrying SNPs. For more information about the QIAGEN Multiplex PCR Kit, contact your local QIAGEN Technical Services or distributor (see inside front cover).

5. RT-PCR
To perform PCR using RNA as a starting template, the RNA must first be reverse transcribed into cDNA in a reverse transcriptase reaction (RT reaction). Failure of the subsequent PCR is often a result of the limitations of the RT reaction. On average, only 1030% of the original RNA molecules is reverse transcribed into cDNA. The expression level of the target RNA molecules and the relati
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Related biology technology :

1. HotStarTaq DNA Polymerase
2. QIAGEN Multiplex PCR Handbook
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