( ...)HotStarTaq PCR Handbook

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10x PCR Buffer*
25 mM MgCl2
10 mM dNTP

Primer A
Primer B
5S ribosomal RNA (E. coli)
HotStarTaq DNA Polymerase
Distilled water
Single cell in 1x PCR Buffer 3 l
Variable
1 l

Variable
Variable
Variable
1 l
Variable
20 l 1x

200 M of
each dNTP
0.2 M
0.2 M
50 ng/reaction
5 units/reaction

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Total volume 50 l Multiplex PCR

Multiplex PCR is a demanding technique that requires extensive optimization of the amounts of Taq DNA Polymerase, MgCl2, additiona
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