Navigation Links
HotStarTaq PCR Handbook

orage of single cells: Single cells may be isolated by different methods (e.g., by flow cytometry or by micromanipulation).
Keep samples cool during the cell-isolation procedure to prevent DNA degradation.
Transfer cell into a PCR tube that has been filled with 20 l of 1x PCR Buffer. Immediately freeze the sample on dry ice.
Store cell at 80C until required for PCR analysis. PCR setup: Prepare a fresh master mix for single-cell PCR (see below).
Thaw the cells on ice.
Distribute 30 l of the master mix into each PCR tube, and place the tubes in the thermal cycler. Immediately start the cycling program with a 10 min incubation step at 95C to activate HotStarTaq DNA Polymerase for single-cell PCR. 50 cycles of PCR may be required to amplify a single-copy gene in one round of PCR. Master mix preparation: Prepare a master mix that has a final volume of 30 l per PCR, as detailed below.
Notes:
Addition of carrier nucleic acid is usually required (e.g., E. coli 5S rRNA).
Use polyacrylamide gel- or HPLC-purified primers only. Component Volume/reaction Final concentration
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36

Related biology technology :

1. HotStarTaq DNA Polymerase
2. QIAGEN Multiplex PCR Handbook
3. TransMessenger Transfection Reagent Handbook
4. Transfection Reagent Selector Kit Handbook
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: HotStarTaq PCR Handbook

(Date:11/26/2014)... PHILADELPHIA , Nov. 26, 2014 ... environments. Human exploration of space requires astronauts to maintain ... safety and success, and prevent potential errors and accidents. ... little is known about how cognition is affected by ... affected. Now, Penn Medicine researchers are poised ...
(Date:11/26/2014)... The ETC (Emerging Technology Centers) ... centers, announced today that applications for AccelerateBaltimore™ (AB) ... “Do you have a big idea?,” asked ETC’s ... know about it. AccelerateBaltimore helps you and your ... just 13 weeks.” Interested game changers with ...
(Date:11/26/2014)... 2014 SonaCare Medical, a leading ... devices, recently participated in the American Urology Association’s ... .” Key opinion leaders in urology presented on ... small renal masses while attendees had the opportunity ... in hands-on labs. Attendees at the Los Angeles, ...
(Date:11/26/2014)... (PRWEB) November 25, 2014 The Pittcon ... its official conference and exposition mobile app, ... free download in the App Store for iOS and ... valuable resource tool before, during and after the event. ... details on exhibiting companies, technical sessions, Conferee Networking sessions ...
Breaking Biology Technology:Penn Medicine Team Develops Cognitive Test Battery to Assess the Impact of Long Duration Spaceflights on Astronauts' Brain Function 2Penn Medicine Team Develops Cognitive Test Battery to Assess the Impact of Long Duration Spaceflights on Astronauts' Brain Function 3Penn Medicine Team Develops Cognitive Test Battery to Assess the Impact of Long Duration Spaceflights on Astronauts' Brain Function 4AccelerateBaltimore Applications to Close in 6 Days 2AccelerateBaltimore Applications to Close in 6 Days 3SonaCare Medical Supports American Urology Association’s Small Renal Mass 360° Summit 2SonaCare Medical Supports American Urology Association’s Small Renal Mass 360° Summit 3Pittcon Releases 2015 Mobile App 2
... MONTVALE, N.J., Dec. 12 Datascope Corp.,(Nasdaq: ... Co., one of the nation,s,leading independent proxy advisory ... -- James J. Loughlin and William,L. Asmundson -- ... December 20, 2007. To follow Glass Lewis, recommendation,Datascope ...
... Fourth Quarter Highlights:, -- Record revenues of $82.0 million, up 22% from the prior year,s fourth ... quarter ... $0.23, a 130% increase over last year,s ... of $24.3 million, revolving debt facility paid off, -- Launch of four new food and beverage products and ...
... Resource Group, LLC of,Birmingham, Alabama, the world,s ... with RJ Zapata & Associates S.A., one ... management companies in the,Dominican Republic, to develop ... in the country. The,two companies signed a ...
Cached Biology Technology:Glass Lewis Recommends Datascope Shareholders Vote for Re-Election of the Board's Directors 2Glass Lewis Recommends Datascope Shareholders Vote for Re-Election of the Board's Directors 3Glass Lewis Recommends Datascope Shareholders Vote for Re-Election of the Board's Directors 4Glass Lewis Recommends Datascope Shareholders Vote for Re-Election of the Board's Directors 5Glass Lewis Recommends Datascope Shareholders Vote for Re-Election of the Board's Directors 6Glass Lewis Recommends Datascope Shareholders Vote for Re-Election of the Board's Directors 7Martek Announces Fourth Quarter and FY 2007 Financial Results 2Martek Announces Fourth Quarter and FY 2007 Financial Results 3Martek Announces Fourth Quarter and FY 2007 Financial Results 4Martek Announces Fourth Quarter and FY 2007 Financial Results 5Martek Announces Fourth Quarter and FY 2007 Financial Results 6Martek Announces Fourth Quarter and FY 2007 Financial Results 7Martek Announces Fourth Quarter and FY 2007 Financial Results 8Martek Announces Fourth Quarter and FY 2007 Financial Results 9Martek Announces Fourth Quarter and FY 2007 Financial Results 10Martek Announces Fourth Quarter and FY 2007 Financial Results 11Martek Announces Fourth Quarter and FY 2007 Financial Results 12Martek Announces Fourth Quarter and FY 2007 Financial Results 13Martek Announces Fourth Quarter and FY 2007 Financial Results 14Martek Announces Fourth Quarter and FY 2007 Financial Results 15Masada Teams With Leading Waste Management Firm to Produce Ethanol From Municipal Solid Waste in the Dominican Republic 2
(Date:11/6/2014)... -- A groundbreaking paper from a team of Florida ... of how plants could adapt to and survive environmental ... published in the latest issue of the journal ... (the complex of DNA and proteins) is organized in ... that some genes are turned on and others are ...
(Date:11/5/2014)... RENO – Kelly Redmond has dedicated his career ... knowledge to a general audience. , As deputy ... Climate Center at Nevada,s Desert Research Institute, Redmond ... the management, application and dissemination of climate data ... Annual Fall Meeting in San Francisco on December ...
(Date:11/4/2014)... — Think about the way our bodies are assembled ... know that they are supposed to become a nerve ... find the correct place and alignment? Researchers at the ... , In a new study, UM researchers describe the ... surrounding neighbors, at the head-trunk region. Their discovery may ...
Breaking Biology News(10 mins):Maize analysis yields whole new world of genetic science 2Nevada climatologist to be honored at AGU Fall Meeting for lifetime of public outreach 2The inside story: How the brain and skull stay together 2
... Forschungsgemeinschaft (DFG, German Research Foundation) is intensifying ... its autumn meeting in Bonn the Grants ... the establishment of 13 additional Research Training ... Training Groups. These enable doctoral researchers to ...
... Diego, November 18, 2008 The American Journal of ... 2008), will be featured at the "Changing Climate ... the event is Guest Editor Howard Frumkin, MD, DrPH, ... health. "Climate change is a key public health ...
... 2008) − A new study confirms that exercise can ... stem cells in the hippocampus of the mouse brain, ... brain chemical which promotes the production and maturation of ... cells differentiate into a variety of mature nerve cells ...
Cached Biology News:National and international doctoral training 2National and international doctoral training 3National and international doctoral training 4National and international doctoral training 5National and international doctoral training 6National and international doctoral training 7Issues at intersection of climate change and health impact global well-being 2Issues at intersection of climate change and health impact global well-being 3Issues at intersection of climate change and health impact global well-being 4Exercise increases brain growth factor and receptors, prevents stem cell drop in middle age 2Exercise increases brain growth factor and receptors, prevents stem cell drop in middle age 3
Anti-Rsk2/MAPKAP Kinase 1b, #0 SK2/MAPKAP K1 beta Smartpool&t 3T3 cell lysate Non-specific Control Pool , #D 1X Universal Buffer...
50 assays. Provides an easy system for rapid separation of cytosol and particulate fractions that are compatiable with downstream studies....
... Transformation Buffer Set are convenient methods for ... for simple and highly efficient DNA transformation. ... for heat shocking and related procedures. Instead, ... adding DNA to Z-Competent cells and incubating ...
... The Light Diagnostics Universal ... intended for use in the ... DNA generated by in ... research use only; not to ...
Biology Products: