Navigation Links
HotStarTaq PCR Handbook

1. Too much starting template Check the concentration and storage conditions of the starting template (see appendix). Make serial dilutions of template nucleic acid from stock solutions. Perform PCR using these serial dilutions. When re-amplifying a PCR product, start the re-amplification round using 1 l of a 1-in- 103104 dilution of the previous PCR. In most cases, a nested PCR approach results in higher specificity and sensitivity for reamplification (see appendix). 2. Carry-over contamination If the negative-control PCR (without template DNA) shows a PCR product or a smear, exchange all reagents. Use disposable pipet tips containing hydrophobic filters to minimize cross-contamination. Set up all reaction mixtures in an area separate from that used for DNA preparation or PCR product analysis. 3. Enzyme concentration too high When using HotStarTaq DNA Polymerase, use 2.5 units per 100 l reaction. When using HotStarTaq Master Mix, use 25 l Master Mix per 50 l reaction. 4. Too many cycles Reduce the number of cycles in steps of 3 cycles. 5. Mg2+ concentration not optimal Perform PCR with different final concentrations of
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36

Related biology technology :

1. HotStarTaq DNA Polymerase
2. QIAGEN Multiplex PCR Handbook
3. TransMessenger Transfection Reagent Handbook
4. Transfection Reagent Selector Kit Handbook
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: HotStarTaq PCR Handbook

(Date:7/24/2014)... July 24, 2014 -- Keryx Biopharmaceuticals, Inc. (Nasdaq:KERX) (the ... randomized, active control Phase 3 study of Zerenex (ferric ... for the treatment of hyperphosphatemia in patients with end-stage ... binding and iRon delivery with FErric CiTrate in EsrD) ... the American Society of Nephrology ( JASN ). ...
(Date:7/24/2014)... 2014  Asterias Biotherapeutics, Inc. (OTCBB: ASTYV), a ... regenerative medicine, announced today that it will host ... on Tuesday, July 29, 2014, at 4:30 p.m. ... an overview of Asterias, business strategy and product ... http://www.ustream.tv/channel/asterias-biotherapeutics at least 15 minutes before the ...
(Date:7/24/2014)... computers have yet to materialise. Yet, scientists are ... such computers faster. One such approach relies on ... by applying an electric field. A new study ... coupled quantum dots (TQDs) with electrical impulses can ... example, should TQDs be used as quantum information ...
(Date:7/24/2014)... , July 24, 2014 Research and ... "Global Genomics and Proteomics Analytical Instruments Market 2014-2018" ... http://photos.prnewswire.com/prnh/20130307/600769 Genomics is the study of ... study of the structure and functions of proteomes or ... tools and technology. Genomics involves the mapping of genes ...
Breaking Biology Technology:Zerenex (ferric citrate) long-term Phase 3 study results published in JASN 2Zerenex (ferric citrate) long-term Phase 3 study results published in JASN 3Zerenex (ferric citrate) long-term Phase 3 study results published in JASN 4Asterias Biotherapeutics Announces Live Investor Webcast 2Asterias Biotherapeutics Announces Live Investor Webcast 3Asterias Biotherapeutics Announces Live Investor Webcast 4Global Genomics and Proteomics Analytical Instruments Market 2014-2018: Key Vendors are Agilent, Bio-Rad , F. Hoffmann, La Roche, Illumina, PerkinElmer and Thermo Fisher Scientific 2
... is writing a series of articles on raising capital from investors. ... Most of the time when raising equity capital, the offering terms ... sheet . When dealing with angel investors , it ... , ,Sometimes prospective angel investors will want to negotiate the initial ...
... Oren Harari is coming to Milwaukee next week for ... , ,Not just to manufacturers, who he will be addressing at ... organizations in a number of sectors, including technology. , ,Based on ... in a Copycat Economy , he intends to deploy some provocative ...
... - Mithridion, Inc ., a biopharmaceutical start up that ... has closed an additional $1.2 million of Series A funding ... round of funding brings the total equity capital raised in ... initial funding target. Mithridion will use the funds to develop ...
Cached Biology Technology:Capital-raising term sheets for angels and venture capitalists 2Capital-raising term sheets for angels and venture capitalists 3Capital-raising term sheets for angels and venture capitalists 4Capital-raising term sheets for angels and venture capitalists 5Visions: Innovating like Madonna and Willie Nelson (and Steve Jobs) 2Visions: Innovating like Madonna and Willie Nelson (and Steve Jobs) 3Alzheimer's drug developer lands $1.2M in venture funding 2Alzheimer's drug developer lands $1.2M in venture funding 3
(Date:7/27/2014)... of "big data," a single computer cannot always find ... be distributed across a cluster of computers that analyze ... Google mine your web history to present you with ... next favorite book or movie. But big data is ... for monitoring brain activity are generating unprecedented quantities of ...
(Date:7/25/2014)... on the land now called California, and many of ... plants they used for food, fuel, tools, and ritual. ... habitat and natural resources. , Frank Lake, an ecologist ... lead a field trip to the Stone ... America,s 99th Annual Meeting, in Sacramento, Cal. this August. ...
(Date:7/25/2014)... (July 24, 2014)The link between autism and disrupted ... puzzle of the disease, and is largely unknown. ... Autism Research Initiative (SFARI), George Washington University (GW) ... offer truly integrative and in-depth answers to these ... , LaMantia, professor of pharmacology and physiology ...
Breaking Biology News(10 mins):New tools help neuroscientists analyze 'big data' 2New tools help neuroscientists analyze 'big data' 3New tools help neuroscientists analyze 'big data' 4New tools help neuroscientists analyze 'big data' 5Fire ecology manipulation by California native cultures 2Fire ecology manipulation by California native cultures 3GW researcher receives grant to answer the how and why of autism during development 2
... July 9, 2012 Bovine spongiform encephalopathy (BSE, or "mad ... portions of the brain to turn sponge-like. This transmissible ... of protein known as a prion, rather than by a ... signs of illness is about 60 months. Little is known ...
... miles at 150mph, how long will it take to reach ... question makes you apprehensive, then you may have mathematics anxiety. ... journal Behavioral and Brain Functions reports that a ... both genders, performance is likely to be affected as a ...
... MAResearchers from Brigham and Women,s Hospital (BWH) have made a ... The team, led by Thomas S. Kupper, MD, chair of ... that high expression of a cell-signaling molecule, known as interleukin-9, ... findings will be published online in the July 8, 2012 ...
Cached Biology News:Study finds 'mad cow disease' in cattle can spread widely in ANS before detectable in CNS 2Girls' mathematics performance more likely to suffer than boys' as a result of mathematics anxiety 2Researchers discover molecule in immune system that could help treat dangerous skin cancer 2
384 Well PCR Plate Carrier for Thermo-Sealer...
... Collection of Cell Cultures (ECACC) has been ... academia and government institutes for over seven ... designed to introduce candidates to cell culture ... on more specialist areas such as planning ...
...
... used to perform runs with and ... Experion automated electrophoresis system. The software ... gel views and summarizes size, concentration, ... table. This software is for use ...
Biology Products: