ution enables amplification of a reaction which
: Q-Solution increases PCR specificity in certain primertemplate
: Q-Solution has no effect on PCR performance.
: Q-Solution causes reduced efficiency or failure of a previously
successful amplification reaction. In this case, addition of Q-Solution
disturbs the previously optimal primertemplate annealing. Therefore,
when using Q-Solution for the first time for a particular primertemplate
system, always perform reactions in parallel with and without Q-Solution.
HotStarTaq DNA Polymerase requires an activation step of 15
min at 95C (see step 6 of this protocol).
When using Q-Solution for the first time in a particular primertemplate
system, it is important to perform parallel amplification reactions
with and without Q-Solution.
Set up all reaction mixtures in an area separate from that
used for DNA preparation or PCR product analysis.
Use disposable tips containing hydrophobic filters to minimize
If required, prepare a dNTP mix containing 10 mM of each dNTP.
Store this mix in aliquots at 20C.
1. Thaw 10x PCR Buffer, dNTP mix, primer solutions, and Q-Solution.
It is important to mix the solutions completely before use to avoid
localized concentrations of salts. When using Q-Solution, additional
MgCl2 is not usually
Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 Related biology technology :1
. HotStarTaq DNA Polymerase2
. QIAGEN Multiplex PCR Handbook3
. TransMessenger Transfection Reagent Handbook4
. Transfection Reagent Selector Kit Handbook