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HotMasterAn innovative Hot Start/Cold Stop technology for better ,,, PCR* results




Conclusions

Eppendorf HotMaster Taq DNA Polymerase offers several advantages over other Hot Start DNA polymerases. First and foremost, HotMaster Taq both reduces non-specific PCR products and increases specific yield to a greater extent than the major Hot Start DNA polymerases on the market. This is primarily due to the significant advantages of this kit such as Hot Start/Cold Stop technology. In addition, no heat activation is required with HotMaster, and target sizes of up to 5.0 kb can be efficiently and reproducibly amplified. Furthermore, the Mg++ concentration is self-adjusting, which makes it optimal for virtually all targets. Lastly, because of its unique technology, HotMaster will not contaminate PCR reactions with unwanted proteins as do the Hot Start DNA polymerases that employ antibodies to achieve inhibition. Testing has also shown that HotMaster Taq can be used in real time PCR applications (data not shown).


*This product is sold under licensing arrangements with F. Hoffmann-La Roche Ltd., Roche Molecular Systems, Inc., and Applied Biosystems.








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