Navigation Links
Homogeneous, chemiluminescent kinase assay kits for assessing the functional activity of a broad range of serine-threonine and tyrosine kinases

J. Turner
GE Healthcare, The Maynard Centre, Cardiff, UK


Competitive binding assays have been developed for measuring the functional activity of a range of serine-threonine and tyrosine kinases. Based on enzyme fragment complementation and the subsequent hydrolysis of chemiluminescent substrate, HitHunter™ Kinase Assay Kits generate a signal that can be read either on high-throughput imaging platforms or on standard microplate readers.


Introduction
Human protein kinases are involved in numerous and diverse cellular processes such as signal transduction and cell cycle control. The precise control of protein phosphorylation is fundamental to normal cellular behaviour and uncontrolled signaling by kinases has been implicated in disease states such as cancer, athersclerosis, psoriasis, and in inflammatory responses such as septic shock. Inhibitors that block the activity of protein kinases and phosphatases are therefore potentially useful for the development of therapeutic agents.

HitHunter Kinase Assay Kits are competitive binding assays utilizing enzyme fragment complementation (EFC) for the sensitive and simple measurement of a range of biologically important targets. Based on proprietary kinase antibody/substrate pairs, they provide a sensitive and homogeneous method for measuring the activity of a broad range of serine-threonine and tyrosine kinases covering 80% of the kinome.


HitHunter Kinase Assay principle
EFC technology is based on the recombination of two inactive β-galactosidase (β-gal) fragments; complementation of the enzyme donor (ED) fragment and the enzyme acceptor (EA) fragment forms the active tetrameric β-gal (Fig 1). Subsequent hydrolysis of luminescent substrate generates a signal that can be easily read on standard microplate reader
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. LiquiChip Kits for bead-based cytokine and kinase assays
2. Setting up a kinase profiler with IMAP
3. TKXtra DataFile: Homogenous tyrosine kinase detection using High Efficiency Fluorescence Polarization
4. Enzyme Immunoassay for Studying Intracellular Levels of cAMP
5. Quality control of antibodies using the 2100 bioanalyzer and the Protein 200 Plus assay
6. High-content screening of GPCR activation with MetaXpress, AcuityXpress and the Transfluor assay system
7. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
8. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
9. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
10. Is Z factor the best assessment for the quality of cellular assays delivering higher content?
11. The measurement of DNA condensation and mitochondrial changes during apoptosis and necrosis in cell based assays
Post Your Comments:
(Date:12/17/2014)... , Dec. 17, 2014 Tigercat ... Phase 2 study evaluating the investigational oral NK-1 ... patients with severe, chronic itch who are unresponsive ... care, including topical steroids and antihistamines. ... in Visual Analog Scale (VAS) itch score from ...
(Date:12/15/2014)... & PETACH TIKVAH, Israel ... (NASDAQ: BCLI ), a leading developer of ... that wholly-owned subsidiary Brainstorm Cell Therapeutics Ltd. was awarded ... Israel,s Office of the Chief Scientist (OCS).  ... in receipt of grant support from the Office of ...
(Date:12/15/2014)... Dec. 15, 2014  Origin Agritech Limited (NASDAQ GS: SEED) ... supplier of crop seeds in China , ... fiscal year 2014 ended September 30, 2014, before the market ... will host a teleconference on January 8, 2015, at 8:00 ... time to discuss the results. To participate in the call, ...
(Date:12/13/2014)... - MaRS and Virgin Unite Canada, the non-profit foundation of ... to support Canadian entrepreneurs tackling social and environmental challenges. Today, ... the MaRS Centre to announce the new partnership, which includes ... fund has $1 million in seed capital provided by Virgin ... Social Innovation Foundation, founded by Alison Lawton . ...
Breaking Biology Technology:Velocity Pharmaceutical Development, LLC and Tigercat Pharma, Inc. Announce Phase 2 Results for VPD-737 in Patients With Chronic Pruritus 2Velocity Pharmaceutical Development, LLC and Tigercat Pharma, Inc. Announce Phase 2 Results for VPD-737 in Patients With Chronic Pruritus 3Velocity Pharmaceutical Development, LLC and Tigercat Pharma, Inc. Announce Phase 2 Results for VPD-737 in Patients With Chronic Pruritus 4BrainStorm Receives $1,100,000 for its 8th Annual Non-Dilutive Grant from Israel's Chief Scientist Office 2BrainStorm Receives $1,100,000 for its 8th Annual Non-Dilutive Grant from Israel's Chief Scientist Office 3Origin Agritech Limited to Announce Fiscal 2014 Annual Financial Results on January 8th, 2015 2Origin Agritech Limited to Announce Fiscal 2014 Annual Financial Results on January 8th, 2015 3MaRS and Virgin Unite partner to invest in business as a force for good 2MaRS and Virgin Unite partner to invest in business as a force for good 3
... million, -- Quarterly Net Income Increases 76.5% to ... YORK and HAIKOU, Hainan, China, May 2 /Xinhua-PRNewswire-FirstCall/,-- ... Board: CPHI),which develops, manufactures, and markets generic and ... results,for the first quarter of 2008., ...
... May 2 eResearchTechnology, Inc.,(eRT), (Nasdaq: ... (ECG) collection and interpretation services,announced today that ... and,Chief Executive Officer, and Richard Baron, the ... at the Deutsche Bank 33rd Annual,Healthcare Conference ...
... 2 /PRNewswire-FirstCall/ - GeneNews Limited (TSX: GEN), ... for the early,detection of diseases and personalized ... for the first quarter ended March 31,2008., ... of the key milestones,necessary to support the ...
Cached Biology Technology:China Pharma Holdings, Inc. Announces First Quarter 2008 Financial Results 2China Pharma Holdings, Inc. Announces First Quarter 2008 Financial Results 3China Pharma Holdings, Inc. Announces First Quarter 2008 Financial Results 4China Pharma Holdings, Inc. Announces First Quarter 2008 Financial Results 5China Pharma Holdings, Inc. Announces First Quarter 2008 Financial Results 6China Pharma Holdings, Inc. Announces First Quarter 2008 Financial Results 7China Pharma Holdings, Inc. Announces First Quarter 2008 Financial Results 8China Pharma Holdings, Inc. Announces First Quarter 2008 Financial Results 9eResearchTechnology to Present at the Deutsche Bank 33rd Annual Healthcare Conference on May 7th 2008 2GeneNews Announces First Quarter Results 2GeneNews Announces First Quarter Results 3GeneNews Announces First Quarter Results 4GeneNews Announces First Quarter Results 5GeneNews Announces First Quarter Results 6GeneNews Announces First Quarter Results 7GeneNews Announces First Quarter Results 8
(Date:12/10/2014)... -- CIE San Diego has launched an innovative trust ... that enhances care coordination among social service and care ... earned a second $1 million grant from the Alliance ... in community and; will be recognized as a "Live ... 4-6p. CIE San Diego today announced the ...
(Date:12/5/2014)... Utah , Dec. 4, 2014  Tute Genomics, ... raised $2.3 million in Series A1 funding led by ... investors also participated in the investment round. ... the healthcare community adopts next-generation sequencing and seeks new ... said Reid Robison , MD MBA, and CEO ...
(Date:11/21/2014)... According to a new market research report ... Control, Intrusion Detection, Parking Management, Under Vehicle Inspection), End ... - Global Forecasts to 2020", published by MarketsandMarkets, The ... $25 Billion in 2014 and is expected to reach ... 8.69%. Browse 116 market data Tables and ...
Breaking Biology News(10 mins):Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 2Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 3Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 4Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 2Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 3Industrial Security Systems Market Worth $38 Billion by 2020 2Industrial Security Systems Market Worth $38 Billion by 2020 3
... June 09, 2009 / b3c newswire / - ... Netherlands Organisation for Applied Scientific Research TNO today announced ... further develop Amarna’s viral gene delivery platform SVac ... for the manufacture, formulation and testing of viral gene ...
... axial complex of sea urchins ( Echinoidea ), an ... that group of marine invertebrates there exists a structural ... in BioMed Central,s open access journal Frontiers in ... structural data available on a given organ in combination ...
... Merck Irving S. Sigal Memorial Award is being presented ... Infectious Disease Initiative at the Broad Institute of MIT ... Medicine at Harvard Medical School. Sponsored by Merck Research ... presented in memory of Irving S. Sigal, who was ...
Cached Biology News:Amarna Therapeutics B.V. and TNO announce SVac research and development partnership 2Amarna Therapeutics B.V. and TNO announce SVac research and development partnership 3
Proteinase K Buffer 3.9 ml...
Rabbit polyclonal to VEGF Receptor 2 (phospho Y1214) ( Abpromise for all tested applications). entrezGeneID: 3791 SwissProtID: P35968...
Glutathione Agarose Beads 10 ml...
Anti-Ephrin B1 Host: rabbit polyclonal Species Reactivity: human Applications: WB Storage: 4C...
Biology Products: