Navigation Links
Homogeneous, chemiluminescent kinase assay kits for assessing the functional activity of a broad range of serine-threonine and tyrosine kinases

J. Turner
GE Healthcare, The Maynard Centre, Cardiff, UK


Competitive binding assays have been developed for measuring the functional activity of a range of serine-threonine and tyrosine kinases. Based on enzyme fragment complementation and the subsequent hydrolysis of chemiluminescent substrate, HitHunter™ Kinase Assay Kits generate a signal that can be read either on high-throughput imaging platforms or on standard microplate readers.


Introduction
Human protein kinases are involved in numerous and diverse cellular processes such as signal transduction and cell cycle control. The precise control of protein phosphorylation is fundamental to normal cellular behaviour and uncontrolled signaling by kinases has been implicated in disease states such as cancer, athersclerosis, psoriasis, and in inflammatory responses such as septic shock. Inhibitors that block the activity of protein kinases and phosphatases are therefore potentially useful for the development of therapeutic agents.

HitHunter Kinase Assay Kits are competitive binding assays utilizing enzyme fragment complementation (EFC) for the sensitive and simple measurement of a range of biologically important targets. Based on proprietary kinase antibody/substrate pairs, they provide a sensitive and homogeneous method for measuring the activity of a broad range of serine-threonine and tyrosine kinases covering 80% of the kinome.


HitHunter Kinase Assay principle
EFC technology is based on the recombination of two inactive β-galactosidase (β-gal) fragments; complementation of the enzyme donor (ED) fragment and the enzyme acceptor (EA) fragment forms the active tetrameric β-gal (Fig 1). Subsequent hydrolysis of luminescent substrate generates a signal that can be easily read on standard microplate reader
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. LiquiChip Kits for bead-based cytokine and kinase assays
2. Setting up a kinase profiler with IMAP
3. TKXtra DataFile: Homogenous tyrosine kinase detection using High Efficiency Fluorescence Polarization
4. Enzyme Immunoassay for Studying Intracellular Levels of cAMP
5. Quality control of antibodies using the 2100 bioanalyzer and the Protein 200 Plus assay
6. High-content screening of GPCR activation with MetaXpress, AcuityXpress and the Transfluor assay system
7. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
8. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
9. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
10. Is Z factor the best assessment for the quality of cellular assays delivering higher content?
11. The measurement of DNA condensation and mitochondrial changes during apoptosis and necrosis in cell based assays
Post Your Comments:
(Date:10/22/2014)... Shimadzu Scientific Instruments introduces its Open Solution QuantAnalytics(TM) ... management and clinical markets. The software increases the speed ... users to highlight and review results that are of ... to filter results by group or based on a ... need to sift through analytes of no interest. In ...
(Date:10/22/2014)... 22, 2014 Physicians Choice Laboratory ... pharmacogenetics menu, which enables healthcare providers to individualize ... PCLS’s evidence-based results, healthcare providers are better able ... therapy, while minimizing risks for adverse drug reactions ... to the FDA [1] more than 2.2 million ...
(Date:10/22/2014)... Research and Markets  has announced the addition of ... Geography - Global Analysis and Forecast (2014 - 2020)" ... electronics, also called as polymer electronics or plastic electronics, ... small conductive molecules and electrically conductive polymers. It is ... carbon based, made using synthetic strategies developed in the ...
(Date:10/20/2014)... PARK, Calif. , Oct. 20, 2014 /PRNewswire/ ... today that the Company has signed a Notice ... of Regenerative Medicine (CIRM), effective October 1, 2014.  ... Clinical Development payments and the release of additional ... million CIRM grant award for clinical development of ...
Breaking Biology Technology:Shimadzu Releases QuantAnalytics Open Access LCMS Software Package for Clinical Applications 2PCLS Announces Expansion of Cardiovascular Pharmacogenetic Testing Menu 2PCLS Announces Expansion of Cardiovascular Pharmacogenetic Testing Menu 3Global Organic Electronics (Semiconductor, Conductive, Dielectric and Substrate) Market - Analysis and Forecast (2014 - 2020) 2Global Organic Electronics (Semiconductor, Conductive, Dielectric and Substrate) Market - Analysis and Forecast (2014 - 2020) 3Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 2Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 3Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 4
... SOUTH SAN FRANCISCO, Calif., May 15 Poniard Pharmaceuticals, ... focused on oncology, today announced that it has filed ... Securities and Exchange Commission (SEC) that, when declared effective ... time to offer and sell up to $60 million ...
... JOLLA, Calif., May 15 Fate Therapeutics, ... exclusive intellectual property rights jointly owned by Children,s Hospital ... covers compositions and methods for supporting hematopoietic stem cells. ... director of the Stem Cell Program at Children,s Hospital ...
... Shandong, China, May 15 /PRNewswire-Asia-FirstCall/ -- China,Biologic Products, ... the,Company,), one of the leading plasma-based pharmaceutical companies ... that the Company,s,management will present at the upcoming ... New York, NY on May 18, 2009 and ...
Cached Biology Technology:Poniard Pharmaceuticals Files Shelf Registration 2Poniard Pharmaceuticals Files Shelf Registration 3Fate Therapeutics Granted Exclusive License for Stem Cell Modulators by Children's Hospital Boston and Massachusetts General Hospital 2Fate Therapeutics Granted Exclusive License for Stem Cell Modulators by Children's Hospital Boston and Massachusetts General Hospital 3China Biologic Products to Present at CCG IR's China Rising Conference and Oppenheimer's China Dragon Call Conference in New York 2China Biologic Products to Present at CCG IR's China Rising Conference and Oppenheimer's China Dragon Call Conference in New York 3China Biologic Products to Present at CCG IR's China Rising Conference and Oppenheimer's China Dragon Call Conference in New York 4
(Date:10/17/2014)... critical and pertinent for practicing physicians and clinicians given ... The Journal, Disaster Medicine and Public Health Preparedness ... Public Health, to surround the public, medical professionals and ... , On October 17, the journal published ... was prepared by Dr. Eric Toner, internist and emergency ...
(Date:10/17/2014)... from the University of Copenhagen have shown for the ... lungs of Cystic fibrosis patients, giving them the opportunity ... in chronic infections. , The study also discovered the ... (CF) patients was halted or slowed down by the ... all the oxygen and helped "suffocate" the bacteria, forcing ...
(Date:10/16/2014)... the human body. Battles are won, lost or ... this stalemate—known as tumor dormancy—can last up to ... that is poorly understood. , A new ... Torquato, a Professor of Chemistry at Princeton University, ... and the switch to a malignant state. Published ...
Breaking Biology News(10 mins):Scientists opens black box on bacterial growth in cystic fibrosis lung infection 2Modeling tumor dormancy 2
... cardiovascular disorders and old age have one thing in ... scientists have observed oxidative changes in important biomolecules. These ... free radicals that are formed as a by-product of ... acids. Today, reactive oxygen molecules are no longer ...
... CAMBRIDGE, Mass.--As Charles Darwin showed nearly 150 years ago, bird ... team of MIT mathematicians and engineers has now explained exactly ... gravity and transport food into their mouths. The phalarope, ... interactions between its beak and water droplets to propel bits ...
... Contrary to long-held assumptions, high-salt diets may ... investigators from the Albert Einstein College of Medicine ... examining dietary intake among a nationally representative sample ... actually observed a significantly increased risk of death ...
Cached Biology News:Biosensor for measuring stress in cells 2MIT solves gravity-defying bird beak mystery 2MIT solves gravity-defying bird beak mystery 3New study casts further doubt on risk of death from higher salt intake 2
DTT, 25g...
... Agarose overlays are commonly used ... commercial "DNA grade" agaroses either ... to insect cells or lack ... obtain good overlays. Novagen's BacPlaque ...
Antibody specific for trimethyl-Lys27 of histone H3. Mouse monoclonal. Antigen: Synthetic peptide derived from histone H3, trimethylated at lysine 27...
...
Biology Products: