Navigation Links
Highly Sensitive microRNA Array Performance

ay in the same bladder, lung, and uterus total RNA samples that were used for array analysis. The mature miRNAs on the Northern blots were quantified by phosphorimaging. The hybridization assays used chemiluminescent reactions and detection. The mirVana miRNA array system accurately reflected the expression level of each miRNA as compared to the hybridization assay and Northern analysis data obtained from total RNA (Figure 4).

Figure 4. miRNA Array Quantitation. The fold change in expression of seven different miRNAs within three different pairs of tissues was determined using three independent methods of quantitation--Northern analysis, solution hybridization/digestion assay (Hyb/Dig'n), and microarray analysis. The high correlation between the three methods validates the miRNA expression data obtained from the array system.


Biological Validation

A final validation of the procedure as well as a confirmation of the biological significance of miRNAs in adult human tissues involved profiling miRNA expression in biological samples. We isolated miRNA from 26 normal human tissues. Half of each sample was used to create an miRNA pool containing miRNA from all 26 samples. The miRNAs from the pooled sample as well as each of the 26 single-tissue samples were fluorescently labeled with Cy3 or Cy5 using the mirVana miRNA Labeling Kit. Each single tissue miRNA sample was hybridized to the miRNA arrays along with a pooled sample to act as a common reference. The relative signal intensities of the single and pooled samples were compared for each miRNA. Each of the 26 normal
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Highly Active and Removable AffinityTagged Lambda Phosphatase
2. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
3. The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids
4. Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
7. Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
8. Sensitive detection of tumor cells in peripheral blood of carcinoma patients by a reverse transcription PCR method
9. Novel MicroRNA Array Technology for Sensitive miRNA Profiling
10. Sensitive identification of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
11. Simultaneous Detection of CYP3A4, CYP2D6 and CYP2C9 Metabolites with a Single, Sensitive, LC/MS/MS Method
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Highly Sensitive microRNA Array Performance

(Date:12/22/2014)... , Dec. 22, 2014 /PRNewswire/ - RepliCel Life Sciences ... medicine company focused on the development of autologous cell ... of the University of Calgary in conjunction with co-authors ... which further validates the company,s ongoing clinical research using ... of pattern baldness. The paper entitled " ...
(Date:12/22/2014)... 2014  ( www.competitivehealth.com ) — Competitive Health is ... review and advocacy service, has signed an agreement to ... discount health services marketplace. 63% of Americans ... they expected to pay. As part of an ongoing ... Savings is pleased to offer medical bill review and ...
(Date:12/22/2014)... Fertility Associates of Memphis has announced ... , a network of more than 20 contributing ... have fast access to a national pool of frozen ... Fertility Associates of Memphis is the only fertility clinic ... , Dr. William Kutteh , director of Fertility ...
(Date:12/19/2014)... BioPlus Specialty Pharmacy (BioPlus), one ... of Nick Maroulis, Pharm.D. to the newly created position ... In this position, Dr. Maroulis will continue to manage ... of our multi-site pharmacies as the company expands. Dr. ... that time he has served in many different capacities ...
Breaking Biology Technology:Newly Released Peer-Reviewed Publication Further Validates RepliCel's Use of Dermal Sheath Cup Cells to Treat Pattern Baldness 2Newly Released Peer-Reviewed Publication Further Validates RepliCel's Use of Dermal Sheath Cup Cells to Treat Pattern Baldness 3Newly Released Peer-Reviewed Publication Further Validates RepliCel's Use of Dermal Sheath Cup Cells to Treat Pattern Baldness 4Newly Released Peer-Reviewed Publication Further Validates RepliCel's Use of Dermal Sheath Cup Cells to Treat Pattern Baldness 5WellCard Savings Announces CoPatient Medical Bill Review Service for Members 2Fertility Associates of Memphis Announces Partnership with Donor Egg Bank USA 2Fertility Associates of Memphis Announces Partnership with Donor Egg Bank USA 3BioPlus Specialty Pharmacy Promotes Dr. Nick Maroulis to Vice President of Specialty Pharmacy Services 2
... ARAY ), a global leader in the field of ... Euan S.,Thomson, Ph.D., is scheduled to present at the ... Thursday, September 25, 2008 at 9:00 a.m.,EDT (6:00 a.m. ... be available online from the,investor relations page of the ...
... teamed with a decades-old drug to beat atherosclerotic plaques ... in St. Louis. , The scientists have found that ... of tiny blood vessels that feed arterial plaques. Their ... vessels from restarting their growth, which could shrink or ...
... Business Expected -, HOUSTON, Sept. 15 ... an initial assessment, its corporate,headquarters in Houston, TX ... However, the facility could remain without power ... operating out of its disaster recovery,facility., Cyberonics ...
Cached Biology Technology:Accuray Incorporated's CEO to Speak at UBS Global Life Sciences Conference 2Old and new therapies combine to tackle atherosclerosis 2Old and new therapies combine to tackle atherosclerosis 3Cyberonics Provides Update on Business Operations Following Hurricane Ike 2Cyberonics Provides Update on Business Operations Following Hurricane Ike 3
(Date:12/10/2014)... December 9, 2014   ... Platform   Jifflenow, ... scheduling solutions for business-to-business (B2B) events, today announced ... in mobile near-field communication (NFC), Bluetooth low energy ... (Logo: http://photos.prnewswire.com/prnh/20140612/691055) , Jifflenow will integrate ...
(Date:12/3/2014)... 2, 2014 As part of our commitment ... is pleased to announce the release of a new ... collect the workforce data that they need. ... left by existing readers. Many such devices have serious ... modern technology. Older models force users to navigate numerous ...
(Date:11/21/2014)... 20, 2014   Atmel® Corporation (NASDAQ: ... and touch technology solutions, today launched the industry,s first ... the widest V cc range from 1.7V to ... faster I 2 C bus communication speeds, and are ... making them ideal for consumer, industrial, computer, and medical ...
Breaking Biology News(10 mins):Jifflenow And ITN International Bring Cutting-Edge Badge Scanning Technology To B2B Events 2Jifflenow And ITN International Bring Cutting-Edge Badge Scanning Technology To B2B Events 3Inception Technologies to Release New Biometric Reader 2Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 2Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 3Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 4
... Yurttas Beim, PhD, Founder and CEO of the ... in the genetics of female infertility, presented A Report ... Revolution at the Futures in Reproduction Conference at the ... spirit of inquiry personified by Nobel Prize winner Professor ...
... 14, 2012 A fast growing, flesh-eating fungus killed ... Mo., according to two new studies based on genomic ... the U.S. Centers for Disease Control and Prevention (CDC). ... by the fungus Apophysomyces , according to the ...
... At crucial points in the metabolism of all organisms, ... Factor P (EF-P, for short) takes center stage. What ... been elucidated by researchers at Ludwig-Maximilians-Universitt in Munich. ... of Microbiology at LMU, actually focused on how bacteria ...
Cached Biology News:Report from the front lines of personalized reproductive medicine revolution 2Report from the front lines of personalized reproductive medicine revolution 3Fungus responsible for 5 deaths in the wake of massive tornado 2Fungus responsible for 5 deaths in the wake of massive tornado 3Raising the blockade 2
Human Presenilin-2 NTF Affinity Purified Polyclonal Ab...
... Cryo/CryoPlus Inventory Racks for ... available with cardboard or stainless ... or 5ml cryo vials. ... racks available. Platform risers ...
... This volume provides excellent background information, detailed ... of major HIV proteins including: the HIV ... and protease. Expression of HIV proteins using ... assays; HIV/CD4 interactions; transient assays for Nef-induced ...
Monkey IL-2 ELISPOT Kit with white/opague microtiter plates...
Biology Products: