Navigation Links
Highly Sensitive microRNA Array Performance

the indicated amounts of synthetic miR-124 spiked into the sample following fractionation. The signal is determined by background subtraction, and is depicted in signal/noise units. The heat map on the graph shows the Log2(R/G Normalized Ratio) (Mean) of miR-124 from the same samples.


High Target Specificity

A number of miRNAs have very similar sequences. For example, there are eight variants of the let-7 family that differ by one to four nucleotides. Part of the development of the mirVana miRNA array system involved creating probes and hybridization and wash conditions that minimized cross-hybridization between closely related miRNAs. While we were unable to distinguish single nucleotide mismatches without significantly reducing sensitivity, we were able to identify conditions that provide specificity and sensitivity. To test specificity, our model system included seven different arrays with probes targeting 40 different miRNAs. Each of the seven arrays included a different miR-16 probe--one was completely homologous to miR-16, two had distinct single mismatches, two had double mismatches, and two had three mismatches. miRNA from human tissue was isolated, labeled, and hybridized to each array. Signal from the miR-16 element was normalized using the signal from the other forty elements on each array. The hybridization and wash conditions used with the miRNA arrays provided over 10-fold more signal for perfect matches than for miRNAs with <90% target homology (Figure 2). Identical experiments with miR-23 probes yielded similar results.

Figure 2. miRNA Array Specificity. Probe specifi
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Highly Active and Removable AffinityTagged Lambda Phosphatase
2. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
3. The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids
4. Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
7. Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
8. Sensitive detection of tumor cells in peripheral blood of carcinoma patients by a reverse transcription PCR method
9. Novel MicroRNA Array Technology for Sensitive miRNA Profiling
10. Sensitive identification of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
11. Simultaneous Detection of CYP3A4, CYP2D6 and CYP2C9 Metabolites with a Single, Sensitive, LC/MS/MS Method
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Highly Sensitive microRNA Array Performance

(Date:9/18/2014)... YARDLEY , Pennsylvanie, 18 septembre 2014 ... Lyon en 1974, a été ... à compléter les évaluations cliniques traditionnelles par ... Reported Outcomes) et sur les impacts économiques ... http://photos.prnewswire.com/prnh/20140618/119365 A cours ...
(Date:9/18/2014)... Now a team led by an engineer at Michigan ... that will let scientists slash the cost of one ... Syringe pumps are used to dispatch precise amounts of ... a reaction. They can also cost hundreds or even ... of Michigan Tech students published the library of free ...
(Date:9/18/2014)... (PRWEB) September 18, 2014 Silicon-valley Electronic ... with Digitaliza TXT , the latest addition to ... its Clinical Research Organization (CRO) Program, Clinovo further expands ... three days of ClinCapture training, we are now able ... CEO Ana Pulido. “We can also rely on Clinovo ...
(Date:9/17/2014)... 2014 A long-term retrospective study of ... (APBI) via interstitial brachytherapy suggests that women younger ... could be suitable to receive the treatment. Current ... these patients in the "unsuitable" category for APBI. ... meeting, Beaumont Health System,s Peter Y. Chen ...
Breaking Biology Technology:1974 - 2014 - Mapi fête ses 40 ans 2Doing science just got cheaper -- and faster 2Digitaliza TXT Joins Clinovo’s CRO Partnership Program, Bringing Its Open Source EDC System, ClinCapture to Latin America 2Digitaliza TXT Joins Clinovo’s CRO Partnership Program, Bringing Its Open Source EDC System, ClinCapture to Latin America 3Partial Breast Irradiation With Interstitial Brachytherapy Appears Appropriate For Women Previously Considered Unsuitable 2Partial Breast Irradiation With Interstitial Brachytherapy Appears Appropriate For Women Previously Considered Unsuitable 3Partial Breast Irradiation With Interstitial Brachytherapy Appears Appropriate For Women Previously Considered Unsuitable 4
... my wishes are,coming true," says Kristin Shumpert, who is traveling from ... of Las,Vegas ... and to meet the people who "saved my ... held little interest to the young,woman who suffers from a devastating ... time to use the ladies, room. , ...
... Integrated Knowledge Center Outside the United States. , ... ... Knowledge Center (DKC) today, 17 months after breaking ground last summer. ... the city’s Genome Valley, the center will be a research hub ...
... commercial life sciences sales experience to Cyntellect , ... a privately-held life sciences company leading the development and commercialization ... it has appointed Andre Lubarsky as the Company,s Director of ... sales of Cyntellect,s cell purification and cell health products in ...
Cached Biology Technology:Noninvasive Medical Technologies Helps Make Young Tennessee Woman's Wishes Come True 2Noninvasive Medical Technologies Helps Make Young Tennessee Woman's Wishes Come True 3Noninvasive Medical Technologies Helps Make Young Tennessee Woman's Wishes Come True 4Noninvasive Medical Technologies Helps Make Young Tennessee Woman's Wishes Come True 5DuPont Opens Research & Development Center in India 2DuPont Opens Research & Development Center in India 3Cyntellect Appoints Former BD Biosciences Executive Andre Lubarsky as Director of Sales - North America 2
(Date:9/18/2014)... AZ, Sept. 18, 2014 -- Arizona turned its newborn ... just a few short months, earning it the first-ever Newborn ... The March of Dimes presented Will Humble, M.P.H., Arizona,s Department ... held here today. Mr. Humble established a policy of full ... to send newborn blood samples to the lab for analysis, ...
(Date:9/18/2014)... will be celebrated tonight at the third annual Golden ... health of premature infants and in paving the way ... work was supported by the National Science Foundation, the ... Commission, will be honored at a ceremony at the ... of Members of Congress will be on hand to ...
(Date:9/18/2014)... September 18, 2014 Elsevier, a world-leading provider ... services, and the Australasian Research Management Society (ARMS), ... scientists at the 5th Scopus Young Researcher of ... earlier today at the National Convention Centre and ... was attended by guests representing Australia,s and New ...
Breaking Biology News(10 mins):March of Dimes honors Arizona with first-ever newborn screening award 2March of Dimes honors Arizona with first-ever newborn screening award 33rd annual Golden Goose award ceremony honors 8 researchers; Unusual work had big results 2Elsevier and Australasian Research Management Society award scientists at Scopus ceremony 2
... fluctuations in the populations they target, but its not ... journal Nature by scientists at Scripps Institution of Oceanography ... general underlying mechanism. Research led at Scripps with ... two chief scientific advisors to the United Kingdom) demonstrates ...
... worm, University of Maryland researchers have discovered an important ... absorbed and transported into the body. The finding could ... the worlds number one nutritional disorder., With C. elegans, ... Hamza, assistant professor of animal and avian sciences, and ...
... -- Some primates have evolved big brains because their ... advantage that outweighs the demands of extra years of ... Duke University and the University of Zurich have concluded ... key benchmarks in the development of 28 different primate ...
Cached Biology News:Fishing throws targeted species off balance, Scripps study shows 2Fishing throws targeted species off balance, Scripps study shows 3Bloodless worm sheds light on human blood, iron deficiency 2Bloodless worm sheds light on human blood, iron deficiency 3Slowly-developing primates definitely not dim-witted 2Slowly-developing primates definitely not dim-witted 3
Mouse monoclonal antibody to Lats2....
... (eIF4E-binding protein II) is involved in translation ... kidney. PHAS proteins bind to eIF-4E, the ... capped mRNA in vitro and in cells. ... of PHAS/eIF-4E complexes. This allows eIF-4E to ...
MAb to HSV-2, gG-2 HSV-2 Glycoprotein G-2...
... INK4a-ARF locus encodes two unrelated proteins both ... arrests the cell cycle in a p53-dependent ... the rapid degradation of mdm2 proteinrequired for ... accumulation. This interaction is mediated by ...
Biology Products: