Navigation Links
Highly Sensitive microRNA Array Performance

the indicated amounts of synthetic miR-124 spiked into the sample following fractionation. The signal is determined by background subtraction, and is depicted in signal/noise units. The heat map on the graph shows the Log2(R/G Normalized Ratio) (Mean) of miR-124 from the same samples.


High Target Specificity

A number of miRNAs have very similar sequences. For example, there are eight variants of the let-7 family that differ by one to four nucleotides. Part of the development of the mirVana miRNA array system involved creating probes and hybridization and wash conditions that minimized cross-hybridization between closely related miRNAs. While we were unable to distinguish single nucleotide mismatches without significantly reducing sensitivity, we were able to identify conditions that provide specificity and sensitivity. To test specificity, our model system included seven different arrays with probes targeting 40 different miRNAs. Each of the seven arrays included a different miR-16 probe--one was completely homologous to miR-16, two had distinct single mismatches, two had double mismatches, and two had three mismatches. miRNA from human tissue was isolated, labeled, and hybridized to each array. Signal from the miR-16 element was normalized using the signal from the other forty elements on each array. The hybridization and wash conditions used with the miRNA arrays provided over 10-fold more signal for perfect matches than for miRNAs with <90% target homology (Figure 2). Identical experiments with miR-23 probes yielded similar results.

Figure 2. miRNA Array Specificity. Probe specifi
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Highly Active and Removable AffinityTagged Lambda Phosphatase
2. Gene Expression Arrays: Highly Sensitive Detection of Expression Patterns with Improved Tools for Target Amplification
3. The DIG System Nonradioactive and Highly Sensitive Detection of Nucleic Acids
4. Detection of Variation in Highly Polymorphic Mhc Genes by Denaturing Gradient Gel Electrophoresis Using the DCode System
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
7. Simple, Sensitive Isotyping of Mouse Monoclonal Antibodies
8. Sensitive detection of tumor cells in peripheral blood of carcinoma patients by a reverse transcription PCR method
9. Novel MicroRNA Array Technology for Sensitive miRNA Profiling
10. Sensitive identification of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
11. Simultaneous Detection of CYP3A4, CYP2D6 and CYP2C9 Metabolites with a Single, Sensitive, LC/MS/MS Method
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Highly Sensitive microRNA Array Performance

(Date:11/18/2014)... Philadelphia, PA (PRWEB) November 18, 2014 ... healthcare planning and design expertise to the nation's hospitals ... is best served by those dedicating their careers to ... As a respected contributor in her respective field, Laura ... engineering expertise at the 2014 Winter Simulation Conference. ...
(Date:11/18/2014)... Pa. , Nov. 18, 2014  Novira Therapeutics, ... for curative treatment of chronic hepatitis B virus (HBV) ... M.D., was appointed to its Scientific Advisory Board (SAB).  ... join our SAB," said Christian S. Schade , ... of clinical development experience which will be invaluable to ...
(Date:11/16/2014)... 2014 James Hill and Ken Crooks ... Hoods, 3 Years Later” scheduled for Thursday, November 20, ... Butler University surprised many in the lab design domain ... chemistry and general chemistry teaching laboratories at Gallahue Hall. ... age and substantial growth within the chemistry program. Now, ...
(Date:11/15/2014)... The International Society for ... the society’s Governing Board has grown with the ... scientific officer and Dr. Charles Simonton, divisional vice ... Mr. Rowe and Dr. Simonton are both ... , Dr. Nabil Dib, ISCTR President stated, ...
Breaking Biology Technology:Array Healthcare Simulation Expert Slated to Speak at Winter Simulation Conference 2Novira Therapeutics Announces Appointment of Brian F. Daniels, M.D., to its Scientific Advisory Board 2Novira Therapeutics Announces Appointment of Brian F. Daniels, M.D., to its Scientific Advisory Board 3Don't miss the I2SL High-Tech Talks Webinar Series Presenting: Butler University's Renovation With Filtered Fume Hoods, 3 Years Later 2Don't miss the I2SL High-Tech Talks Webinar Series Presenting: Butler University's Renovation With Filtered Fume Hoods, 3 Years Later 3International Society for Cardiovascular Translational Research Governing Board Adds Medical Industry Leaders 2
... particles have been used to bring stem cells to ... to increase the capacity of cells to repair damaged ... disciplinary research, published in The Journal of the ... technique where endothelial progenitor cells a type of ...
... same methods used in fusion-energy research to create extremely ... required to make future computer chips. Current technology ... computer chips in a process called photolithography, which involves ... material, then chemically etching the resulting pattern. New ...
... , , , ... , , ALAMEDA, Calif., Aug. 18 Today, ... becoming the premier producer of low-cost advanced biofuels. Through ... base algae strains to more than double CO2 consumption and fuel ...
Cached Biology Technology:Nanomagnets guide stem cells to damaged tissue 2Nuclear fusion research key to advancing computer chips 2Nuclear fusion research key to advancing computer chips 3Aurora Biofuels Breakthrough Doubles CO2 Uptake and Fuel Production 2
(Date:11/6/2014)... occur because of metastasis, yet progress in preventing ... , "It,s been particularly challenging to design drugs ... fellow in systems biology at Harvard Medical School. ... after they,ve already metastasized." , Gujral and colleagues ... help researchers better understand how metastasis begins. Their ...
(Date:11/5/2014)... -Individuals show great diversity in their ability to identify ... greatly differ in their perceptual evaluation of odors, with ... , Sex differences in olfactory detection may ... be connected to one,s perception of smell, which is ... olfactory superiority has been suggested to be cognitive or ...
(Date:11/4/2014)... examining only marketing directed at children on the ... found that the majority of black, middle-income and ... tactics. , Authored by Arizona State University researcher ... the first to examine the use of child-directed ... food restaurants and its relationship to demographics. It ...
Breaking Biology News(10 mins):Migration negation 2Migration negation 3The female nose always knows: Do women have more olfactory neurons? 2Fast food marketing for children disproportionately affects certain communities 2Fast food marketing for children disproportionately affects certain communities 3
... enormous carbon footprint, but often energy is being wasted ... Research to be published in the International Journal ... the ubiquity of smart phones connected to the office ... heating or air conditioning for unused spaces. Bruce ...
... YORK, NY (December 5, 2013) For discoveries about how ... part of the foundation of memoryColumbia University will award the ... and May-Britt Moser, PhD, of the Norwegian University of Science ... University College London in the UK. Their work, conducted in ...
... growth in the Arctic is boosted by global warming, it,s ... of the permafrost beneath the surface, University of Florida researchers ... to simulate thawing of permafrost in a warming world. ... exists in the atmosphere today, and what happens to it ...
Cached Biology News:The heat is on...or off 2Columbia's 2013 Horwitz Prize awarded for discoveries that could lead to new Alzheimer's treatments 2Columbia's 2013 Horwitz Prize awarded for discoveries that could lead to new Alzheimer's treatments 3Columbia's 2013 Horwitz Prize awarded for discoveries that could lead to new Alzheimer's treatments 4Columbia's 2013 Horwitz Prize awarded for discoveries that could lead to new Alzheimer's treatments 5UF researchers' experiment is first to simulate warming of Arctic permafrost 2UF researchers' experiment is first to simulate warming of Arctic permafrost 3
Goat polyclonal to NIR1 ( Abpromise for all tested applications). Antigen: Synthetic peptide: SWARGPPKFESVP, corresponding to C terminal amino acids 962-974 of NIR1. Entrez Gene ID: 83394 ...
PMM2 Immunogen: PMM2 (NP_000294, 47 a.a. ~ 112 a.a) partial recombinant protein with GST tag. Storage: -20 C, Avoid Freeze/Thaw Cycles *Available for distribution in the US only...
HOXC11 Antibody...
PPOX Immunogen: PPOX (AAH02357, 378 a.a. ~ 478 a.a) partial recombinant protein with GST tag. Storage: -20 C, Avoid Freeze/Thaw Cycles *Available for distribution in the US only...
Biology Products: