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Highly Sensitive microRNA Array Performance

mirVana miRNA Probe Set and mirVana miRNA Labeling Kit
Detecting miRNAs on Microarrays

MicroRNAs (miRNAs) are extremely small RNA species that represent approximately 0.01% of the total mass of RNA in a sample (10 g total RNA contains ~1 ng miRNA). Due to their small size and low abundance, miRNAs can be extremely difficult to detect using standard array procedures. To address such limitations, Ambion scientists have developed the mirVana miRNA array system, consisting of:

Highly specific miRNA Probes (the mirVana miRNA Probe Set). The mirVana miRNA Probe Set includes amine-modified oligonucleotides with sequences complementary to the known human, mouse, and rat miRNAs. The probes have a polynucleotide spacer that ensures maximal hybridization efficiency to labeled miRNAs when the probes are arrayed on amine-reactive slides.

Robust miRNA Sample Labeling (the mirVana miRNA Labeling Kit). The mirVana miRNA Labeling Kit features a tailing procedure that adds 2050 nucleotides to the 3' ends of all miRNAs in a sample. This strategy maximizes signal from each miRNA without jeopardizing hybridization due to labeling. The 3' tail that is added is a mixture of standard and amine-modified nucleotides.
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