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Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell,,,Line

kground measurement.

Fig.1

Conclusions

Purification of plasmid DNA with the StrataPrep kit is simple to perform and generates high yields of pure DNA. Because endotoxin is effectively removed, high-efficiency transfection of endotoxin-sensitive mammalian cells is easily achieved.

REFERENCES

  1. Basehore, S. and Braman, J. (1999) Strategies 12: 5-7.

  2. Cotton, M., et al. (1994) Gene Therapy 1: 239-246.

  3. Weber, M., et al. (1995) BioTechniques 19: 930-939.

  4. Sweet, M. and Hume, D. (1996) J. Leukocyte Biol. 60: 8-26.

  5. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, Second Edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.

  6. Held, P. (1998) Pharmaceutical Lab. February: 4-6.


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