Navigation Links
High-throughput gene expression analysis to screen for anti-asthma drugs

Andreas Pahl, Uwe Oelmller,* and Susanne Ullmann*
Department of Pharmacology, University of Erlangen, Erlangen, Germany

* QIAGEN GmbH, Hilden Germany Drug library screening based on gene expression analysis requires reliable and reproducible methods of RNA purification and sensitive, quantitative, real-time RT-PCR analysis. This article demonstrates how RNeasy 96 technology, automated on the BioRobot Gene Expression workstation, enabled reproducible, high-throughput sample preparation. One-step, real-time RT-PCR using the QuantiTect Probe RT-PCR Kit provided highly sensitive gene expression analysis to identify potential drugs to fight asthma.

Bronchial asthma is one of the commonest chronic diseases in modern society and the most common chronic childhood disease. The pathogenesis and causes of asthma are still poorly understood. Nonetheless, our knowledge of asthma pathogenesis has increased greatly over the past two decades, partially through recognition of the critical role played by cytokines in the inflammatory response.

Many cytokines are involved in asthma. Although some of these cytokines are involved in many inflammatory diseases, others are more specific to allergic inflammation, such as asthma. These cytokines include interleukins (IL) IL-4 and IL-13, which are derived from T-helper 2 (Th2) cells. Th1 cells, which are involved in cellular immunity, secrete other cytokines, such as IL-2. Gene expression analysis of these cytokines provides valuable information about the effects of potential drugs for asthma.

The goal of our research is to identify potential new drugs that selectively
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Quantitative end-point RT-PCR gene expression measurement using the Agilent 2100 Bioanalyzer and standardized RT-PCR
2. Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
3. Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Varian Cary Eclipse
4. Successful stabilization of gene expression profiles
5. Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci
6. Flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer and on-chip staining
7. 2100 expert software Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer
8. Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis
9. Microfluidic analysis of multiplex PCR products for the genotyping of Helicobacter pylori
10. High resolution DNA analysis with the DNA 500 and DNA 1000 LabChip kits
11. Extended data analysis using DeCyder EDA
Post Your Comments:
(Date:7/30/2015)... 2015 AACC welcomed thousands of medical ... Annual Meeting & Clinical Lab Expo in ... meeting showcased revolutionary advancements in clinical testing technology ... providers to diagnose patients quickly and accurately and ... As of Wednesday, July 29, more ...
(Date:7/30/2015)... , July 30, 2015 ... by Product (Software, Molecular Modeling, PB/PK, PKPD, Trial Design, ... & Development, Preclinical, Clinical Trial), by End Users - ... Market is expected to reach USD 2,107.99 million by ... CAGR of 15.29%. Browse more than ...
(Date:7/30/2015)... -- With over 60,000 customers across the globe, ISN improves the efficiency and effectiveness of ... than 60,000 safe and reliable contractors in over 80 countries. ... ... Over 60,000 Worldwide Customers In More ... Headquartered in Dallas, TX , ISN has additional offices ...
(Date:7/30/2015)... ... 2015 , ... As part of its 2015 growth plan and as a follow up to ... business units, Whitehouse Laboratories is pleased to announce that it has begun construction on a ... Excellence and will be strictly dedicated to basic USP 51, USP 61, and USP 62 ...
Breaking Biology Technology:Innovative Research on Cancer, Heart Disease, and Infectious Diseases Draws More than 17,500 Attendees to 2015 AACC Annual Meeting 2Innovative Research on Cancer, Heart Disease, and Infectious Diseases Draws More than 17,500 Attendees to 2015 AACC Annual Meeting 3Innovative Research on Cancer, Heart Disease, and Infectious Diseases Draws More than 17,500 Attendees to 2015 AACC Annual Meeting 4Biosimulation Market Worth 2,107.99 Million USD by 2020 2Biosimulation Market Worth 2,107.99 Million USD by 2020 3Biosimulation Market Worth 2,107.99 Million USD by 2020 4ISN Reaches Over 60,000 Worldwide Customers 2ISN Reaches Over 60,000 Worldwide Customers 3Whitehouse Labs Formally Announces Plans to Open a Microbiological Laboratory 2
... Feb. 7, 2011 Tengion, Inc. (Nasdaq: TNGN ... Nichtberger, M.D., President and Chief Executive Officer of Tengion, will ... Conference on Monday, February 14, 2011, at 2:00 p.m. EST. ... "IPO Window-Creaking Open or Wide Open?" the following day on ...
... Cmed Technology , ... a roundtable and give a presentation about electronic data ... for Clinical Ops Executives ( SCOPE ) conference at ... http://photos.prnewswire.com/prnh/20100707/NY31446LOGO) As part of SCOPE,s two-day "Electronic ...
... Berkeley, have found a way to grow nanolasers directly onto ... new class of faster, more efficient microprocessors, as well as ... They describe their work in a paper to be ... journal Nature Photonics . "Our results impact ...
Cached Biology Technology:Tengion to Present at the 13th Annual BIO CEO & Investor Conference and the Leerink Swann 'Hot Topics in Therapeutics' Roundtable Conference 2Cmed Technology to Host Roundtable, Give Presentation About EDC Pain Points at Summit for Clinical Ops Executives (SCOPE) 2Cmed Technology to Host Roundtable, Give Presentation About EDC Pain Points at Summit for Clinical Ops Executives (SCOPE) 3Engineers grow nanolasers on silicon, pave way for on-chip photonics 2Engineers grow nanolasers on silicon, pave way for on-chip photonics 3
(Date:7/27/2015)... ANGELES , July 27, 2015   ... evidence- and experience-based clinical improvement solutions, today announced ... solution is now available on Android smartphones and ... inpatient and post-care organizations can use ZynxCarebook to ... same patient,s condition, streamline care transitions to other ...
(Date:7/21/2015)... -- Today, ZTE announced its Android smartphone Axon, featuring ... revenues in 2015 that relate to sales of FPC1025 for this ... 2,200 MSEK for 2015. Jörgen Lantto, CEO of FPC, ... China and we are proud that ... Axon , its first ...
(Date:7/20/2015)... , July 20, 2015  Acuity Market ... Report: The Convergence of Commerce and Privacy" forecasts ... mobile biometric apps will be downloaded to smart ... The mobile biometrics market is projected to generate ... revenue during the seven-year forecast period.    ...
Breaking Biology News(10 mins):Zynx Health Adds Android Device Support to ZynxCarebook Solution 2Zynx Health Adds Android Device Support to ZynxCarebook Solution 3FPC's Touch Fingerprint Sensor FPC1025 in ZTE's Smartphone Axon 2Biometrics on Smart Mobile Devices to Redefine Digital Identity with 12.9 Billion Biometric App Downloads between 2014 and 2020 2
... an unlikely new target: molecules. Scientists at the National ... of molecular data similar to Facebook,s recently debuted graph ... to find all their New York-living, beer-drinking buddies in ... rapidly sift through enormous chemical and biological data sets ...
... July 18, 2013 -- Scientists at Wake Forest Baptist Medical ... effect of impacts to the head incurred by football players. ... capture players, exposure to the risk of concussion over the ... magnitude of all impacts, said senior author of the study ...
... Health Summit held at the Mayflower Renaissance hotel in Washington ... to scientists, writers and researchers looking to learn and discuss ... links mushrooms to today,s and future health issues. ... world,s top scientists, researchers and nutrition experts who will provide ...
Cached Biology News:Facebook for molecules 2Facebook for molecules 3Scientists develop new way to measure cumulative effect of head hits in football 2Scientists develop new way to measure cumulative effect of head hits in football 3
... Kit, 500 reactions. Prepare circular DNA ... h.Generate microgram quantities of template DNA ... amplified DNA directly for cycle sequencing ... M13 liquid cultures, colonies, plaques, or ...
...
Request Info...
Spaced cover glasses to promote effective hybridization...
Biology Products: