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High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS

and poor MS detection sensitivity of the small and polar molecules of the drug residues. The sample preparation is simple and fast with good recoveries. To meet the regulatory needs, two fragment ions per analyte are used for more confident confirmation. There are two identical product ion pairs for AH and AOZ chosen for MRM quantitation because they give the best sensitivity. With the TM LC/MS/MS System LINAC collision cell feature, a fast analysis can be achieved without chromatographic separation of the two compounds with the same product ions. There was no cross-talk when they are co-eluted from the LC. The validated method has high sensitivity with the LODs between 0.15-0.05 ng/g chicken tissue and excellent linearity and reproducibility. The method is robust and has been used for routine analysis of nitrofuran antibiotics in animal tissue.

References

1 A. Leitner, P. Zollner, W. Lindner, J. Chromatogr. A, 2001, 939, 49-58.

Acknowledgements

We would like to acknowledge Tum Sutthisak of GeneSystems, Thailand, Prasert Kaewchuchuon, Michael Koenig of Applied Biosystems, SE Asia for their technical and management support.


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