Navigation Links
High resolution DNA analysis with the,,,DNA 500 and DNA 1000 LabChip kits

a standard deviation of 10 % or less. The DNA 500 assay relies, like the other DNA assays, on an intercalating dye for fluorescence detection. The dye incorporation depends on the basepair composition and the secondary structure of the DNA fragments. For larger fragments such sequence differences are usually statistically evened out. For shorter fragments, however, the sequence can make a difference in dye incorporation. Therefore, deviations from the sizing and quantitative specifications can occur for individual fragments below 70 bp. The same samples shown in the chip-based electropherograms in figure 1 were compared to a slab gel separation using a commercially available 4- 20 % gradient gel (Novex, USA).

As shown in figure 2, the Agilent 2100 bioanalyzer performs with equal or better resolution than the slab gel, delivering a clearer image of the sample.

DNA 1000 LabChip kit
In the size range below 500 bp, the DNA 1000 LabChip kit shows a performance that is comparable to the DNA 500 LabChip kit. The operating range was extended to 1000 bp, to better match the size range, in which most PCR fragments fall. From 500 to 1000 bp the resolution of the DNA 1000 assay is 10 %, while the maximum error for quantitation remains at 30 % (this corresponds to a 95 % confidence level).
The DNA 1000 kit was used to optimize PCR conditions for a competitive RT-PCR reaction of two genes: GAPDH and the heat shock protein hsp721 . The goal of the experiment was to achieve a co-amplification of GAPDH (housekeeping gene) and hsp72. The hsp72 was the gene of interest, the expression of which can be influenced by various factors. For the experiment, RNA was isolated from HepG2-cells and reverse transcribed into cDNA. The cDNA was amp
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Analysis of urine and seawater samples by ultrasonic nebulization with a high resolution ICP spectrometer
2. High-throughput gene expression analysis to screen for anti-asthma drugs
3. Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci
4. Flow cytometric analysis of human primary cells using the Agilent 2100 bioanalyzer and on-chip staining
5. 2100 expert software Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer
6. Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis
7. Microfluidic analysis of multiplex PCR products for the genotyping of Helicobacter pylori
8. Extended data analysis using DeCyder EDA
9. 2D-LC analysis of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
10. Neurite outgrowth cell-by-cell analysis using the IN Cell Developer Toolbox
11. Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: High resolution DNA analysis with the DNA and DNA LabChip kits

(Date:12/24/2014)... , Dec. 24, 2014 PlasmaTech Biopharmaceuticals, Inc. (NASDAQ: ... areas, announced the closing of an underwritten public offering of ... to an aggregate 3,500,000 shares of common stock, at an ... The warrants have a per share exercise price of $5.00, ...
(Date:12/24/2014)... BioLife Solutions , Inc. (NASDAQ: BLFS ), a ... hypothermic storage and cryopreservation freeze media and ... ("BioLife" or the "Company"), today announced that it will hold ... (the "Annual Meeting"). Because the expected date for ...
(Date:12/24/2014)... 23, 2014 GMO corn cases filed across ... in the process of being consolidated in a Kansas federal ... Syngenta AG MIR 162 Corn Litigation, MDL No. 2591 in ... Management of the Syngenta GMO corn multidistrict litigation (MDL) has ...
(Date:12/24/2014)... MA (PRWEB) December 23, 2014 ... is designed to introduce cutting edge communication technology, ... of platform upgrades. This webinar series demonstrates online ... quest to leverage web conferencing’s most innovative and ...
Breaking Biology Technology:PlasmaTech Biopharmaceuticals, Inc. Announces Closing of Public Offering 2PlasmaTech Biopharmaceuticals, Inc. Announces Closing of Public Offering 3BioLife Solutions Sets Date for Annual Meeting of Stockholders 2Carey Danis & Lowe Reports on Syngenta GMO Corn Transfer Order 2SoundConnect Launches Leading Edge Business Communications Webinar Series 2SoundConnect Launches Leading Edge Business Communications Webinar Series 3
... , , , 77.6.6 Sample cooling ... If sensitive samples should remain cooled until the PCR takes place, the , ... ways: , , , ... Adapt an initial program and connect via a "LINK" command. , ...
... , , , 7.6.4 Long PCR , ... programmed as desired with every temperature , command, enables a , ... , With Long PCR, this function can be ... elongation. , , ...
... , , , There is obviously ... to all PCR amplifications. For example, , depending on the ... of the PCRs can differ from each other. Finding the optimal or the , ... and efficient amplification of a specific target. , ...
Cached Biology Technology:7 Programming 2PCR Optimization 2PCR Optimization 3
(Date:1/22/2015)... 2015 NXT-ID, Inc. (NASDAQ: NXTD and NXTDW) a ... the recent success of the Wocket™ smart wallet at CES 2015 in ... was named as one of the "11 Hot Products at CES" in ... At CES So Far" by Newseveryday.com and "The top 10 gadgets from ...
(Date:1/22/2015)... YORK , Jan. 21, 2015  Analyst Report Issued by ... Card Brands report showed that planet-wide, transactions at merchants on ... while the total number of credit, debit, and prepaid cards ... U.S. were victims of fraudulent card usage in 2012, and ...
(Date:1/22/2015)... Research and Markets ( http://www.researchandmarkets.com/research/wxdglm/global_wearable ) ... Wearable Technologies Market and Applications, Opportunities, Industry Analysis, ... 2014-2020" report to their offering. , ... can be worn on the user,s body and ...
Breaking Biology News(10 mins):CES Response for NXT-ID's Wocket Smart Wallet Kicks Off 2015 2CES Response for NXT-ID's Wocket Smart Wallet Kicks Off 2015 3$4.80 Near Term Price Target with "Buy" given by CapRock Research for NXT-ID, Inc. (NASDAQ: NXTD) issued by Small Cap IR 2Global Wearable Technologies Market and Applications, Opportunities, Industry Analysis, Segmentation, Size, Growth, Share, Overview, Trends and Forecast 2014-2020 2
... two human beings are the same. Although we all ... many places. And since genes provide the blueprint for ... in protein function. But what impact does this diversity ... Universitaet Muenchen (TUM) have investigated how protein function is ...
... and wastewater managers are missing substantial opportunities to save ... (Sept. 4) by Water in the West, a research ... Nexus: A Literature Review," also identifies the amount of ... oil and coal, and to generate electricity. , The ...
... Researchers have known for some time that the blood vessels ... the body are more than just bodily pipelines. Arterioles and ... regulating the flow of the blood they,re carrying. Biomedical engineers ... mathematical model that explains just how they do it. ...
Cached Biology News:Wide range of differences, mostly unseen, among humans 2Report reveals missed opportunities to save water and energy 2Drexel researchers looking inside vessels to understand blood's ebb and flow 2
With L-glutamine and potassium bicarbonate. Sterile, Insect cell culture tested...
Mol wt: average mol wt12,360.96 Da by calculation...
Originally developed for the culture of Drosophila; suitable for culture of other dipteran cell lines. Powder, Insect cell culture tested...
... Adaptor encodes a T7 promoter, lac ... translation initation codon and an N-terminal GST•Tag ... reported to enhance the production and in ... partners. The GST•Tag is a multi-purpose tag ...
Biology Products: