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High resolution DNA analysis with the,,,DNA 500 and DNA 1000 LabChip kits

Slab gel analysis is the traditional technique used for DNA analysis. By comparing the separated bands of an unknown sample to a known standard (size or mass ladder), the size and quantitation of each fragment can be estimated. The Agilent 2100 bioanalyzer is an instrument, which utilizes LabChip technology to achieve DNA and RNA analysis on micro-fabricated chips. This approach entails significant advantages including increased speed of analysis, improved data precision, and ease of use.
In contrast to slab gel electrophoresis, the Agilent 2100 bioanalyzer in conjunction with LabChip kits performs chipbased separations under standardized conditions, resulting in more reproducible results. Using prepackaged reagents and an assay specific software script that defines all instrumental parameters, a user can always obtain equivalent results for a given sample. This approach simplifies sample preparation, minimizes user errors, and facilitates the exchange of data between different users and laboratories.
In the context of LabChip Technology, several DNA sizing kits are available to cover a wide range of DNA fragment sizes. In addition to the DNA 500 and DNA 1000 kits, two other DNA LabChip kits are available: the DNA 7500 and DNA 12000 LabChip kits. They allow separation of DNA fragments up to 12000 base pairs. The DNA 500 LabChip kit for analysis of DNA fragments, ranging in size from 25 to 500 base pairs, and the DNA 1000 LabChip kit for analysis of DNA fragments, ranging in size from 25 to 1000 base pairs, supplement these two kits. The size range of the DNA 500 and DNA 1000 kits is especially useful for small to medium sized PCR and RT-PCR products and can resolve primer dimers. Other relevant applications
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