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High-content screening of GPCR activation with MetaXpress, AcuityXpress and the Transfluor assay system

L SUMMARY
The Z' factors (see Table 4) were calculated for both experiments.3 The Z' factor, a measure of assay quality, reflects both the data variation and the signal dynamic range. The Z' factors measured for this experiment are > 0.6, easily within the desirable range for a cell-based imaging assay.

Table 4. Z' Statistical Summary for Agonist Stimulation
and Antagonist Inhibition Agonist Dose-Response Z' Agonist Dose-Inhibition Z' Pit Phenotype Experiment 1 0.8 0.9 Experiment 2 0.9 0.8 Vesicle Phenotype Experiment 1 0.8 0.8 Experiment 2 0.7 0.9

DISCUSSION
The MetaXpress Transfluor module successfully identifies cells and measures pits and vesicles formed by beta-arrestin-GFP translocation to activated GPCRs in the Transfluor assay. Independent experiments with the beta-2-adrenergic receptor, both wild-type and enhanced, produce consistent EC50 and IC50 values with high Z' factors. These results demonstrate that the Transfluor assay as analyzed by MetaXpress is a highly robust and reproducible assay for GPCR activation.

The rapid acquisition, analysis and data visualization capabilities of MetaXpress and Acuity
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