METAXPRESS APPLICATION NOTE
By Susan Catalano, Ph.D., Acumen Pharmaceuticals, Sylvia de Bruin, Joe Bosworth, Ph.D.,
Neal Gliksman, Ph.D., Kimberly Best, Ph.D. and Paula Rickert, Ph.D., Molecular Devices Corporation,
1311 Orleans Drive, Sunnyvale, CA 94089.
The Transfluor Assay, validated on over 90 different GPCRs, uses a common pathway independent of downstream G-protein signaling which is shared by virtually all GPCRs, representing all classes of GPCRs and G proteins. The assay uses image analysis to quantitate internalization of GFP-tagged beta-arrestin that associates with the receptor of interest following activation.1 (See Figure 1.)
Unstimulated cells display diffuse cytoplasmic beta-arrestin-GFP fluorescence.
When an over-expressed GPCR is activated, beta-arrestin targets the receptor
for internalization, resulting in the appearance of small fluorescent
clathrin-coated pits. If the receptor has a high affinity for beta-arresti
Related biology technology :
1. siRNA screening of the cell cycle with two dynamic GFP sensors (from Discovery Matters Issue 1, July 2005)
2. Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3, 5 monophosphate and its validation for pharmacological screening at G protein-coupled receptors
3. Cell lines expressing recombinant aequorin and a G-protein coupled receptor for functional screening
4. Inactivation of Contaminating Templates in PCR Using High Intensity Ultraviolet Light
5. Enzyme Immunoassay for Studying Intracellular Levels of cAMP
6. LiquiChip Kits for bead-based cytokine and kinase assays
7. Quality control of antibodies using the 2100 bioanalyzer and the Protein 200 Plus assay
8. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
9. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
10. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
11. Is Z factor the best assessment for the quality of cellular assays delivering higher content?
(Date:7/30/2014)... (PRWEB) July 30, 2014 Selexis ... for biologic drug discovery and mammalian cell line development, ... CHO-Mplus Libraries ™ will be presented at the 10th ... being held, September 8 – 10, 2014 at the ... , The following abstract will be presented during ...
(Date:7/30/2014)... BCC Research ( http://www.bccresearch.com ) reveals in its ... Applications , the global market for industrial enzymes is ... registering a five-year compound annual growth rate (CAGR) of ... the detergent enzyme segment with a CAGR of 11.3% ... almost every sector of industrial activity, ranging from technical ...
(Date:7/30/2014)... 30, 2014 Researchers often use microelectronic ... but such devices can do much more – ... such, researchers have looked to patterned assemblies of ... metabolic engineering to characterize – and potentially control ... are envisioned to recreate animal and human physiological ...
(Date:7/30/2014)... 2014 Connecticut Center for Advanced Technology, ... East Hartford, Conn., was awarded a Rural Business Enterprise ... project that will determine the feasibility of implementing an ... USDA Rural Development provided $53,000 for CCAT under the ... phase of the feasibility study in fiscal year 2014. ...Breaking Biology Technology:Selexis SA to Present New Data and a Case Study at IBC’s Cell Line Development and Engineering Conference 2Selexis SA to Present New Data and a Case Study at IBC’s Cell Line Development and Engineering Conference 3Global Market for Industrial Enzymes to Reach Nearly $7.1 Billion by 2018; Detergent Enzyme Market to Record Maximum Growth 2Global Market for Industrial Enzymes to Reach Nearly $7.1 Billion by 2018; Detergent Enzyme Market to Record Maximum Growth 3UMD Researchers Bridge Gap between Microelectronics, Biological Systems 2UMD Researchers Bridge Gap between Microelectronics, Biological Systems 3UMD Researchers Bridge Gap between Microelectronics, Biological Systems 4USDA Grant Funds Second Phase of Bio-Energy Research Community Economic Development in CT 2USDA Grant Funds Second Phase of Bio-Energy Research Community Economic Development in CT 3
... SAN FRANCISCO, Nov. 12 VIA Pharmaceuticals,(Nasdaq: ... on the development of,compounds for the treatment ... its clinical advisory board. Joining the clinical ... Professor of Medicine,Harvard Medical School, and Senior ...
... Associated with Chronic Pain Conditions in Patients Who ... ... FRAZER, Pa., Nov. 12 Cephalon, Inc. (Nasdaq:,CEPH) today announced ... Food and Drug Administration (FDA) seeking approval to,market FENTORA(R) (fentanyl buccal ...
... N.J. -- The Edwin A. Stevens Society of Stevens Institute ... School of Systems and Enterprises, Dr. Dinesh Verma, with the ... at the Park Avenue Club in Florham Park, N.J., Nov. ... is by invitation only., The Presidents Leadership Award is presented ...Cached Biology Technology:VIA Pharmaceuticals Expands Clinical Advisory Board With Leading Experts in Cardiovascular Disease 2VIA Pharmaceuticals Expands Clinical Advisory Board With Leading Experts in Cardiovascular Disease 3VIA Pharmaceuticals Expands Clinical Advisory Board With Leading Experts in Cardiovascular Disease 4Cephalon Submits Supplemental New Drug Application for FENTORA 2Cephalon Submits Supplemental New Drug Application for FENTORA 3Cephalon Submits Supplemental New Drug Application for FENTORA 4Cephalon Submits Supplemental New Drug Application for FENTORA 5Cephalon Submits Supplemental New Drug Application for FENTORA 6Cephalon Submits Supplemental New Drug Application for FENTORA 7Dr. Dinesh Verma to be honored with President's Leadership Award at Edwin A. Stevens Society Gala 2Dr. Dinesh Verma to be honored with President's Leadership Award at Edwin A. Stevens Society Gala 3
(Date:7/30/2014)... iodine-125 (125I) in cancer treatment has been shown ... are critical for neural transmission between the peripheral ... colleagues from Institute of Radiation Medicine, Chinese Academy ... be implanted into rat dorsal root ganglia (DRG) ... with different radioactivity (0, 14.8, 29.6 MBq) were ...
(Date:7/30/2014)... first high-resolution map of the carbon stocks stored ... The new and improved methodology used to make ... market-based carbon economies. The new carbon map ... it provides the critical input to studies of ... and enforcement purposes. The technique includes the determination ...
(Date:7/29/2014)... researchers sometimes need to get back to basics. ... to study the impacts of the bumphead parrotfish ... at two remote locations in the central Pacific ... computer simulation, McCauley, an assistant professor in the ... his colleagues sought to understand whether the world,s ...Breaking Biology News(10 mins):Peru's carbon quantified: Economic and conservation boon 2Peru's carbon quantified: Economic and conservation boon 3Underwater elephants 2Underwater elephants 3
... University of Illinois scientists have learned that a specific ... health. "In experiments with mice, we knocked out ... body to make arachidonic acid. Without arachidonic acid, the ... itchy, they scratched themselves continuously, and they developed a ...
... A team of researchers from the United States and Europe ... a role in the etiology of common forms of kidney ... variations in DNA sequences in more than 65,000 individuals of ... be more frequent among people with poor kidney function or ...
... Just one "pulse" of artificial light at night disrupts ... by Dr. Rachel Ben-Shlomo of the University of Haifa-Oranim ... Charalambos P. Kyriacou of the University of Leicester. "Damage ... is therefore important to understand the causes of this ...Cached Biology News:U of I study: Lack of omega-6 fatty acid linked to severe dermatitis 2International research team discovers novel genes influencing kidney disease risk 2
... thermal cycler is a compact instrument for gene ... tubes, 12 x 0.5 ml tubes, or a ... a thumbwheel to adjust height and pressure to ... heat pumps provide excellent thermal performance, and the ...
MiniCycler thermal cycler with Hot Bonnet heated lid, holds 25x0.2ml tubes...
... with SR-101 Labeled Substrates (red fluorescence; ... measure serine protease activation • No ... FLISP Kits utilize cell-permeable, fluorescently labeled ... measure chymotrypsin-like activity in whole living ...
... MitoPT kit is used in conjunction with your ... and induce apoptosis according to your existing procedure ... a negative control). Once you have induced apoptosis ... to each population and incubate the cells for ...Biology Products: