METAXPRESS APPLICATION NOTE
By Susan Catalano, Ph.D., Acumen Pharmaceuticals, Sylvia de Bruin, Joe Bosworth, Ph.D.,
Neal Gliksman, Ph.D., Kimberly Best, Ph.D. and Paula Rickert, Ph.D., Molecular Devices Corporation,
1311 Orleans Drive, Sunnyvale, CA 94089.
The Transfluor Assay, validated on over 90 different GPCRs, uses a common pathway independent of downstream G-protein signaling which is shared by virtually all GPCRs, representing all classes of GPCRs and G proteins. The assay uses image analysis to quantitate internalization of GFP-tagged beta-arrestin that associates with the receptor of interest following activation.1 (See Figure 1.)
Unstimulated cells display diffuse cytoplasmic beta-arrestin-GFP fluorescence.
When an over-expressed GPCR is activated, beta-arrestin targets the receptor
for internalization, resulting in the appearance of small fluorescent
clathrin-coated pits. If the receptor has a high affinity for beta-arresti
Related biology technology :
1. siRNA screening of the cell cycle with two dynamic GFP sensors (from Discovery Matters Issue 1, July 2005)
2. Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3, 5 monophosphate and its validation for pharmacological screening at G protein-coupled receptors
3. Cell lines expressing recombinant aequorin and a G-protein coupled receptor for functional screening
4. Inactivation of Contaminating Templates in PCR Using High Intensity Ultraviolet Light
5. Enzyme Immunoassay for Studying Intracellular Levels of cAMP
6. LiquiChip Kits for bead-based cytokine and kinase assays
7. Quality control of antibodies using the 2100 bioanalyzer and the Protein 200 Plus assay
8. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
9. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
10. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
11. Is Z factor the best assessment for the quality of cellular assays delivering higher content?
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(Date:1/14/2014)... York, NY (PRWEB) January 14, 2014 ... initiated coverage of Alliqua, Inc. (OTCQB: ALQA). Alliqua ... and marketing proprietary products to serve the wound ... , In late 2012, Alliqua was restructured ... launched the company’s new strategy to become a ...Breaking Biology Technology:Xtalks Life Sciences Webinar Examines Safety Assessment of Biosimilars 2DTS Improves Efficiency for Life Science Document Translations 2Hepatitis B Foundation to Host Annual Crystal Ball Gala 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 3
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... Safety Study in Patients with Severe Post-Operative Pain; ... and Support NDA SubmissionSYDNEY and BEDMINSTER, N.J., Feb. ... QRXPY), a clinical-stage specialty pharmaceutical company focused on ... and central nervous system (CNS) disorders, announced today ...Cached Biology Technology:The Australian Research Council Selects Scopus for the Evaluation of the Australian Research Landscape 2The Australian Research Council Selects Scopus for the Evaluation of the Australian Research Landscape 3Gold-palladium nanoparticles achieve greener, smarter production of hydrogen peroxide 2Gold-palladium nanoparticles achieve greener, smarter production of hydrogen peroxide 3Gold-palladium nanoparticles achieve greener, smarter production of hydrogen peroxide 4QRxPharma Initiates Second Comparative Study for MoxDuo(TM) IR Dual-Opioid(TM) Pain Therapy 2QRxPharma Initiates Second Comparative Study for MoxDuo(TM) IR Dual-Opioid(TM) Pain Therapy 3QRxPharma Initiates Second Comparative Study for MoxDuo(TM) IR Dual-Opioid(TM) Pain Therapy 4
(Date:4/15/2014)... 2014, Irvine, Calif. UC Irvine neurobiologists have found ... when transplanted into the brains of mice with the ... is published in the journal Stem Cells Research ... to work in two different mouse models. , ... dementia, is associated with accumulation of the protein amyloid-beta ...
(Date:4/15/2014)... know as early as 2020 how high sea levels ... team of researchers including Florida International University scientist Rene ... Science Foundation,s (NSF) Florida Coastal Everglades Long-Term Ecological Research ... in ecosystems from coral reefs to deserts, mountains to ... sea level rise is one of the most certain ...
(Date:4/15/2014)... UC San Diego have succeeded in visualizing the ... for growth and resistance to drought. The achievement ... determine how the hormone helps plants respond to ... continuing increase in the atmosphere,s carbon dioxide, or ... in the April 15 issue of the scientific ...Breaking Biology News(10 mins):UCI study finds modified stem cells offer potential pathway to treat Alzheimer's disease 2Long-term predictions for Miami sea level rise could be available relatively soon 2Biologists develop nanosensors to visualize movements and distribution of plant hormone 2
... earths system of mid-ocean ridges plays a pivotal role in ... heat flow from the interior, and the chemistry of rock, ... that scientists discovered the existence of vast plumbing systems under ... spit it back out from seafloor ventsa process that brings ...
... of the most common human parasites, Toxoplasma gondii, uses a ... increase its numbers and when to remain dormant, researchers at ... The scientists report this week in Nature that they ... (ABA), with a plant herbicide. Low doses of the herbicide ...
... Association of Water Companies (NAWC) held its annual ... Bob Iacullo presented the 2007 Management Innovation Awards. ... to share innovative approaches to issues relevant to ... to United Water for the entry Senior Design ...Cached Biology News:Quakes under Pacific floor reveal unexpected circulatory system 2Quakes under Pacific floor reveal unexpected circulatory system 3Disrupting common parasites' ability to 'talk' to each other reduces infection 2Disrupting common parasites' ability to 'talk' to each other reduces infection 3United Water, in partnership with Stevens, wins Management Innovation Award 2
... (eIF4E-binding protein II) is involved in translation ... kidney. PHAS proteins bind to eIF-4E, the ... capped mRNA in vitro and in cells. ... of PHAS/eIF-4E complexes. This allows eIF-4E to ...
... 2 (VMAT2). An antibody made to the ... at ~55 kDa in postnuclear supernatants of ... wild type cells (2). Some additional bands, ... also detected and remain unaffected by the ...
... Anti-Matrix Metalloproteinase-2, Hinge Region By immunoblotting ... native protein. It reacts with free MMP-2 ... antibody buffered aqueous glycerol solution ... pH 7.4, containing 50% glycerol and 15 ...
Acetylcholinesterase from human and monkey....Biology Products: