METAXPRESS APPLICATION NOTE
By Susan Catalano, Ph.D., Acumen Pharmaceuticals, Sylvia de Bruin, Joe Bosworth, Ph.D.,
Neal Gliksman, Ph.D., Kimberly Best, Ph.D. and Paula Rickert, Ph.D., Molecular Devices Corporation,
1311 Orleans Drive, Sunnyvale, CA 94089.
The Transfluor Assay, validated on over 90 different GPCRs, uses a common pathway independent of downstream G-protein signaling which is shared by virtually all GPCRs, representing all classes of GPCRs and G proteins. The assay uses image analysis to quantitate internalization of GFP-tagged beta-arrestin that associates with the receptor of interest following activation.1 (See Figure 1.)
Unstimulated cells display diffuse cytoplasmic beta-arrestin-GFP fluorescence.
When an over-expressed GPCR is activated, beta-arrestin targets the receptor
for internalization, resulting in the appearance of small fluorescent
clathrin-coated pits. If the receptor has a high affinity for beta-arresti
Related biology technology :
1. siRNA screening of the cell cycle with two dynamic GFP sensors (from Discovery Matters Issue 1, July 2005)
2. Development of a sensitive enzyme fragment complementation assay for cyclic adenosine 3, 5 monophosphate and its validation for pharmacological screening at G protein-coupled receptors
3. Cell lines expressing recombinant aequorin and a G-protein coupled receptor for functional screening
4. Inactivation of Contaminating Templates in PCR Using High Intensity Ultraviolet Light
5. Enzyme Immunoassay for Studying Intracellular Levels of cAMP
6. LiquiChip Kits for bead-based cytokine and kinase assays
7. Quality control of antibodies using the 2100 bioanalyzer and the Protein 200 Plus assay
8. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
9. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
10. The development of a CXCR2 chemokine receptorbinding assay using the LEADseeker Multimodality Imaging System
11. Is Z factor the best assessment for the quality of cellular assays delivering higher content?
(Date:8/28/2014)... Whitehouse Laboratories is excited to announce ... PTI Inspection Systems that will enable it ... art leak testing method development and validation programs using ... As part of this agreement, Whitehouse Labs has taken ... Instrument developed and manufactured by PTI. The HVLD ...
(Date:8/27/2014)... YORK , Aug. 27, 2014 /PRNewswire/ ... research report is available in its catalogue: ... 2014-2018 http://www.reportlinker.com/p02316661/Global-Biodegradable-Packaging-Materials-Market-2014-2018.html ... materials are materials that are readily decomposed ... differs from conventional non-degradable packaging in terms ...
(Date:8/27/2014)... -- Research and Markets has announced the ... Polymer, Food & Beverage, Personal Care & Pharmaceutical) ... report to their offering. Lactic acid ... carbon atom. The main applications of lactic acid ... personal care product, and pharmaceutical industries. Lactic ...
(Date:8/27/2014)... LONDON , Aug. 27, 2014 ... The Graphene Opportunity - A Rational ... Is Graphene The New Nanotech? A similar ... predictions of applications ranging from microelectronics to water ... today announced the availability of the Graphene Opportunity ...Breaking Biology Technology:Whitehouse Labs Renews Partnership with PTI Inspection Systems 2Whitehouse Labs Renews Partnership with PTI Inspection Systems 3Global Biodegradable Packaging Materials Market 2014-2018 2Global Biodegradable Packaging Materials Market 2014-2018 3Global Biodegradable Packaging Materials Market 2014-2018 4Global Biodegradable Packaging Materials Market 2014-2018 5Global Biodegradable Packaging Materials Market 2014-2018 6Global Biodegradable Packaging Materials Market 2014-2018 7Global Lactic Acid (Biodegradable Polymer, Food & Beverage, Personal Care & Pharmaceutical) Market - Trends & Forecasts to 2019 2The Graphene Opportunity - A Rational View 2The Graphene Opportunity - A Rational View 3The Graphene Opportunity - A Rational View 4
... Advanced BioFuels Association applauded the leadership of U.S. Sen. Bill Nelson ... that extends the Investment Tax Credit to advanced biofuels. , ... The Nelson bill, S. 3338, would ... for companies whose projects, sole and exclusive purpose is to produce advanced ...
... Calif. , May 13 SRI International announced today ... grant from the Bill & Melinda Gates Foundation. The grant will ... Watson , Ph.D., protein biochemist in SRI,s Center for Advanced Drug ... , , ...
... ,, HARBIN, China , ... ("Renhuang" or the "Company"), a,developer, manufacturer and distributor of botanical ... successfully exhibited its products at the 63rd PHARMACHINA,fair("The Fair") held ... , , ...Cached Biology Technology:SRI International Receives $100,000 Grand Challenges Explorations Grant for Innovative Global Health Research 2SRI International Receives $100,000 Grand Challenges Explorations Grant for Innovative Global Health Research 3SRI International Receives $100,000 Grand Challenges Explorations Grant for Innovative Global Health Research 4SRI International Receives $100,000 Grand Challenges Explorations Grant for Innovative Global Health Research 5SRI International Receives $100,000 Grand Challenges Explorations Grant for Innovative Global Health Research 6Renhuang Pharmaceuticals Exhibits All-Natural Products at the 63rd PHARMACHINA Fair 2Renhuang Pharmaceuticals Exhibits All-Natural Products at the 63rd PHARMACHINA Fair 3Renhuang Pharmaceuticals Exhibits All-Natural Products at the 63rd PHARMACHINA Fair 4
(Date:8/28/2014)... more action is needed to ensure safeguards are in ... in PLOS ONE , researchers from Monash University, ... - combining results from different studies - to look ... areas, to determine whether they actually protect biodiversity. ... protecting an area from human exploitation made common sense, ...
(Date:8/27/2014)... Calif. , Aug. 27, 2014 WaferGen Bio-systems, ... its previously announced underwritten public offering of units of ... per unit for gross proceeds of $20 million, prior ... payable by the Company. The shares and warrants are ... Each unit sold in the offering consists of ...
(Date:8/27/2014)... Reportlinker.com announces that a new market ... Law Enforcement Biometrics Market in North America ... About Biometrics Biometrics is a technology that ... their physical or behavioral patterns. It involves the ... as fingerprints, face recognition, DNA, palm print, hand ...Breaking Biology News(10 mins):Protected areas proven to protect biodiversity 2WaferGen Bio-Systems Announces Successful Completion of Public Offering Raising $20 Million 2WaferGen Bio-Systems Announces Successful Completion of Public Offering Raising $20 Million 3Law Enforcement Biometrics Market in North America 2014-2018 2Law Enforcement Biometrics Market in North America 2014-2018 3Law Enforcement Biometrics Market in North America 2014-2018 4Law Enforcement Biometrics Market in North America 2014-2018 5
... increase the likelihood of immune complications following bone marrow ... November 22 advance online issue of Nature Genetics ... the donor,s genome but present in the recipient,s, transplants ... known as graft-versus-host disease, in which immune cells from ...
... and technological frontiers. The National Science Foundation has recently ... diverse efforts of the following investigators under the American ... researchers have been awarded grants for investigation of solar ... enhance the capabilities of NJIT,s Big Bear Solar Observatory ...
... to contribute their abstracts to the World Congress on ... meeting devoted to all aspects of osteoporosis and osteoarthritis ... in Florence, Italy from May 5-8, 2010. Beginning ... The submission deadline is February 4, ...Cached Biology News:Gene mismatch influences success of bone marrow transplants 2Gene mismatch influences success of bone marrow transplants 3Gene mismatch influences success of bone marrow transplants 4NJIT receives NSF funding to improve Big Bear Telescope, study solar energy 2Special sessions announced for World Congress on Osteoporosis 2010 - IOF WCO - ECCEO10 2Special sessions announced for World Congress on Osteoporosis 2010 - IOF WCO - ECCEO10 3
ANTI S. GROUP AG:H...
This kit only detects beta-galactosidase activity at pH 6 in cultured cells andtissue sections. Beta-galactosidase activity at pH 6 ispresent only in senescent cells, and is not found inpresenescent...
2,4,6, Trinitrophenyl hapten is conjugated to lipopolysaccharide (LPS)....
... This service is for when you need to ... increase their transformation efficiency. Our services for preparing ... your applications. MCLab's custom competent cell service offers: ... ) Fast turnaround Quality ...Biology Products: