METAXPRESS APPLICATION NOTE
By Susan Catalano, Ph.D., Acumen Pharmaceuticals, Sylvia de Bruin, Joe Bosworth, Ph.D.,
Neal Gliksman, Ph.D., Kimberly Best, Ph.D. and Paula Rickert, Ph.D., Molecular Devices Corporation,
1311 Orleans Drive, Sunnyvale, CA 94089.
The Transfluor Assay, validated on over 90 different GPCRs, uses a common pathway independent of downstream G-protein signaling which is shared by virtually all GPCRs, representing all classes of GPCRs and G proteins. The assay uses image analysis to quantitate internalization of GFP-tagged beta-arrestin that associates with the receptor of interest following activation.1 (See Figure 1.)
Unstimulated cells display diffuse cytoplasmic beta-arrestin-GFP fluorescence.
When an over-expressed GPCR is activated, beta-arrestin targets the receptor
for internalization, resulting in the appearance of small fluorescent
clathrin-coated pits. If the receptor has a high affinity for beta-arresti
Related biology technology :
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