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High Yield Transcription for Every Application

as assessed on the Agilent 2100 bioanalyzer. The single primary peak is indicative of product quality while the area under the peak is related to yield.

Figure 2. MEGAscript vs. Conventional In Vitro Transcription Reactions. 20 l transcription reactions containing 1 g template, T7 MEGAscript components and [32P]UTP, were incubated at 37C. 1 l aliquots were removed at 1 hr intervals and acid precipitable cpm determined by scintillation counting.


mMESSAGE mMACHINE: Translatable Capped RNA
For high yields of capped RNA, MEGAscript technology is incorporated into the mMESSAGE mMACHINE Kit. This kit contains an optimized ratio of cap analog to GTP, for the best trade off between yield and proportion of capped transcripts (80% capped). Furthermore, you can obtain 100% translatable capped RNA with the mMESSAGE mMACHINE T7 ULTRA Kit. This kit uses a unique cap analog that only incorporates in the functional, translatable orientation, resulting in the synthesis of capped RNAs that are 100% translatable.


MEGAscript RNAi: dsRNA Synthesis for RNAi
In one complete kit, you can generate ready-to-use double-stranded RNA (dsRNA) for RNAi experiments in non-mammalian systems. Unlike competing products, the MEGAscript RNAi Kit provides reagents to eliminate DNA template, single stranded RNA, and free nucleotides. The MEGAscript
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