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High Yield Transcription for Every Application

From the Recognized Leader in High Yield Transcription


The MEGAscript Kit is the most frequently cited large-scale transcription kit, and with good reason. It enables the consistent transcription of high yields of full-length RNA (Figure 1). Ambion offers a complete line of products specifically optimized to generate the following products:

capped RNA
dsRNA transcripts
uncapped RNA
amplified RNA
short RNA transcripts
milligram quantities of transcript


MEGAscript: Consistent, High Yields of RNA Transcripts
Using the MEGAscript Kit, you can produce over 120 g of RNA from 1 g of DNA template. This is 10-50 times the amount of RNA produced by conventional transcription (Figure 2). Reactions are versatile and can be manipulated to include specialized reagents such as modified nucleotides or additional polymerase. In addition, Ambion has optimized the transcription of short (20-500 nt) DNA templates with the MEGAshortscript Kit. This patented technology was designed to maximize the yield of short RNA transcripts from plasmid, oligonucleotides, and PCR product templates.

Figure 1. Electropherogram of RNA Transcripts of Different Sizes. In vitro transcription was performed using 1, 4, or 9 kb templates with Ambion's MEGAscript Kit according to protocol. 20 l reactions were diluted to 100 l and 1 l w
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