Navigation Links
High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation

th limiting nutrients can be pumped into the vessel at a rate that is capable of replenishing the nutrients that are depleted. Shakers can only run in a batch mode, meaning that the growth of the cells is limited by the nutrients present in the medium at the time of inoculation. The fermentors fed-batch mode further enables methanol flow rates to be controlled to condition the cells to the presence of the methanol, as well as provide methanol at the proper rate to allow addition of just enough methanol for protein synthesis while preventing excess methanol addition which can cause toxicity.

Researchers have found that optimum protein production in P. pastoris occurs at 30C, and that all protein expression ceases at 32C. However, high heat loads occur when P. pastoris is actively growing or expressing high levels of protein. In actively growing shake flask cultures, it is not uncommon for the temperature to increase 25C if left uncontrolled. Therefore, it is imperative that all aspects of the fermentor, including temperature controller, heat exchanger, vessel and piping, must be designed to regulate temperature for optimum protein production. In addition to the fermentors internal controller, an external bioprocessing software (BioCommand, New Brunswick Scientific) is routinely used to supervise the process, as well as to provide optimal nutrient feed rates, based on either the current status of the culture or to actuate pre-determined control scenarios.


Fermentation Protocol
Research was conducted in BioFlo 3000 benchtop fermentors (New Brunswick Scientific) (Figure 1) with interchangeable, autoclavable vessels of 1.25 to 10 L working volume, as well as in a BioFlo 4500 fermentors with sterilizable-in-place vessels of 15 L and 20 L working volume, (New Brunswick Scientific
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Yeast Protein Production System Features High Yields and One-Step Purification
2. Efficient Cleavage of Fusion Proteins to Yield Native Amino Termini
3. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
4. ArrayScript RT: Maximize Yields of Full-length cDNA
5. Increase Protein Activity and Yield
6. High Yield Transcription for Every Application
7. Maximize Viral RNA Yield from Biological Fluid
8. Recover High Yields of Total Nucleic Acid from Formalin-fixed, Paraffin-embedded (FFPE) Tissue
9. Synthesize High Yields of Biotinylated aRNA
10. Translate High Yields of Active Protein
11. Translate High Yields of Biologically Active Proteins
Post Your Comments:
(Date:7/10/2014)... 2014  Kainos Capital, a firm specializing in acquiring ... it has acquired the Slim-Fast brand from Unilever. Unilever ... of the transaction were not disclosed. Slim-Fast ... that markets ready-to-drink shakes, powders, bars and snacks to ... and in the United Kingdom ...
(Date:7/10/2014)... 2014 -- According to the International Atomic Energy ... comes from terrorists acquiring sufficient quantities of plutonium ... crude nuclear explosive device. The IAEA also notes ... involved gram-level quantities, which can be challenging to ... a new study appearing this week in the ...
(Date:7/10/2014)... the basis of psychiatric disorders has been extremely challenging ... risk but are insufficient to cause disease. Now investigators ... Press journal Cell Stem Cell describe a ... risks interact with other risk factors or environmental exposures ... research pinpoints a genetic variant that may predispose individuals ...
(Date:7/10/2014)... out for crude 3-D glasses, polarized glasses, and ... basic devices, used to trick the brain into ... rendered obsolete with the introduction of new holography ... TAU doctoral students Yuval Yifat, Michal Eitan, and ... on nanoantennas that could be used for security ...
Breaking Biology Technology:Kainos Capital Acquires Slim-Fast From Unilever 2Sensitive detection method may help impede illicit nuclear trafficking 2New strategy could uncover genes at the root of psychiatric illnesses 2Projecting a 3-dimensional future 2Projecting a 3-dimensional future 3
... has succeeded in significantly increasing the sensitivity of the ... on this topic at Delft University of Technology on ... and astronomers from around the world are currently working ... of 66 advanced telescopes which will be placed at ...
... Iceland, February 3 deCODE genetics,(Nasdaq: DCGN ) ... letter from,the Nasdaq Listing Qualifications Panel (the "Panel") stating ... stock from the Nasdaq Global,Market to the Nasdaq Capital ... Wednesday, February 4, 2009. The Nasdaq Capital Market, which ...
... DHA (docosahexaenoic acid) omega-3 innovator and maker of the ... today that it has entered into a license agreement ... is a process widely used in food manufacturing that ... the nutritional value, shelf-life and flavor of the finished ...
Cached Biology Technology:Nanotechnology makes supertelescopes much more sensitive 2deCODE Announces Transfer of the Listing of its Common Stock from Nasdaq Global Market to Nasdaq Capital Market 2deCODE Announces Transfer of the Listing of its Common Stock from Nasdaq Global Market to Nasdaq Capital Market 3Martek, General Mills Collaborate on Microencapsulation Technology for Food Applications 2Martek, General Mills Collaborate on Microencapsulation Technology for Food Applications 3
(Date:7/11/2014)... There is evidence that under the normal ... and development, maintain neuronal environment, and exhibit ... and neurodegenerative disease. Previous studies have found ... cells after chemical induction have reduced viability, ... application. Prof. Xiaodong Yuan, Kailuan General Hospital, ...
(Date:7/11/2014)... that let nerve cells send out electrical signals, Johns ... tactic that may offer a new way to protect ... responsible way. , Their findingthat naturally occurring insect ... for a closely related onesuggests that insecticides can be ... like bees. A summary of the research, led by ...
(Date:7/10/2014)... 27, 2014  The American Academy of Forensic ... Standards and Technology (NIST) and the Department of ... and forensic science expert members to the Forensic ... element of the NIST,s Organization of Scientific Area ... first appointments made to the new FSSB that ...
Breaking Biology News(10 mins):Potent spider toxin 'electrocutes' German, not American, cockroaches 2Potent spider toxin 'electrocutes' German, not American, cockroaches 3Ten Members of the American Academy of Forensic Sciences Appointed to Forensic Science Standards Board (FSSB) 2Ten Members of the American Academy of Forensic Sciences Appointed to Forensic Science Standards Board (FSSB) 3
... we,ve been told, eliminating or severely limiting fats from ... in patients suffering from heart failure, a study at ... Results from biological model studies conducted by assistant professor ... researchers, demonstrate that a high-fat diet improved overall mechanical ...
... WASHINGTON The Chesapeake Bay Program is a ... jurisdictions in the bay watershed to oversee the restoration ... the extent of pollutants -- such as nitrogen, phosphorus, ... from the National Research Council assesses the framework used ...
... a researcher at the Royal Swedish Academy of Sciences, Margareta ... permafrost. "The changes we see are dramatic. And ... from the norm when compared with a longer term perspective", ... of the globe that is warming up fastest today. Measurements ...
Cached Biology News:Damaged hearts pump better when fueled with fats 2Chesapeake Bay program's 2-year milestones improve upon past strategies, but accounting of progress remains a challenge 2A new research report shows effects of climate change in the Arctic are more extensive than expected 2A new research report shows effects of climate change in the Arctic are more extensive than expected 3
... CLS number is a new ... match Cornings product number. If ... order under the old Sigma-Aldrich ... service for assistance. ID clarifier: ...
ID clarifier: With ethidium bromide (50 μg/ml)...
... Pre-tested for optimal ... support the growth of ... for methylcellulose colony-forming assays ... hematopoietic cells. Not suitable ...
... dual parameter assay for mitochondrial membrane ... Active caspases are detected by carboxyfluorescein ... are cell-permeable, non-cytotoxic and bind covalently ... cells. At the same time the ...
Biology Products: