Navigation Links
High-Titer ,Retroviral Vectors for Gene Delivery

ted samples were selected with 1 mg/ml of G418 for 10 days, then the colonies were stained with methylene blue and counted. The titer, as determined by G418 resistance, was identical to that determined by FACS (1 x 107 cfu/ml) (data not shown).

Fig.4

To assess expression levels from the pFB vectors, the GFP coding sequence was inserted into the MMLV vector pLXSN,10 and viral titers were determined for the derivative pLXSN-GFP and pFB-Neo-GFP. NIH3T3 cells were subsequently infected with an equivalent number of virus particles for both vectors, and expression levels were qualitatively measured by fluorescence intensity in the population as a whole by FACS (Figure 4, Panel A and Panel B) and in individual cells by fluorescence microscopy (Figure 4, Panel C and Panel D). As the results in Figure 4 indicate, the peak fluorescence intensity is several-fold higher for cells infected with pFB-Neo-GFP and is also significantly higher on a cell-to-cell basis for this vector.

Conclusions

Using the vectors pFB and pFB-Neo for high-titer virus production will, in many cases, obviate the need for scale-up production and tedious concentration steps when large numbers of virus particles are required (e.g., production of cDNA expression libraries). In addition, the relatively high expression levels achieved with these vectors will facilitate library screening and enhance general-purpose gene expression in infected cells. Because the vectors may be used with any MMLV-based packaging line, virus can be produced that infects virtually any cell type. The relatively small amount of sequence between the LTRs allows for expression of sequence of up to 8 kb without a significant loss of titer. The small size also facilitat
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Safe Production of High-Titer Retrovirus
2. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
3. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
4. Epitope-Tagging Vectors for Functional Analysis in Yeast
5. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
6. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
7. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
8. Versatile Reporter Vectors for Monitoring Viral Transduction
9. Improved Vectors for PathDetect Trans-Reporting Systems
10. Mammalian Expression Vectors with Interchangeable Selectable Markers
11. Delivering siRNA Using Adenoviral Vectors
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: High Titer Retroviral Vectors for Gene Delivery

(Date:7/13/2014)... 2014 Deep Research Report on Global and ... study on the current state of the Nano Silver ... a basic overview of the industry, including definitions, applications ... domestic market analysis are provided with a focus on ... A comparison between the international and Chinese situation is ...
(Date:7/11/2014)... WEST PALM BEACH, Fla. , July 11, 2014 ... wide variety of high-quality peptides that are used solely ... online. To honor two successful years in business, Maxim ... Performance LLC, has just launched a new and updated ... addition, the research blog section has been updated with ...
(Date:7/11/2014)... DUBLIN , July 11, 2014 ... "International Transfection Technology Market - Global Industry Analysis, ... report to their offering. ... to introduce nucleic acids (either DNA or RNA) into ... therapy, mutation of cancer cells and protein metabolism by ...
(Date:7/11/2014)... 2014 EPFL and the University of  ... of John P. Donoghue . The American neuroscientist ... . The new Wyss Center for ... Geneva , has named as its director ... P. Donoghue , founder of Brown University,s Institute for ...
Breaking Biology Technology:2014 Nano Silver Industry in Global and China Regions Development Proposals Analysis Available at DeepResearchReports.com 22014 Nano Silver Industry in Global and China Regions Development Proposals Analysis Available at DeepResearchReports.com 3Maxim Peptide Celebrates Second Anniversary Online with an Updated Website and Special Deals Section 2International Transfection Technology Market - Industry Analysis, Size, Share, Growth, Trends and Forecast to 2019 2John P. Donoghue is Taking the Lead at the Wyss Center and is Appointed Professor at EPFL and University of Geneva 2John P. Donoghue is Taking the Lead at the Wyss Center and is Appointed Professor at EPFL and University of Geneva 3
... Institute of Standards and Technology (NIST) may have ... of computer memory now under development. The work, ... University (GMU), aims to optimize nanowire-based charge-trapping memory ... computers and cell phones that can operate for ...
... DIEGO, May 25, 2011 Anadys Pharmaceuticals, Inc. (Nasdaq: ... for the treatment of hepatitis C, announced today that it ... ANA773, the Company,s oral immuno-modulator that acts via the TLR-7 ... Annual Meeting in Chicago, IL.  The data from the completed ...
... 2011 WaferGen Biosystems, Inc. (OTCBB:WGBS), a leading ... it has entered into definitive agreements with three ... LLC, Deerfield Management and Merlin Nexus, and certain ... $30.4 million private placement financing of equity and ...
Cached Biology Technology:NIST 'nanowire' measurements could improve computer memory 2Anadys Highlights ANA773 Data to be Presented at ASCO 2Anadys Highlights ANA773 Data to be Presented at ASCO 3Anadys Highlights ANA773 Data to be Presented at ASCO 4WaferGen Announces $30 Million Financing 2WaferGen Announces $30 Million Financing 3WaferGen Announces $30 Million Financing 4
(Date:7/10/2014)... , June 30, 2014  Aware, Inc. (NASDAQ: ... software and services, previously announced on June 26, 2014 ... cash dividend of $1.75 per share, or approximately $40 ... of July 10, 2014 and a payment date of ... NASDAQ that it had set an ex-dividend date for ...
(Date:7/10/2014)... SHELTON, Conn. , July 2, 2014 NXT-ID, Inc., ... market, reports that CEO Mr. Gino Pereira was ... radio show in Tampa Florida . Mr. ... radio host Cedric Harris and how the Wocket™ ... secure alternative.  Cedric Harris tells ...
(Date:7/10/2014)... 2014 According to a new ... Control Systems Market Global Forecast, Market Share, Size, Growth ... Access Control systems market was valued at USD 15,406.1 ... a CAGR of 12.6% from 2014 to 2019, to ... 2019. Browse the full Electronic Access Control ...
Breaking Biology News(10 mins):NASDAQ Sets Ex-Dividend Date for Aware, Inc.'s Special Cash Dividend of $1.75 Per Share 2Security and Your Money: Tampa Bay Radio Host Cedric Harris Talks to NXT-ID CEO Mr. Gino Pereira about the Wocket Smart Wallet 2Security and Your Money: Tampa Bay Radio Host Cedric Harris Talks to NXT-ID CEO Mr. Gino Pereira about the Wocket Smart Wallet 3Security and Your Money: Tampa Bay Radio Host Cedric Harris Talks to NXT-ID CEO Mr. Gino Pereira about the Wocket Smart Wallet 4Electronic Access Control Systems Market is Expected to Reach USD 31.2 Billion Globally in 2019: Transparency Market Research 2Electronic Access Control Systems Market is Expected to Reach USD 31.2 Billion Globally in 2019: Transparency Market Research 3Electronic Access Control Systems Market is Expected to Reach USD 31.2 Billion Globally in 2019: Transparency Market Research 4Electronic Access Control Systems Market is Expected to Reach USD 31.2 Billion Globally in 2019: Transparency Market Research 5
... The California Institute for Regenerative Medicine (CIRM) has awarded ... associate professor at Sanford-Burnham Medical Research Institute (Sanford-Burnham). Chen,s ... part of CIRM,s Basic Biology IV awards program, which ... stem cells and how to work with them. This ...
... study tissue engineering and test new drugs often need ... They can do this by prodding the cells into ... the desired direction with acoustic waves, electric fields, or ... can position individual cells with a high level of ...
... , WEST LAFAYETTE, Ind. Researchers have created a new ... be used in drug-delivery patches powered by fermentation., The micropump ... water is added and the patch is placed on the ... yeast and sugar to ferment, generating a small amount of ...
Cached Biology News:California's stem cell agency boosts heart disease research at Sanford-Burnham 2Body heat, fermentation drive new drug-delivery 'micropump' 2
Mouse Collagen IV...
The Captivate Disposable Sample Chamber is designed for use with the Captivate magnetic yoke assembly (C-24700) and can be used with our Captivate ferrofluid conjugates (C-21473 C-21474 C-21476) or ...
Sheep Serum US Origin...
Human PDGF, CF...
Biology Products: