Navigation Links
High-Titer ,Retroviral Vectors for Gene Delivery

s of up to 8 kb without a significant loss of titer.1 Because the titers of these pMFG-based minimal vectors are sufficiently high, allowing greater than or equal to 90% of target cells to be infected, the need for antibiotic selection is essentially obviated.

Titer Determination

Fig.3

Coding sequence for the enhanced green fluorescent protein (GFP)9 was inserted between the EcoR I and BamH I sites in pFB and pFB-Neo. The 293 cell-based amphotropic retroviral producer cell line HW293-A (unpublished data) was used for virus production. Producer cells were transiently transfected with both of the GFP constructs using the Transfection MBS mammalian transfection kit (Stratagene) with some modifications.4 Two days following transfection, viral supernatants were collected, and serial dilutions of each supernatant were used to infect dividing NIH3T3 cells. Two days later, the infected NIH3T3 cell populations were analyzed by fluorescence-activated cell sorting (FACS) (Figure 3). Titers were determined using the highest dilution for which cells containing fluorescence above background are clearly visible in the upper left (UL) quadrant of the plot (1:105 for both viruses). Titers are expressed as the product of the dilution factor multiplied by the percentage of infected cells that fluoresce above background, then multiplied by the number of cells infected. By this definition, pFB-Neo-GFP infected cells showed a titer of 1 x 107 cfu/ml of supernatant, and pFB-GFP gave a titer of 3 x 108 cfu/ml. This experiment was repeated with essentially identical results (data not shown). In addition, for the vector pFB-Neo-GFP, parallel infec
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Safe Production of High-Titer Retrovirus
2. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
3. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
4. Epitope-Tagging Vectors for Functional Analysis in Yeast
5. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
6. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
7. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
8. Versatile Reporter Vectors for Monitoring Viral Transduction
9. Improved Vectors for PathDetect Trans-Reporting Systems
10. Mammalian Expression Vectors with Interchangeable Selectable Markers
11. Delivering siRNA Using Adenoviral Vectors
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: High Titer Retroviral Vectors for Gene Delivery

(Date:10/30/2014)... Illinois (PRWEB) October 30, 2014 ... and Cellular Medicine Showcase in Chicago, leading applied ... Orthopedic Surgeon, Dr. Wade McKenna presented talks on ... and Orthopedic Surgical Applications For Stem Cells. , ... amniotic membrane and the properties of AlphaGEMS that ...
(Date:10/30/2014)... October 30, 2014 Spartan Bioscience announced ... RX CYP2C19 System . It detects CYP2C19 genetic mutations ... swab. , The Spartan RX CYP2C19 System is the ... been approved in Canada. Due to the system’s ease ... be used by healthcare professionals such as doctors, nurses, ...
(Date:10/30/2014)... -- Isis Pharmaceuticals, Inc. (NASDAQ: ISIS ), the ... a company overview at the Nomura Biotechnology Conference on Thursday, ... Boston, MA. A live webcast of ... section of the Company,s website, www.isispharm.com .  A replay ... and will be archived for a limited time. ...
(Date:10/30/2014)... that devastated a wide swath of Colorado last year ... and other infrastructure had been upgraded or modernized, according ... Denver. , "People need to understand the importance and ... of structural engineering at the CU Denver College of ... "There is an assumption that a bridge will stand ...
Breaking Biology Technology:Riordan-McKenna Institute Founders, Neil Riordan, PhD and Orthopedic Surgeon, Dr. Wade McKenna Present at the Mid American Regenerative and Cellular Medicine Showcase 2Riordan-McKenna Institute Founders, Neil Riordan, PhD and Orthopedic Surgeon, Dr. Wade McKenna Present at the Mid American Regenerative and Cellular Medicine Showcase 3Riordan-McKenna Institute Founders, Neil Riordan, PhD and Orthopedic Surgeon, Dr. Wade McKenna Present at the Mid American Regenerative and Cellular Medicine Showcase 4Health Canada approves first near-patient DNA test for personalized medicine 2Health Canada approves first near-patient DNA test for personalized medicine 3Isis Pharmaceuticals to Present at the Nomura Biotechnology Conference 2CU Denver study says upgrading infrastructure could reduce flood damage 2
... , PETERSBURG, Va., Nov. 4 Star Scientific, ... the company plans to introduce the CigRx(TM) nutraceutical product developed ... sales in partnership with inVentiv Health, Inc ... of commercialization solutions for every stage of the product lifecycle, ...
... EMERYVILLE, Calif., Nov. 4 Onyx Pharmaceuticals, Inc. (Nasdaq: ... at the Credit Suisse 18th Annual Healthcare Conference on ... a.m. Pacific Time). Interested parties may access a ... http://www.onyx-pharm.com/view.cfm/32/Event-Calendar , It is recommended ...
... , NORRISTOWN, Pa., Nov. 4 ... company, today announced that it has formed a strategic ... contract research organization that specializes in the agrichemical and ... The exclusive partnership leverages the expertise of industry veteran ...
Cached Biology Technology:Star Scientific Plans Worldwide Marketing and Sales of CigRx(TM) Nutraceutical in Partnership with inVentiv Health 2Star Scientific Plans Worldwide Marketing and Sales of CigRx(TM) Nutraceutical in Partnership with inVentiv Health 3Star Scientific Plans Worldwide Marketing and Sales of CigRx(TM) Nutraceutical in Partnership with inVentiv Health 4Emerson Resources Forges Strategic Partnership with Bio Research 2
(Date:10/29/2014)... by unconventional oil and gas production are well over ... the open access journal Environmental Health . High ... The study is the first to be based on ... and could be used to supplement official air-quality monitoring ... that do not readily flow to the surface. This ...
(Date:10/29/2014)... 29, 2014, Beaverton, OR) New research presented ... International Conference in Houston, TX showed for the ... HPV infection in women using a readily available ... study, presented by Dr. Judith A. Smith, Pharm.D., ... and Reproductive Sciences at The University of Texas ...
(Date:10/29/2014)... to make their food, but they also need protection from ... a group of molecules in plants that shields them from ... of the American Chemical Society , one team reports on ... Timothy Zwier and colleagues at Purdue University note that the ... serious damage to plant DNA and, as a result, hinder ...
Breaking Biology News(10 mins):Air quality and unconventional oil and gas sites 2Air quality and unconventional oil and gas sites 3HPV infections in women eradicated by AHCC, Japanese mushroom extract 2
... COLD SPRING HARBOR, N.Y. (Jan. 5, 2011) Auxin is ... all aspects of plant development and physiology. A new book, ... broad overview of auxin and its many roles in plant biology. ... of plant research," write the editors, Mark Estelle, Dolf Weijers, Karin ...
... in children requires special knowledge of growth physiology. Incorrect ... is less often caused by technical deficiencies than by ... group. Using the example of treating fractures of the ... Center, and Lucas Wessel, University Medical Center Mannheim, report ...
... research, in association with Elsevier, the leading publisher of ... the 7th NIZO Dairy conference, to be held on ... The conference aims to combine the most recent scientific ... a unique opportunity for experts from academia and the ...
Cached Biology News:7th NIZO Dairy Conference to focus on flavor and texture, innovations in dairy 2
Proliferin (C-14)...
Porcine Pulmonary Artery Endothelial Cells (PPAEC) (>500,000 cells)...
... is a dedicated fluorometer with ... kinds of assays in industrial ... clinical applications. Cell biology, immunology ... important application areas. Robot-friendly hardware ...
... Thermo Savant's latest SpeedVac Concentrator, the ... and adds a new modern twist., The ... controller and gauge for optimizing runs; preventing ... to be stopped at the end-users preset ...
Biology Products: